BGC0000125 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000125 (Streptomyces natalensis)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS-like	1	84,985	natamycin	Polyketide	77%

BGC0000125 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 84,985 nt. (total: 84,985 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 84,986
Label		BGC0000125

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000125.1	1.13	Polyketide	pimaricin	Streptomyces natalensis
BGC0001690.1	0.96	Polyketide	natamycin	Streptomyces gilvosporeus
BGC0000108.1	0.76	Polyketide	natamycin	Streptomyces chattanoogensis
BGC0001068.1	0.57	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0001858.1	0.53	Polyketide	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0001544.1	0.49	NRP, Polyketide	chlorflavonin	Aspergillus campestris IBT 28561
BGC0000016.1	0.49	Other	amphotericin B	Streptomyces nodosus
BGC0001164.1	0.48	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001280.1	0.48	Polyketide	betaenone C, betaenone A	Phoma betae
BGC0001264.1	0.48	Polyketide	betaenone A, betaenone B, betaenone C	Phoma betae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000125.1	0.94	Polyketide	pimaricin	Streptomyces natalensis
BGC0001690.1	0.89	Polyketide	natamycin	Streptomyces gilvosporeus
BGC0000108.1	0.80	Polyketide	natamycin	Streptomyces chattanoogensis
BGC0002031.1	0.65	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0001164.1	0.65	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000160.1	0.65	Polyketide	terreic acid	Aspergillus terreus NIH2624
BGC0000120.1	0.64	Polyketide	patulin	Penicillium expansum
BGC0001276.1	0.64	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001647.1	0.64	Polyketide	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0001273.1	0.64	Polyketide	asperlactone	Aspergillus ochraceus

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 84985:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal - ccmal - ccmal - ccmal) + (X - mal) + (ccmal - ohmal - Me-ohmal - ohmal - ohmal - ohmal) + (ccmal) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

pimS4

PKS_AT (550..835): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 229.7
inactive: 105.0
Methoxymalonyl-CoA: 59.9
Methylmalonyl-CoA: 56.0
Propionyl-CoA: 28.2
Acetyl-CoA: 25.0
Isobutyryl-CoA: 23.9
Benzoyl-CoA: 20.8
Ethylmalonyl-CoA: 15.4
CHC-CoA: 8.5
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

pimS3

PKS_AT (551..836): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 229.0
inactive: 104.2
Methoxymalonyl-CoA: 62.2
Methylmalonyl-CoA: 54.3
Propionyl-CoA: 31.4
Isobutyryl-CoA: 26.7
Acetyl-CoA: 23.6
Ethylmalonyl-CoA: 17.9
Benzoyl-CoA: 17.3
2-Methylbutyryl-CoA: 13.9
fatty_acid: 7.0
CHC-CoA: 5.4
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

pimS2

PKS_AT (561..851): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.7
inactive: 88.5
Methoxymalonyl-CoA: 56.7
Methylmalonyl-CoA: 47.2
Acetyl-CoA: 27.7
Propionyl-CoA: 27.3
Benzoyl-CoA: 23.6
Ethylmalonyl-CoA: 19.2
Isobutyryl-CoA: 18.8
2-Methylbutyryl-CoA: 18.7
CHC-CoA: 16.6
trans-1,2-CPDA: 10.1
fatty_acid: 9.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (2317..2616): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.6
inactive: 85.8
Methoxymalonyl-CoA: 70.3
Methylmalonyl-CoA: 60.0
Isobutyryl-CoA: 33.6
Propionyl-CoA: 29.9
Ethylmalonyl-CoA: 22.6
CHC-CoA: 22.1
Acetyl-CoA: 21.2
Benzoyl-CoA: 19.3
fatty_acid: 13.1
2-Methylbutyryl-CoA: 11.2
trans-1,2-CPDA: 9.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (3860..4157): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 209.6
Ethylmalonyl-CoA: 193.4
Methoxymalonyl-CoA: 165.6
2-Methylbutyryl-CoA: 128.4
trans-1,2-CPDA: 126.0
Propionyl-CoA: 105.8
CHC-CoA: 94.8
Malonyl-CoA: 91.5
Isobutyryl-CoA: 73.1
Benzoyl-CoA: 62.1
Acetyl-CoA: 34.7
fatty_acid: 28.8
3-Methylbutyryl-CoA: 26.6
inactive: 17.4

