BGC0001194 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001194 (Embleya scabrispora)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	72,530	cremimycin	Polyketide	47%

BGC0001194 - Region 1 - T1PKS

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Location: 1 - 72,530 nt. (total: 72,530 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 72,531
Label		BGC0001194

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001194.1	0.81	Polyketide	hitachimycin	Streptomyces scabrisporus
BGC0000041.1	0.32	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001164.1	0.31	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001273.1	0.30	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276.1	0.30	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.1	0.30	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001275.1	0.29	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000120.1	0.29	Polyketide	patulin	Penicillium expansum
BGC0000018.1	0.29	Polyketide	angolamycin	Streptomyces eurythermus
BGC0000160.1	0.29	Polyketide	terreic acid	Aspergillus terreus NIH2624

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001194.1	0.93	Polyketide	hitachimycin	Streptomyces scabrisporus
BGC0000041.1	0.65	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001164.1	0.65	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001273.1	0.63	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276.1	0.63	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.1	0.63	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001275.1	0.63	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000120.1	0.63	Polyketide	patulin	Penicillium expansum
BGC0000018.1	0.63	Polyketide	angolamycin	Streptomyces eurythermus
BGC0000160.1	0.62	Polyketide	terreic acid	Aspergillus terreus NIH2624

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 72530:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ccmal - redmal) + (ohmal - ccmal) + (ohmal) + (ccmal - pk - ccmal) + (ccmal)
Non-colinear ordering:: hitP1 + hitP2 + hitP5 + hitP4 + hitP3

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

hitP1

PKS_AT (670..960): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 205.3
inactive: 120.5
Methoxymalonyl-CoA: 65.2
Methylmalonyl-CoA: 60.1
Propionyl-CoA: 46.1
2-Methylbutyryl-CoA: 22.9
trans-1,2-CPDA: 16.9
CHC-CoA: 15.6
Ethylmalonyl-CoA: 15.2
Isobutyryl-CoA: 12.7
Acetyl-CoA: 11.4
Benzoyl-CoA: 10.1
fatty_acid: 7.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (2392..2683): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 197.9
inactive: 122.4
Methoxymalonyl-CoA: 57.5
Methylmalonyl-CoA: 47.0
Propionyl-CoA: 31.9
Ethylmalonyl-CoA: 27.0
Acetyl-CoA: 21.1
Benzoyl-CoA: 19.9
Isobutyryl-CoA: 19.8
2-Methylbutyryl-CoA: 17.7
CHC-CoA: 14.8
fatty_acid: 11.7
trans-1,2-CPDA: 10.7
3-Methylbutyryl-CoA: 0.0

hitB

Search NORINE for peptide: strict or relaxed

AMP-binding (26..435): X

NRPSPredictor2: val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DfFHLtgaCK
Nearest Stachelhaus code:: hpg
Stachelhaus code match:: 60% (weak)

hitC

PKS_AT (80..283): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 10.2
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

hitE

Search NORINE for peptide: strict or relaxed

AMP-binding (19..416): X

NRPSPredictor2: val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DcffFaLItK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

hitP2

PKS_AT (557..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.0
inactive: 107.5
Methoxymalonyl-CoA: 66.2
Methylmalonyl-CoA: 62.8
Propionyl-CoA: 47.0
Ethylmalonyl-CoA: 35.6
Isobutyryl-CoA: 30.1
2-Methylbutyryl-CoA: 28.7
Benzoyl-CoA: 26.2
Acetyl-CoA: 26.1
CHC-CoA: 23.7
fatty_acid: 16.7
trans-1,2-CPDA: 16.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (2088..2375): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.3
inactive: 97.6
Methoxymalonyl-CoA: 51.8
Methylmalonyl-CoA: 45.2
Propionyl-CoA: 35.4
Benzoyl-CoA: 24.1
Ethylmalonyl-CoA: 23.2
Isobutyryl-CoA: 22.4
2-Methylbutyryl-CoA: 20.3
Acetyl-CoA: 19.2
fatty_acid: 16.2
CHC-CoA: 10.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

hitP3

PKS_AT (549..836): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.7
inactive: 103.7
Methoxymalonyl-CoA: 57.1
Methylmalonyl-CoA: 54.7
Propionyl-CoA: 41.4
Benzoyl-CoA: 26.3
Ethylmalonyl-CoA: 24.8
2-Methylbutyryl-CoA: 24.3
Isobutyryl-CoA: 22.3
Acetyl-CoA: 18.7
fatty_acid: 14.0
CHC-CoA: 12.5
trans-1,2-CPDA: 8.6
3-Methylbutyryl-CoA: 0.0

hitP4

PKS_AT (574..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.2
inactive: 104.6
Methoxymalonyl-CoA: 49.7
Methylmalonyl-CoA: 46.2
Propionyl-CoA: 34.1
Ethylmalonyl-CoA: 25.4
Isobutyryl-CoA: 24.1
2-Methylbutyryl-CoA: 22.9
Benzoyl-CoA: 22.4
fatty_acid: 22.3
Acetyl-CoA: 20.3
CHC-CoA: 15.2
trans-1,2-CPDA: 9.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (2254..2551): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 213.3
Methylmalonyl-CoA: 212.2
Methoxymalonyl-CoA: 170.9
2-Methylbutyryl-CoA: 142.9
trans-1,2-CPDA: 138.3
Propionyl-CoA: 109.5
CHC-CoA: 95.1
Malonyl-CoA: 94.7
Benzoyl-CoA: 80.7
Isobutyryl-CoA: 74.0
Acetyl-CoA: 52.4
3-Methylbutyryl-CoA: 41.7
fatty_acid: 39.4
inactive: 23.4

PKS_AT (3946..4233): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.6
inactive: 104.5
Methoxymalonyl-CoA: 50.9
Methylmalonyl-CoA: 46.4
Propionyl-CoA: 35.2
Isobutyryl-CoA: 26.6
Ethylmalonyl-CoA: 25.1
2-Methylbutyryl-CoA: 24.4
Benzoyl-CoA: 23.6
fatty_acid: 23.2
Acetyl-CoA: 19.9
CHC-CoA: 13.9
trans-1,2-CPDA: 9.3
3-Methylbutyryl-CoA: 0.0

hitP5

PKS_AT (556..845): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 192.3
inactive: 89.0
Methoxymalonyl-CoA: 64.8
Methylmalonyl-CoA: 52.3
Propionyl-CoA: 34.4
Isobutyryl-CoA: 31.1
Ethylmalonyl-CoA: 28.1
Benzoyl-CoA: 23.6
Acetyl-CoA: 20.2
trans-1,2-CPDA: 14.1
fatty_acid: 13.8
CHC-CoA: 9.6
2-Methylbutyryl-CoA: 0.3
3-Methylbutyryl-CoA: 0.0