BGC0001169 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001169 (Streptomyces marincola)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	49,160	High	piericidin A1	PKS

BGC0001169 - Region 1 - T1PKS

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Location: 1 - 49,160 nt. (total: 49,160 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 49,161
Label		BGC0001169

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

AHE80993.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

AHE80994.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

AHE81001.1: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acylase methyltransferase

AHE80996.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

AHE81000.1: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: hydrolase

AHE80999.1: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Ligases: synthase

AHE80991.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

AHE80992.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AHE80995.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001169.5	1.00	PKS	piericidin A1	Streptomyces sp. SCSIO 03032
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000124.5	0.72	PKS	piericidin A1	Streptomyces piomogenus
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001169.5	1.00	PKS	piericidin A1	Streptomyces sp. SCSIO 03032
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000124.5	0.72	PKS	piericidin A1	Streptomyces piomogenus
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 49160:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ccmal) + (Me-ohmal - Me-ccmal) + (pk) + (pk) + (ccmal) + (Me-mal - mal)
Non-colinear ordering:: AHE80991.1 + AHE80992.1 + AHE80993.1 + AHE80994.1 + AHE80995.1 + AHE80996.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AHE80991.1: mmal - mmal

PKS_AT (208..507): mmal

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 75.0%; 2-Methylbutyryl-CoA: 66.7%; Methoxymalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 149.7
Ethylmalonyl-CoA: 144.7
Propionyl-CoA: 122.4
Methoxymalonyl-CoA: 120.2
2-Methylbutyryl-CoA: 119.4
CHC-CoA: 103.8
trans-1,2-CPDA: 94.6
Malonyl-CoA: 71.4
Isobutyryl-CoA: 71.3
Benzoyl-CoA: 41.3
Acetyl-CoA: 35.1
fatty_acid: 28.8
inactive: 16.3
3-Methylbutyryl-CoA: 8.8

PKS_AT (1194..1470): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 174.7
Ethylmalonyl-CoA: 161.2
Methoxymalonyl-CoA: 133.8
2-Methylbutyryl-CoA: 107.5
trans-1,2-CPDA: 92.3
Propionyl-CoA: 86.8
CHC-CoA: 72.5
Malonyl-CoA: 64.4
Isobutyryl-CoA: 56.7
Benzoyl-CoA: 37.4
inactive: 32.7
Acetyl-CoA: 27.6
fatty_acid: 27.0
3-Methylbutyryl-CoA: 11.6

AHE80992.1: mmal - mmal

PKS_AT (584..860): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 174.7
Ethylmalonyl-CoA: 161.2
Methoxymalonyl-CoA: 133.8
2-Methylbutyryl-CoA: 107.5
trans-1,2-CPDA: 92.3
Propionyl-CoA: 86.8
CHC-CoA: 72.5
Malonyl-CoA: 64.4
Isobutyryl-CoA: 56.7
Benzoyl-CoA: 37.4
inactive: 32.7
Acetyl-CoA: 27.6
fatty_acid: 27.0
3-Methylbutyryl-CoA: 11.6

PKS_AT (2119..2395): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 174.7
Ethylmalonyl-CoA: 161.2
Methoxymalonyl-CoA: 133.8
2-Methylbutyryl-CoA: 107.5
trans-1,2-CPDA: 92.3
Propionyl-CoA: 86.8
CHC-CoA: 72.5
Malonyl-CoA: 64.4
Isobutyryl-CoA: 56.7
Benzoyl-CoA: 37.4
inactive: 32.7
Acetyl-CoA: 27.6
fatty_acid: 27.0
3-Methylbutyryl-CoA: 11.6

AHE80993.1: pk

PKS_AT (572..724): pk

ATSignature: (unknown)

No matches above 50%

minowa: CHC-CoA

Prediction, score:

CHC-CoA: 22.2
Methylmalonyl-CoA: 17.0
trans-1,2-CPDA: 16.2
Propionyl-CoA: 13.1
Methoxymalonyl-CoA: 12.6
2-Methylbutyryl-CoA: 12.4
inactive: 0.0
fatty_acid: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.9

AHE80994.1: pk

PKS_AT (554..824): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 213.6
Methylmalonyl-CoA: 212.7
Methoxymalonyl-CoA: 165.7
2-Methylbutyryl-CoA: 161.0
trans-1,2-CPDA: 135.6
Propionyl-CoA: 113.9
CHC-CoA: 99.9
Malonyl-CoA: 85.6
Isobutyryl-CoA: 85.5
Benzoyl-CoA: 70.4
Acetyl-CoA: 49.4
3-Methylbutyryl-CoA: 34.6
fatty_acid: 28.7
inactive: 26.3

AHE80995.1: mal

PKS_AT (570..883): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 190.7
inactive: 87.8
Methoxymalonyl-CoA: 47.9
Methylmalonyl-CoA: 38.6
Propionyl-CoA: 25.4
Benzoyl-CoA: 11.3
fatty_acid: 11.0
Acetyl-CoA: 10.4
Isobutyryl-CoA: 9.6
3-Methylbutyryl-CoA: 6.7
CHC-CoA: 4.1
Ethylmalonyl-CoA: 4.0
2-Methylbutyryl-CoA: 2.6
trans-1,2-CPDA: 0.0

AHE80996.1: mmal - mal

PKS_AT (595..871): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 174.7
Ethylmalonyl-CoA: 161.2
Methoxymalonyl-CoA: 133.8
2-Methylbutyryl-CoA: 107.5
trans-1,2-CPDA: 92.3
Propionyl-CoA: 86.8
CHC-CoA: 72.5
Malonyl-CoA: 64.4
Isobutyryl-CoA: 56.7
Benzoyl-CoA: 37.4
inactive: 32.7
Acetyl-CoA: 27.6
fatty_acid: 27.0
3-Methylbutyryl-CoA: 11.6

PKS_AT (1608..1910): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 165.7
inactive: 75.3
Methoxymalonyl-CoA: 47.7
Methylmalonyl-CoA: 37.4
Propionyl-CoA: 22.0
fatty_acid: 11.2
Isobutyryl-CoA: 10.2
Acetyl-CoA: 10.2
Ethylmalonyl-CoA: 10.0
Benzoyl-CoA: 8.1
2-Methylbutyryl-CoA: 6.5
CHC-CoA: 5.1
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor