BGC0001288 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001288 (Micromonospora sp. GMKU326)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,PKS-like	1	149,333	chlorothricin / deschlorothricin	Polyketide:Modular type I + Polyketide:Iterative type I + Saccharide:Oligosaccharide	46%

BGC0001288 - Region 1 - PKS-like,T1PKS

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Location: 1 - 149,333 nt. (total: 149,333 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
PKS-like: (t2pks2 or ksIII)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 149,334
Label		BGC0001288

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001288.1	1.59	Polyketide	maklamicin	Micromonospora sp. GMKU326
BGC0001909.1	1.11	Polyketide	strobilurin	Strobilurus tenacellus
BGC0001858.1	1.09	Polyketide	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0001280.1	1.05	Polyketide	betaenone C, betaenone A	Phoma betae
BGC0001264.1	1.05	Polyketide	betaenone A, betaenone B, betaenone C	Phoma betae
BGC0001276.1	1.05	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.1	1.05	Polyketide	asperlactone	Aspergillus ochraceus
BGC0000041.1	1.04	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001275.1	1.03	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.1	1.03	Polyketide	(-)-Mellein	Parastagonospora nodorum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001288.1	0.95	Polyketide	maklamicin	Micromonospora sp. GMKU326
BGC0001276.1	0.68	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.1	0.68	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001275.1	0.68	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.1	0.67	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0000160.1	0.66	Polyketide	terreic acid	Aspergillus terreus NIH2624
BGC0000120.1	0.66	Polyketide	patulin	Penicillium expansum
BGC0000170.1	0.66	Polyketide	yanuthone D	Aspergillus niger ATCC 1015
BGC0001909.1	0.66	Polyketide	strobilurin	Strobilurus tenacellus
BGC0000041.1	0.65	Polyketide	oronofacic acid	Pseudomonas syringae

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 198 - 149333:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-ccmal - ccmal - Me-ccmal - Me-ccmal - ccmal) + (Me-ccmal - ccmal - ccmal - Me-ccmal) + (Me-redmal - Me-ccmal) + (mal) + (Me-ccmal - ccmal - ccmal - Me-redmal) + (redmal - Me-ccmal) + (pk)
Non-colinear ordering:: makA1 + BAQ25481.1 + BAQ25482.1 + makA4 + makA2 + makA3 + BAQ25507.1

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 198 - 62767:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal - ccmal - ccmal - Me-ccmal) + (Me-redmal - Me-ccmal) + (mal)
Non-colinear ordering:: BAQ25481.1 + BAQ25482.1 + makA4

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 40807 - 69300:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 48259 - 89300:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-ccmal - ccmal - Me-ccmal - Me-ccmal - ccmal) + (pk)
Non-colinear ordering:: makA1 + BAQ25507.1

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 49341 - 149333:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-ccmal - ccmal - Me-ccmal - Me-ccmal - ccmal) + (Me-ccmal - ccmal - ccmal - Me-redmal) + (redmal - Me-ccmal) + (pk)
Non-colinear ordering:: makA1 + makA2 + makA3 + BAQ25507.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

BAQ25481.1

PKS_AT (658..953): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 79.2%; Ethylmalonyl-CoA: 70.8%; Methoxymalonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 166.8
Methoxymalonyl-CoA: 136.4
Ethylmalonyl-CoA: 127.5
2-Methylbutyryl-CoA: 108.4
trans-1,2-CPDA: 105.0
CHC-CoA: 99.2
Propionyl-CoA: 96.3
Malonyl-CoA: 88.6
Isobutyryl-CoA: 77.6
Benzoyl-CoA: 55.2
fatty_acid: 53.4
3-Methylbutyryl-CoA: 38.7
Acetyl-CoA: 32.8
inactive: 16.6

PKS_AT (2439..2729): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 171.5
inactive: 91.3
Methoxymalonyl-CoA: 55.1
Methylmalonyl-CoA: 45.0
Propionyl-CoA: 28.4
Ethylmalonyl-CoA: 22.7
fatty_acid: 20.3
Isobutyryl-CoA: 19.3
Acetyl-CoA: 18.0
Benzoyl-CoA: 11.9
CHC-CoA: 9.4
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (4159..4446): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.6
inactive: 83.9
Methoxymalonyl-CoA: 53.6
Methylmalonyl-CoA: 40.9
Propionyl-CoA: 28.9
Ethylmalonyl-CoA: 22.2
fatty_acid: 18.7
Isobutyryl-CoA: 17.9
Acetyl-CoA: 17.6
Benzoyl-CoA: 9.4
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (5883..6226): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.5
Ethylmalonyl-CoA: 187.7
Methoxymalonyl-CoA: 169.0
2-Methylbutyryl-CoA: 141.2
Propionyl-CoA: 125.2
trans-1,2-CPDA: 113.5
CHC-CoA: 105.5
Malonyl-CoA: 98.3
Isobutyryl-CoA: 64.9
Benzoyl-CoA: 49.7
inactive: 30.9
fatty_acid: 30.8
3-Methylbutyryl-CoA: 29.4
Acetyl-CoA: 23.2

