BGC0000074 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000074 (Streptomyces hygroscopicus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS-like	1	86,941	herbimycin A	PKS	100%

BGC0000074 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 86,941 nt. (total: 86,941 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 86,942
Label		BGC0000074

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000074	1.45	Polyketide	herbimycin A	Streptomyces hygroscopicus
BGC0000066	0.90	Polyketide	geldanamycin	Streptomyces hygroscopicus
BGC0000067	0.86	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000041	0.78	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000068	0.65	Polyketide	geldanamycin	Streptomyces hygroscopicus
BGC0001163	0.62	Polyketide	1-heptadecene	Moorea producens 3L
BGC0002476	0.59	NRP	enterobactin	Escherichia coli str. K-12 substr. MG1655
BGC0001275	0.57	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001913	0.57	Polyketide	phomoidride	fungal sp. ATCC 74256
BGC0001068	0.56	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000074	1.00	Polyketide	herbimycin A	Streptomyces hygroscopicus
BGC0000066	0.83	Polyketide	geldanamycin	Streptomyces hygroscopicus
BGC0000067	0.81	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000068	0.78	Polyketide	geldanamycin	Streptomyces hygroscopicus
BGC0000041	0.71	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000844	0.68	Other	clavulanic acid	Streptomyces clavuligerus
BGC0000892	0.67	Other	caryoynencin	Burkholderia gladioli BSR3
BGC0002476	0.67	NRP	enterobactin	Escherichia coli str. K-12 substr. MG1655
BGC0001374	0.66	Other	thienodolin	Streptomyces albogriseolus
BGC0002631	0.66	RiPP	myxarylin	Pyxidicoccus fallax

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 7676 - 86941:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal - redmxmal - Me-ohmal) + (Me-ccmal - ohmxmal) + (redmal - Me-ccmal)
Non-colinear ordering:: hbmAI + hbmAII + hbmAIII

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

hbmAI

CAL_domain (34..442): pk

minowa: AHBA

Prediction, score:

AHBA: 539.1
shikimic_acid: 269.8
Acetyl-CoA: 12.6
fatty_acid: 0.0
NH2: 0.0

PKS_AT (1628..1925): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 203.1
Ethylmalonyl-CoA: 182.4
Methoxymalonyl-CoA: 164.1
2-Methylbutyryl-CoA: 115.7
Malonyl-CoA: 106.2
Propionyl-CoA: 104.6
trans-1,2-CPDA: 95.0
CHC-CoA: 78.8
Isobutyryl-CoA: 69.1
Benzoyl-CoA: 59.8
Acetyl-CoA: 45.5
3-Methylbutyryl-CoA: 37.0
fatty_acid: 25.5
inactive: 23.1

PKS_AT (3709..4009): mxmal

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 91.7%; Methylmalonyl-CoA: 91.7%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 200.8
Methylmalonyl-CoA: 192.2
Ethylmalonyl-CoA: 188.2
2-Methylbutyryl-CoA: 134.8
trans-1,2-CPDA: 105.5
Malonyl-CoA: 102.5
Propionyl-CoA: 95.4
CHC-CoA: 84.7
Isobutyryl-CoA: 61.6
Benzoyl-CoA: 53.6
Acetyl-CoA: 41.7
3-Methylbutyryl-CoA: 40.6
inactive: 28.0
fatty_acid: 14.7

PKS_AT (5759..6058): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 203.7
Ethylmalonyl-CoA: 182.0
Methoxymalonyl-CoA: 166.0
2-Methylbutyryl-CoA: 112.9
Malonyl-CoA: 105.9
Propionyl-CoA: 98.1
trans-1,2-CPDA: 94.3
CHC-CoA: 72.1
Isobutyryl-CoA: 65.6
Benzoyl-CoA: 58.3
Acetyl-CoA: 42.2
3-Methylbutyryl-CoA: 34.8
inactive: 21.3
fatty_acid: 21.2

hbmAII

PKS_AT (574..873): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.3
Ethylmalonyl-CoA: 183.2
Methoxymalonyl-CoA: 166.4
2-Methylbutyryl-CoA: 114.3
Malonyl-CoA: 107.4
Propionyl-CoA: 99.1
trans-1,2-CPDA: 95.3
CHC-CoA: 72.4
Isobutyryl-CoA: 66.4
Benzoyl-CoA: 58.7
Acetyl-CoA: 42.3
3-Methylbutyryl-CoA: 34.7
inactive: 21.6
fatty_acid: 21.2

PKS_AT (2348..2648): mxmal

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 91.7%; Methylmalonyl-CoA: 91.7%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 202.2
Methylmalonyl-CoA: 192.2
Ethylmalonyl-CoA: 186.9
2-Methylbutyryl-CoA: 133.8
trans-1,2-CPDA: 109.2
Malonyl-CoA: 101.1
Propionyl-CoA: 94.5
CHC-CoA: 88.3
Isobutyryl-CoA: 61.5
Benzoyl-CoA: 51.2
Acetyl-CoA: 41.0
3-Methylbutyryl-CoA: 36.3
inactive: 27.5
fatty_acid: 17.9

hbmAIII

PKS_AT (583..885): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 235.0
inactive: 125.8
Methoxymalonyl-CoA: 71.2
Methylmalonyl-CoA: 64.5
Propionyl-CoA: 38.8
Isobutyryl-CoA: 33.1
Benzoyl-CoA: 30.6
Ethylmalonyl-CoA: 21.2
Acetyl-CoA: 19.2
2-Methylbutyryl-CoA: 15.0
trans-1,2-CPDA: 14.3
CHC-CoA: 13.8
fatty_acid: 10.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (2665..2962): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.7
Ethylmalonyl-CoA: 182.6
Methoxymalonyl-CoA: 164.7
2-Methylbutyryl-CoA: 118.9
Malonyl-CoA: 105.3
Propionyl-CoA: 105.0
trans-1,2-CPDA: 95.5
CHC-CoA: 78.9
Isobutyryl-CoA: 68.6
Benzoyl-CoA: 60.1
Acetyl-CoA: 45.4
3-Methylbutyryl-CoA: 36.3
fatty_acid: 25.6
inactive: 23.6