BGC0002587 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002587 (Streptomyces ossamyceticus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	112,926	High	ossamycin	PKS

BGC0002587 - Region 1 - T1PKS

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Location: 1 - 112,926 nt. (total: 112,926 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 112,927
Label		BGC0002587

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

ossC: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

ossF: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ossK: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ossL: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ossM: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ossT: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: acylase aminotransferase glycosyltransferase methyltransferase

ossA7: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ossG: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

ossI: methyltransferase

smcogs: SMCOG1248: methyltransferase

ossJ: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

ossQ: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

Hydrolases: esterase

ossA9: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: synthase

ossA1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ossA2: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ossA3: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ossA4: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ossA5: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ossA6: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ossA8: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ossD: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002587.2	1.00	PKS	ossamycin	Streptomyces ossamyceticus
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002587.2	1.00	PKS	ossamycin	Streptomyces ossamyceticus
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 112926:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - ccmal) + (Me-ohmal - redmal) + (mal - pk - ohmal) + (redmal) + (pk) + (emal - ccmal) + (ohmal - pk) + (pk - ccmal)
Non-colinear ordering:: ossA1 + ossA2 + ossA3 + ossA5 + ossA6 + ossA4 + ossA7 + ossA8

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ossA5: mal

PKS_AT (567..848): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 197.5
inactive: 74.8
Methoxymalonyl-CoA: 60.8
Methylmalonyl-CoA: 44.0
Propionyl-CoA: 24.4
Isobutyryl-CoA: 24.4
Acetyl-CoA: 21.4
Benzoyl-CoA: 15.5
CHC-CoA: 6.2
Ethylmalonyl-CoA: 6.0
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ossA6: pk

PKS_AT (576..870): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 215.3
Methylmalonyl-CoA: 209.4
Methoxymalonyl-CoA: 173.5
2-Methylbutyryl-CoA: 138.2
trans-1,2-CPDA: 130.0
Propionyl-CoA: 126.8
CHC-CoA: 108.2
Malonyl-CoA: 101.6
Isobutyryl-CoA: 72.5
Benzoyl-CoA: 61.1
Acetyl-CoA: 57.3
fatty_acid: 37.8
inactive: 36.3
3-Methylbutyryl-CoA: 33.2

ossA1: mmal - mal

PKS_AT (569..871): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.9
Ethylmalonyl-CoA: 193.4
Methoxymalonyl-CoA: 172.7
2-Methylbutyryl-CoA: 136.1
trans-1,2-CPDA: 120.5
Malonyl-CoA: 106.2
Propionyl-CoA: 100.5
Isobutyryl-CoA: 88.3
CHC-CoA: 84.0
Benzoyl-CoA: 63.2
Acetyl-CoA: 57.6
fatty_acid: 36.1
3-Methylbutyryl-CoA: 34.7
inactive: 21.9

PKS_AT (1649..1958): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.0
inactive: 93.0
Methoxymalonyl-CoA: 64.4
Methylmalonyl-CoA: 53.8
Propionyl-CoA: 34.8
Acetyl-CoA: 26.5
Isobutyryl-CoA: 19.2
CHC-CoA: 19.0
Benzoyl-CoA: 18.1
2-Methylbutyryl-CoA: 17.8
Ethylmalonyl-CoA: 16.2
fatty_acid: 15.9
trans-1,2-CPDA: 8.1
3-Methylbutyryl-CoA: 0.0

ossA2: mmal - mal

PKS_AT (561..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.7
Ethylmalonyl-CoA: 203.0
Methoxymalonyl-CoA: 170.9
2-Methylbutyryl-CoA: 137.2
Propionyl-CoA: 112.2
CHC-CoA: 111.6
trans-1,2-CPDA: 109.5
Malonyl-CoA: 97.3
Isobutyryl-CoA: 64.7
Benzoyl-CoA: 54.7
Acetyl-CoA: 30.0
inactive: 28.6
3-Methylbutyryl-CoA: 25.8
fatty_acid: 22.4

PKS_AT (2100..2380): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.0
inactive: 100.3
Methoxymalonyl-CoA: 59.7
Methylmalonyl-CoA: 51.3
Propionyl-CoA: 41.8
Ethylmalonyl-CoA: 25.4
2-Methylbutyryl-CoA: 24.0
Benzoyl-CoA: 21.7
Isobutyryl-CoA: 19.4
fatty_acid: 17.1
Acetyl-CoA: 12.6
trans-1,2-CPDA: 12.3
CHC-CoA: 5.8
3-Methylbutyryl-CoA: 0.0