BAQ25482.1

PKS_AT (582..920): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 189.7
Ethylmalonyl-CoA: 174.6
Methoxymalonyl-CoA: 165.0
2-Methylbutyryl-CoA: 137.5
Propionyl-CoA: 121.2
trans-1,2-CPDA: 118.4
CHC-CoA: 109.7
Malonyl-CoA: 97.0
Isobutyryl-CoA: 58.2
Benzoyl-CoA: 50.1
inactive: 32.6
fatty_acid: 31.6
3-Methylbutyryl-CoA: 23.6
Acetyl-CoA: 21.3

PKS_AT (2673..3018): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 193.3
Ethylmalonyl-CoA: 183.5
Methoxymalonyl-CoA: 166.8
2-Methylbutyryl-CoA: 135.4
Propionyl-CoA: 120.9
trans-1,2-CPDA: 117.7
CHC-CoA: 113.8
Malonyl-CoA: 99.9
Isobutyryl-CoA: 61.7
Benzoyl-CoA: 51.5
inactive: 33.4
fatty_acid: 32.1
Acetyl-CoA: 25.4
3-Methylbutyryl-CoA: 24.7

makA4

PKS_AT (572..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 183.2
inactive: 83.2
Methoxymalonyl-CoA: 70.3
Methylmalonyl-CoA: 48.0
Propionyl-CoA: 34.1
Acetyl-CoA: 21.0
Isobutyryl-CoA: 17.9
fatty_acid: 17.7
Ethylmalonyl-CoA: 15.0
Benzoyl-CoA: 14.3
2-Methylbutyryl-CoA: 11.7
CHC-CoA: 10.2
trans-1,2-CPDA: 8.3
3-Methylbutyryl-CoA: 0.0

BAQ25507.1

PKS_AT (543..833): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; Methylmalonyl-CoA: 70.8%; Propionyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 126.1
Methoxymalonyl-CoA: 100.3
Ethylmalonyl-CoA: 98.3
Malonyl-CoA: 73.7
trans-1,2-CPDA: 64.1
Isobutyryl-CoA: 63.8
Propionyl-CoA: 62.7
2-Methylbutyryl-CoA: 51.2
Acetyl-CoA: 42.7
Benzoyl-CoA: 38.3
fatty_acid: 37.7
3-Methylbutyryl-CoA: 34.8
CHC-CoA: 26.4
inactive: 11.7

makA1

PKS_AT (575..871): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 215.4
inactive: 112.5
Methoxymalonyl-CoA: 64.0
Methylmalonyl-CoA: 55.0
Propionyl-CoA: 33.0
Isobutyryl-CoA: 27.4
Acetyl-CoA: 25.4
Benzoyl-CoA: 19.5
trans-1,2-CPDA: 12.3
CHC-CoA: 11.8
fatty_acid: 8.0
2-Methylbutyryl-CoA: 7.4
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -3.5

PKS_AT (1613..1960): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.1
Ethylmalonyl-CoA: 177.8
Methoxymalonyl-CoA: 165.2
2-Methylbutyryl-CoA: 133.4
Propionyl-CoA: 117.4
CHC-CoA: 110.4
trans-1,2-CPDA: 108.8
Malonyl-CoA: 96.0
Isobutyryl-CoA: 60.4
Benzoyl-CoA: 51.8
inactive: 35.1
fatty_acid: 33.6
3-Methylbutyryl-CoA: 24.8
Acetyl-CoA: 23.0

PKS_AT (3387..3677): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 195.4
inactive: 85.4
Methoxymalonyl-CoA: 59.3
Methylmalonyl-CoA: 48.8
Propionyl-CoA: 26.6
Isobutyryl-CoA: 21.4
Acetyl-CoA: 14.8
fatty_acid: 10.9
Ethylmalonyl-CoA: 8.6
Benzoyl-CoA: 8.0
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (5170..5513): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 189.2
Ethylmalonyl-CoA: 174.3
Methoxymalonyl-CoA: 160.0
2-Methylbutyryl-CoA: 133.2
Propionyl-CoA: 119.9
CHC-CoA: 111.6
trans-1,2-CPDA: 109.7
Malonyl-CoA: 96.8
Isobutyryl-CoA: 56.4
Benzoyl-CoA: 47.6
inactive: 35.6
fatty_acid: 34.9
Acetyl-CoA: 21.4
3-Methylbutyryl-CoA: 18.8

