BGC0001477 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001477 (Streptomyces sp. ID38640)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	116,700	sceliphrolactam	Polyketide	40%

BGC0001477 - Region 1 - NRPS,T1PKS

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Location: 1 - 116,700 nt. (total: 116,700 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 116,701
Label		BGC0001477

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001477.1	1.05	NRP, Polyketide	lydicamycin	Streptomyces sp. ID38640
BGC0000041.1	0.62	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0002031.1	0.58	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0001858.1	0.56	Polyketide	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0001068.1	0.56	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0000457.1	0.55	NRP	vicibactin	Rhizobium etli CFN 42
BGC0000353.1	0.55	NRP	tacrolimus	Streptomyces sp. MA6548
BGC0001050.1	0.53	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0000067.1	0.51	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001873.1	0.51	NRP	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001477.1	0.90	NRP, Polyketide	lydicamycin	Streptomyces sp. ID38640
BGC0000041.1	0.67	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0002031.1	0.65	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0000067.1	0.65	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000353.1	0.64	NRP	tacrolimus	Streptomyces sp. MA6548
BGC0001273.1	0.63	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276.1	0.63	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.1	0.62	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.1	0.62	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0000457.1	0.62	NRP	vicibactin	Rhizobium etli CFN 42

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 116700:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - Me-ccmal) + (ccmal - ccmal - Me-ccmal - ccmal) + (ccmal - Me-ccmal) + (Me-ccmal) + (redmal - Me-ccmal - ccmal) + (ohmal - redmal) + (Me-ccmal - ohmal) + (gly) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

AVV61977.1

PKS_AT (61..299): pk

ATSignature: (unknown)

No matches above 50%

minowa: trans-1,2-CPDA

Prediction, score:

trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

AVV61979.1

PKS_AT (571..868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 212.4
inactive: 117.5
Methoxymalonyl-CoA: 51.7
Methylmalonyl-CoA: 46.3
Propionyl-CoA: 42.2
Isobutyryl-CoA: 21.4
Acetyl-CoA: 17.3
Ethylmalonyl-CoA: 15.1
CHC-CoA: 11.0
Benzoyl-CoA: 9.1
2-Methylbutyryl-CoA: 5.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2321..2616): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.9
Ethylmalonyl-CoA: 206.7
Methoxymalonyl-CoA: 176.5
trans-1,2-CPDA: 129.6
2-Methylbutyryl-CoA: 129.5
CHC-CoA: 116.3
Propionyl-CoA: 112.0
Malonyl-CoA: 105.6
Isobutyryl-CoA: 76.0
Benzoyl-CoA: 63.2
Acetyl-CoA: 57.1
3-Methylbutyryl-CoA: 43.8
fatty_acid: 42.7
inactive: 40.5

AVV61980.1

PKS_AT (561..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 219.6
inactive: 114.8
Methoxymalonyl-CoA: 54.4
Methylmalonyl-CoA: 50.3
Propionyl-CoA: 41.5
Acetyl-CoA: 18.3
Isobutyryl-CoA: 17.0
2-Methylbutyryl-CoA: 13.9
Ethylmalonyl-CoA: 11.3
fatty_acid: 9.4
Benzoyl-CoA: 7.8
CHC-CoA: 5.3
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2295..2588): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.7
inactive: 111.1
Methoxymalonyl-CoA: 54.8
Methylmalonyl-CoA: 49.2
Propionyl-CoA: 41.6
Acetyl-CoA: 19.9
Isobutyryl-CoA: 19.6
Ethylmalonyl-CoA: 12.2
2-Methylbutyryl-CoA: 9.8
CHC-CoA: 8.9
fatty_acid: 8.6
trans-1,2-CPDA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4033..4330): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.9
Ethylmalonyl-CoA: 209.8
Methoxymalonyl-CoA: 175.9
trans-1,2-CPDA: 130.4
2-Methylbutyryl-CoA: 125.5
Propionyl-CoA: 107.5
CHC-CoA: 106.9
Malonyl-CoA: 103.0
Isobutyryl-CoA: 77.4
Benzoyl-CoA: 63.6
Acetyl-CoA: 58.3
inactive: 43.0
3-Methylbutyryl-CoA: 41.3
fatty_acid: 40.4

PKS_AT (5785..6086): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 221.8
inactive: 114.2
Methoxymalonyl-CoA: 56.5
Methylmalonyl-CoA: 55.3
Propionyl-CoA: 40.3
Isobutyryl-CoA: 22.7
Acetyl-CoA: 17.8
Ethylmalonyl-CoA: 16.9
fatty_acid: 12.4
2-Methylbutyryl-CoA: 10.3
CHC-CoA: 9.3
Benzoyl-CoA: 7.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

