Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001000.1:
Cluster 1NRP / Polyketide141964

BGC0001000, hectochlorin biosynthetic gene cluster from Lyngbya majuscula. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: hectochlorin
PubChem ID: 636718
SMILES string: Copy to clipboard
Molecular formula: C27H34Cl2N2O9S2
Average molecular mass: 665.594 Da
Molecular activity: Antifungal, Cytotoxic
Molecular target: actin

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Other lipopeptide (cyclic)
Thioesterase type:
Type I
Release / cyclization type:
Hydrolysis

Nonribosomal peptide synthetases:
HctA (AAY42393.1)
Module 0
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: Starter
HctE (AAY42397.1)
Module 2
A specificity: 2-oxo-isovaleric acid
Evidence for specificity: Activity assay
C domain subtype: LCL
Module 3
A specificity: Cysteine
Evidence for specificity: Activity assay
C domain subtype: Heterocyclization
Scaffold-modifying domain: Oxidation
HctF (AAY42398.1)
Module 4
A specificity: 2-oxo-isovaleric acid
Evidence for specificity: Activity assay
C domain subtype: LCL
Module 5
A specificity: Cysteine
Evidence for specificity: Activity assay
C domain subtype: Heterocyclization
Scaffold-modifying domain: Oxidation
Polyketide subclass:
Other (cyclic)
Polyketide synthase subclass:
Modular type I
Starter unit:
Other
Thioesterase type:
None
Release / cyclization type:
Hydrolysis

Modular polyketide synthases:
HctD (AAY42396.1)
Module 1
AT specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
KR stereochemistry: A-group
Scaffold-modifying domain: Methylation

Gene cluster description

hectochlorin (BGC0001000). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank AY974560. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Sequence-based prediction
MIxS-compliance:Unknown
Comments:The phylogeny/classification of the producing organism for this molecule has been updated from Lyngbya majuscula JHB to Moorea producens JHB.
Contact for this cluster:Emily A. Monroe (William Paterson University)

Literature references

1. Ramaswamy AV et al. (2007) Cloning and biochemical characterization of the hectochlorin biosynthetic gene cluster from the marine cyanobacterium Lyngbya majuscula. J Nat Prod 70(12):1977-86. doi: 10.1021/np0704250. Epub 2007 Nov 15.
2. Marquez BL et al. (2002) Structure and absolute stereochemistry of hectochlorin, a potent stimulator of actin assembly. J Nat Prod 65(6):866-71.