PKS_AT (6930..7273): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 192.2
Ethylmalonyl-CoA: 181.3
Methoxymalonyl-CoA: 160.7
2-Methylbutyryl-CoA: 134.8
Propionyl-CoA: 121.5
trans-1,2-CPDA: 110.6
CHC-CoA: 110.2
Malonyl-CoA: 100.2
Isobutyryl-CoA: 59.5
Benzoyl-CoA: 47.9
inactive: 31.4
fatty_acid: 28.7
Acetyl-CoA: 24.1
3-Methylbutyryl-CoA: 22.9

PKS_AT (8702..8992): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.8
inactive: 90.2
Methoxymalonyl-CoA: 56.5
Methylmalonyl-CoA: 42.6
Propionyl-CoA: 27.2
Isobutyryl-CoA: 21.5
Ethylmalonyl-CoA: 18.1
fatty_acid: 17.6
Acetyl-CoA: 14.8
Benzoyl-CoA: 9.0
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

makA2

PKS_AT (635..930): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 79.2%; Ethylmalonyl-CoA: 70.8%; Methoxymalonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 165.5
Methoxymalonyl-CoA: 135.6
Ethylmalonyl-CoA: 128.1
2-Methylbutyryl-CoA: 109.5
trans-1,2-CPDA: 106.0
CHC-CoA: 100.5
Propionyl-CoA: 97.0
Malonyl-CoA: 84.8
Isobutyryl-CoA: 78.6
Benzoyl-CoA: 55.4
fatty_acid: 53.4
3-Methylbutyryl-CoA: 38.9
Acetyl-CoA: 32.8
inactive: 16.5

PKS_AT (2355..2638): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 171.3
inactive: 86.3
Methoxymalonyl-CoA: 56.9
Methylmalonyl-CoA: 41.3
Propionyl-CoA: 25.1
Ethylmalonyl-CoA: 18.6
Acetyl-CoA: 18.6
Isobutyryl-CoA: 14.1
Benzoyl-CoA: 9.4
trans-1,2-CPDA: 0.0
fatty_acid: -0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (4087..4377): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.4
inactive: 88.6
Methoxymalonyl-CoA: 54.9
Methylmalonyl-CoA: 44.8
Propionyl-CoA: 20.9
fatty_acid: 18.9
Acetyl-CoA: 18.7
Isobutyryl-CoA: 17.0
Ethylmalonyl-CoA: 15.8
Benzoyl-CoA: 10.8
2-Methylbutyryl-CoA: 1.0
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (5841..6133): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 190.9
Ethylmalonyl-CoA: 178.2
Methoxymalonyl-CoA: 161.1
2-Methylbutyryl-CoA: 133.1
Propionyl-CoA: 121.3
CHC-CoA: 109.8
trans-1,2-CPDA: 108.2
Malonyl-CoA: 97.1
Isobutyryl-CoA: 57.8
Benzoyl-CoA: 51.4
inactive: 32.2
fatty_acid: 28.9
3-Methylbutyryl-CoA: 27.3
Acetyl-CoA: 25.6

makA3

PKS_AT (586..876): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 169.8
inactive: 83.1
Methoxymalonyl-CoA: 54.3
Methylmalonyl-CoA: 44.3
Ethylmalonyl-CoA: 28.1
Propionyl-CoA: 26.8
Acetyl-CoA: 18.6
Isobutyryl-CoA: 15.4
Benzoyl-CoA: 13.7
2-Methylbutyryl-CoA: 9.5
trans-1,2-CPDA: 8.9
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
fatty_acid: -2.1

PKS_AT (2646..2940): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.4
Ethylmalonyl-CoA: 180.7
Methoxymalonyl-CoA: 163.8
2-Methylbutyryl-CoA: 136.6
Propionyl-CoA: 121.7
CHC-CoA: 107.7
trans-1,2-CPDA: 107.5
Malonyl-CoA: 98.8
Isobutyryl-CoA: 58.3
Benzoyl-CoA: 51.9
inactive: 32.3
3-Methylbutyryl-CoA: 28.1
fatty_acid: 27.5
Acetyl-CoA: 24.5

makD1

Search NORINE for peptide: strict or relaxed

AMP-binding (10..370): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: vHlflsrITK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 40% (weak)