PKS_AT (5393..5693): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.8
inactive: 98.9
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 61.0
Isobutyryl-CoA: 44.3
Propionyl-CoA: 30.0
Benzoyl-CoA: 19.9
Ethylmalonyl-CoA: 17.4
2-Methylbutyryl-CoA: 17.3
Acetyl-CoA: 15.8
fatty_acid: 13.5
3-Methylbutyryl-CoA: 10.5
CHC-CoA: 8.8
trans-1,2-CPDA: 0.0

PKS_AT (6913..7198): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 223.8
inactive: 100.2
Methoxymalonyl-CoA: 56.7
Methylmalonyl-CoA: 50.6
Isobutyryl-CoA: 27.9
Propionyl-CoA: 23.0
Acetyl-CoA: 22.7
Benzoyl-CoA: 21.3
Ethylmalonyl-CoA: 14.6
fatty_acid: 8.3
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (8430..8723): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 226.6
inactive: 106.3
Methoxymalonyl-CoA: 62.1
Methylmalonyl-CoA: 60.0
Isobutyryl-CoA: 38.2
Propionyl-CoA: 29.9
Benzoyl-CoA: 23.1
Ethylmalonyl-CoA: 22.5
Acetyl-CoA: 20.6
2-Methylbutyryl-CoA: 14.6
CHC-CoA: 11.2
fatty_acid: 10.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

pimS0

Search NORINE for peptide: strict or relaxed

AMP-binding (29..446): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: Gl-frAVtGK
Nearest Stachelhaus code:: hiv-d
Stachelhaus code match:: 50% (weak)

PKS_AT (1209..1511): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 182.5
inactive: 72.9
Methoxymalonyl-CoA: 70.4
Methylmalonyl-CoA: 60.7
Isobutyryl-CoA: 35.1
Ethylmalonyl-CoA: 33.6
Propionyl-CoA: 32.9
Acetyl-CoA: 29.9
fatty_acid: 25.2
Benzoyl-CoA: 24.0
CHC-CoA: 23.1
2-Methylbutyryl-CoA: 15.3
trans-1,2-CPDA: 15.1
3-Methylbutyryl-CoA: 0.0

pimS1

PKS_AT (565..864): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.1
inactive: 101.9
Methoxymalonyl-CoA: 63.8
Methylmalonyl-CoA: 54.0
Isobutyryl-CoA: 46.0
Propionyl-CoA: 31.7
Benzoyl-CoA: 22.7
Acetyl-CoA: 19.9
Ethylmalonyl-CoA: 19.3
2-Methylbutyryl-CoA: 14.8
trans-1,2-CPDA: 12.6
CHC-CoA: 7.3
3-Methylbutyryl-CoA: 6.9
fatty_acid: 0.0

PKS_AT (2103..2393): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.5
inactive: 101.6
Methoxymalonyl-CoA: 51.1
Methylmalonyl-CoA: 42.0
Acetyl-CoA: 19.8
Propionyl-CoA: 18.9
Benzoyl-CoA: 15.5
2-Methylbutyryl-CoA: 11.5
fatty_acid: 7.7
CHC-CoA: 5.1
Isobutyryl-CoA: 4.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -3.9

PKS_AT (3829..4119): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.7
inactive: 108.0
Methoxymalonyl-CoA: 51.3
Methylmalonyl-CoA: 38.8
Propionyl-CoA: 28.9
Acetyl-CoA: 22.0
Benzoyl-CoA: 16.2
Ethylmalonyl-CoA: 12.9
fatty_acid: 11.7
Isobutyryl-CoA: 11.0
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.6

PKS_AT (5543..5833): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.8
inactive: 108.1
Methoxymalonyl-CoA: 63.1
Methylmalonyl-CoA: 47.4
Propionyl-CoA: 27.6
Acetyl-CoA: 24.7
Ethylmalonyl-CoA: 21.0
2-Methylbutyryl-CoA: 18.1
Benzoyl-CoA: 17.6
fatty_acid: 17.4
Isobutyryl-CoA: 13.1
CHC-CoA: 10.4
trans-1,2-CPDA: 6.9
3-Methylbutyryl-CoA: 0.0