ossA3: mal - pk - mal

PKS_AT (739..1031): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.5
inactive: 106.7
Methoxymalonyl-CoA: 56.5
Methylmalonyl-CoA: 49.9
Isobutyryl-CoA: 26.7
Propionyl-CoA: 18.4
Ethylmalonyl-CoA: 18.0
Benzoyl-CoA: 16.3
Acetyl-CoA: 15.2
2-Methylbutyryl-CoA: 7.5
CHC-CoA: 7.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1744..2038): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 233.5
Methylmalonyl-CoA: 224.2
Methoxymalonyl-CoA: 176.7
2-Methylbutyryl-CoA: 150.1
CHC-CoA: 136.5
trans-1,2-CPDA: 134.6
Propionyl-CoA: 121.2
Malonyl-CoA: 103.6
Isobutyryl-CoA: 78.4
Acetyl-CoA: 66.5
Benzoyl-CoA: 57.9
3-Methylbutyryl-CoA: 37.2
fatty_acid: 34.1
inactive: 30.8

PKS_AT (3289..3567): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 212.5
inactive: 98.1
Methoxymalonyl-CoA: 58.3
Methylmalonyl-CoA: 44.4
Propionyl-CoA: 27.2
Ethylmalonyl-CoA: 20.8
Acetyl-CoA: 17.8
fatty_acid: 15.4
Benzoyl-CoA: 12.8
Isobutyryl-CoA: 12.3
2-Methylbutyryl-CoA: 6.4
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ossA4: emal - mal

PKS_AT (616..914): emal

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 79.2%; Methylmalonyl-CoA: 79.2%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 229.2
Methylmalonyl-CoA: 202.4
Methoxymalonyl-CoA: 166.9
2-Methylbutyryl-CoA: 147.9
trans-1,2-CPDA: 139.7
Malonyl-CoA: 114.9
Propionyl-CoA: 114.2
CHC-CoA: 106.9
Isobutyryl-CoA: 77.6
Benzoyl-CoA: 72.9
fatty_acid: 48.5
Acetyl-CoA: 41.5
inactive: 40.9
3-Methylbutyryl-CoA: 25.6

PKS_AT (1939..2237): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 206.6
inactive: 108.7
Methoxymalonyl-CoA: 61.0
Methylmalonyl-CoA: 55.5
Propionyl-CoA: 38.2
Isobutyryl-CoA: 29.4
Ethylmalonyl-CoA: 21.7
CHC-CoA: 20.4
fatty_acid: 19.9
Benzoyl-CoA: 19.3
Acetyl-CoA: 15.9
trans-1,2-CPDA: 9.5
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ossA7: mal - pk

PKS_AT (563..839): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.9
inactive: 93.0
Methoxymalonyl-CoA: 58.3
Methylmalonyl-CoA: 51.3
Propionyl-CoA: 33.2
Ethylmalonyl-CoA: 24.8
fatty_acid: 21.6
Isobutyryl-CoA: 17.3
Acetyl-CoA: 16.8
2-Methylbutyryl-CoA: 11.2
CHC-CoA: 6.3
Benzoyl-CoA: 4.4
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1998..2292): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 214.2
Methylmalonyl-CoA: 207.1
Methoxymalonyl-CoA: 164.8
2-Methylbutyryl-CoA: 138.5
trans-1,2-CPDA: 131.7
CHC-CoA: 127.0
Propionyl-CoA: 118.6
Malonyl-CoA: 91.1
Isobutyryl-CoA: 76.2
Benzoyl-CoA: 57.1
Acetyl-CoA: 54.0
inactive: 39.5
3-Methylbutyryl-CoA: 31.9
fatty_acid: 30.2

ossA8: pk - mal

PKS_AT (565..859): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 217.4
Methylmalonyl-CoA: 211.4
Methoxymalonyl-CoA: 166.6
2-Methylbutyryl-CoA: 153.0
Propionyl-CoA: 134.4
CHC-CoA: 132.2
trans-1,2-CPDA: 132.1
Malonyl-CoA: 103.8
Isobutyryl-CoA: 77.7
Acetyl-CoA: 55.7
Benzoyl-CoA: 48.7
fatty_acid: 44.7
inactive: 33.6
3-Methylbutyryl-CoA: 28.4

PKS_AT (2051..2348): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 219.9
inactive: 117.2
Methoxymalonyl-CoA: 75.2
Methylmalonyl-CoA: 67.6
Propionyl-CoA: 58.3
2-Methylbutyryl-CoA: 31.8
Ethylmalonyl-CoA: 28.3
CHC-CoA: 23.7
fatty_acid: 22.2
Isobutyryl-CoA: 19.9
Acetyl-CoA: 12.6
trans-1,2-CPDA: 10.9
Benzoyl-CoA: 10.2
3-Methylbutyryl-CoA: 8.9

Link to PARAS substrate predictor