AVV61981.1

PKS_AT (589..880): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 212.5
inactive: 115.5
Methoxymalonyl-CoA: 61.2
Methylmalonyl-CoA: 55.6
Propionyl-CoA: 42.8
Isobutyryl-CoA: 25.8
Acetyl-CoA: 19.6
2-Methylbutyryl-CoA: 12.7
Ethylmalonyl-CoA: 10.9
fatty_acid: 9.4
Benzoyl-CoA: 8.4
CHC-CoA: 7.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2295..2590): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.9
Ethylmalonyl-CoA: 206.7
Methoxymalonyl-CoA: 176.5
trans-1,2-CPDA: 129.6
2-Methylbutyryl-CoA: 129.5
CHC-CoA: 116.3
Propionyl-CoA: 112.0
Malonyl-CoA: 105.6
Isobutyryl-CoA: 76.0
Benzoyl-CoA: 63.2
Acetyl-CoA: 57.1
3-Methylbutyryl-CoA: 43.8
fatty_acid: 42.7
inactive: 40.5

AVV61982.1

PKS_AT (576..871): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.2
Ethylmalonyl-CoA: 207.6
Methoxymalonyl-CoA: 179.7
2-Methylbutyryl-CoA: 137.4
trans-1,2-CPDA: 128.5
CHC-CoA: 117.6
Propionyl-CoA: 113.4
Malonyl-CoA: 106.1
Isobutyryl-CoA: 75.3
Benzoyl-CoA: 61.4
Acetyl-CoA: 52.3
inactive: 44.2
fatty_acid: 42.9
3-Methylbutyryl-CoA: 42.5

AVV61983.1

PKS_AT (579..869): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.2
inactive: 113.5
Methoxymalonyl-CoA: 55.2
Methylmalonyl-CoA: 48.3
Propionyl-CoA: 34.6
Acetyl-CoA: 24.2
Isobutyryl-CoA: 13.8
2-Methylbutyryl-CoA: 12.9
Benzoyl-CoA: 12.4
CHC-CoA: 12.0
fatty_acid: 11.6
Ethylmalonyl-CoA: 9.9
trans-1,2-CPDA: 5.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (2674..2969): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.2
Ethylmalonyl-CoA: 207.6
Methoxymalonyl-CoA: 179.7
2-Methylbutyryl-CoA: 137.4
trans-1,2-CPDA: 128.5
CHC-CoA: 117.6
Propionyl-CoA: 113.4
Malonyl-CoA: 106.1
Isobutyryl-CoA: 75.3
Benzoyl-CoA: 61.4
Acetyl-CoA: 52.3
inactive: 44.2
fatty_acid: 42.9
3-Methylbutyryl-CoA: 42.5

PKS_AT (4427..4725): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 222.2
inactive: 113.6
Methoxymalonyl-CoA: 57.2
Methylmalonyl-CoA: 54.6
Propionyl-CoA: 40.7
Isobutyryl-CoA: 22.6
Acetyl-CoA: 18.0
Ethylmalonyl-CoA: 16.6
fatty_acid: 12.6
2-Methylbutyryl-CoA: 11.2
CHC-CoA: 9.1
Benzoyl-CoA: 7.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

AVV61984.1

PKS_AT (552..845): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.6
inactive: 112.0
Methoxymalonyl-CoA: 53.8
Methylmalonyl-CoA: 42.5
Propionyl-CoA: 40.6
Acetyl-CoA: 23.1
Isobutyryl-CoA: 20.3
2-Methylbutyryl-CoA: 13.3
Benzoyl-CoA: 8.3
Ethylmalonyl-CoA: 6.7
CHC-CoA: 6.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2053..2352): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.8
inactive: 104.2
Methoxymalonyl-CoA: 65.9
Methylmalonyl-CoA: 47.7
Isobutyryl-CoA: 30.8
Propionyl-CoA: 30.7
Acetyl-CoA: 22.5
Ethylmalonyl-CoA: 12.0
Benzoyl-CoA: 10.6
CHC-CoA: 4.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.7

AVV61985.1

PKS_AT (573..870): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.1
Ethylmalonyl-CoA: 202.5
Methoxymalonyl-CoA: 174.5
2-Methylbutyryl-CoA: 144.6
Propionyl-CoA: 127.1
trans-1,2-CPDA: 118.7
CHC-CoA: 105.8
Malonyl-CoA: 92.2
Isobutyryl-CoA: 78.8
Benzoyl-CoA: 72.2
fatty_acid: 42.8
Acetyl-CoA: 38.4
3-Methylbutyryl-CoA: 37.3
inactive: 24.3

PKS_AT (2328..2621): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 201.0
inactive: 102.0
Methoxymalonyl-CoA: 49.7
Methylmalonyl-CoA: 44.5
Propionyl-CoA: 35.0
Acetyl-CoA: 21.7
2-Methylbutyryl-CoA: 19.3
Isobutyryl-CoA: 15.9
Ethylmalonyl-CoA: 9.5
Benzoyl-CoA: 7.7
fatty_acid: 6.8
CHC-CoA: 2.9
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

AVV61987.1

Search NORINE for peptide: strict or relaxed

AMP-binding (458..843): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

AVV61989.1

PKS_AT (655..955): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.4
inactive: 117.1
Methoxymalonyl-CoA: 67.6
Methylmalonyl-CoA: 49.8
Isobutyryl-CoA: 28.0
Ethylmalonyl-CoA: 27.5
Propionyl-CoA: 25.7
Acetyl-CoA: 23.7
Benzoyl-CoA: 14.0
2-Methylbutyryl-CoA: 10.4
CHC-CoA: 7.5
trans-1,2-CPDA: 7.1
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0