BGC0000074 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000074 (Streptomyces hygroscopicus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS-like	1	86,941	High	herbimycin A	PKS

BGC0000074 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 86,941 nt. (total: 86,941 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 86,942
Label		BGC0000074

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

AAY28212.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

hbmI: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

hbmK: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

hbmL: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

hbmP: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acetyltransferase

hbmAII: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

Hydrolases: hydrolase phosphatase

AAY28213.1: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

AAY28220.1: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

hbmH: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

Ligases: synthase

hbmAI: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

hbmAIII: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

hbmO: 3-dehydroquinate synthase

smcogs: SMCOG1183: 3-dehydroquinate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000074.5	1.43	PKS	herbimycin A	Streptomyces hygroscopicus
BGC0001275.3	0.89	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000066.5	0.88	PKS	geldanamycin	Streptomyces hygroscopicus
BGC0000067.5	0.87	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000041.3	0.78	PKS	oronofacic acid	Pseudomonas syringae
BGC0002191.2	0.65	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0000068.5	0.65	PKS	geldanamycin	Streptomyces hygroscopicus
BGC0002192.3	0.61	PKS	FR901512	fungal sp. No.14919
BGC0001268.4	0.58	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001051.5	0.42	NRPS, PKS	thuggacin A	Sorangium cellulosum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000074.5	1.00	PKS	herbimycin A	Streptomyces hygroscopicus
BGC0000066.5	0.83	PKS	geldanamycin	Streptomyces hygroscopicus
BGC0000067.5	0.81	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000068.5	0.78	PKS	geldanamycin	Streptomyces hygroscopicus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.71	PKS	oronofacic acid	Pseudomonas syringae
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.63	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 7676 - 86941:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (AHBA - Me-redmal - redmxmal - Me-ohmal) + (Me-ccmal - ohmxmal) + (redmal - Me-ccmal)
Non-colinear ordering:: hbmAI + hbmAII + hbmAIII

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

hbmAI: AHBA - mmal - mxmal - mmal

CAL_domain (34..442): AHBA

minowa: AHBA

Prediction, score:

AHBA: 539.1
shikimic_acid: 269.8
Acetyl-CoA: 12.6
fatty_acid: 0.0
NH2: 0.0

PKS_AT (1628..1925): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 203.1
Ethylmalonyl-CoA: 182.4
Methoxymalonyl-CoA: 164.1
2-Methylbutyryl-CoA: 115.7
Malonyl-CoA: 106.2
Propionyl-CoA: 104.6
trans-1,2-CPDA: 95.0
CHC-CoA: 78.8
Isobutyryl-CoA: 69.1
Benzoyl-CoA: 59.8
Acetyl-CoA: 45.5
3-Methylbutyryl-CoA: 37.0
fatty_acid: 25.5
inactive: 23.1

PKS_AT (3709..4009): mxmal

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 91.7%; Methylmalonyl-CoA: 91.7%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 200.8
Methylmalonyl-CoA: 192.2
Ethylmalonyl-CoA: 188.2
2-Methylbutyryl-CoA: 134.8
trans-1,2-CPDA: 105.5
Malonyl-CoA: 102.5
Propionyl-CoA: 95.4
CHC-CoA: 84.7
Isobutyryl-CoA: 61.6
Benzoyl-CoA: 53.6
Acetyl-CoA: 41.7
3-Methylbutyryl-CoA: 40.6
inactive: 28.0
fatty_acid: 14.7

PKS_AT (5759..6058): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 203.7
Ethylmalonyl-CoA: 182.0
Methoxymalonyl-CoA: 166.0
2-Methylbutyryl-CoA: 112.9
Malonyl-CoA: 105.9
Propionyl-CoA: 98.1
trans-1,2-CPDA: 94.3
CHC-CoA: 72.1
Isobutyryl-CoA: 65.6
Benzoyl-CoA: 58.3
Acetyl-CoA: 42.2
3-Methylbutyryl-CoA: 34.8
inactive: 21.3
fatty_acid: 21.2

hbmAII: mmal - mxmal

PKS_AT (574..873): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.3
Ethylmalonyl-CoA: 183.2
Methoxymalonyl-CoA: 166.4
2-Methylbutyryl-CoA: 114.3
Malonyl-CoA: 107.4
Propionyl-CoA: 99.1
trans-1,2-CPDA: 95.3
CHC-CoA: 72.4
Isobutyryl-CoA: 66.4
Benzoyl-CoA: 58.7
Acetyl-CoA: 42.3
3-Methylbutyryl-CoA: 34.7
inactive: 21.6
fatty_acid: 21.2

PKS_AT (2348..2648): mxmal

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 91.7%; Methylmalonyl-CoA: 91.7%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 202.2
Methylmalonyl-CoA: 192.2
Ethylmalonyl-CoA: 186.9
2-Methylbutyryl-CoA: 133.8
trans-1,2-CPDA: 109.2
Malonyl-CoA: 101.1
Propionyl-CoA: 94.5
CHC-CoA: 88.3
Isobutyryl-CoA: 61.5
Benzoyl-CoA: 51.2
Acetyl-CoA: 41.0
3-Methylbutyryl-CoA: 36.3
inactive: 27.5
fatty_acid: 17.9

hbmAIII: mal - mmal

PKS_AT (583..885): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 235.0
inactive: 125.8
Methoxymalonyl-CoA: 71.2
Methylmalonyl-CoA: 64.5
Propionyl-CoA: 38.8
Isobutyryl-CoA: 33.1
Benzoyl-CoA: 30.6
Ethylmalonyl-CoA: 21.2
Acetyl-CoA: 19.2
2-Methylbutyryl-CoA: 15.0
trans-1,2-CPDA: 14.3
CHC-CoA: 13.8
fatty_acid: 10.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (2665..2962): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.7
Ethylmalonyl-CoA: 182.6
Methoxymalonyl-CoA: 164.7
2-Methylbutyryl-CoA: 118.9
Malonyl-CoA: 105.3
Propionyl-CoA: 105.0
trans-1,2-CPDA: 95.5
CHC-CoA: 78.9
Isobutyryl-CoA: 68.6
Benzoyl-CoA: 60.1
Acetyl-CoA: 45.4
3-Methylbutyryl-CoA: 36.3
fatty_acid: 25.6
inactive: 23.6

Link to PARAS substrate predictor