BGC0000093 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000093 (Streptomyces nanchangensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	185,250	High	meilingmycin/Meilingmycin A/Meilingmycin B/Meilingmycin E/Meilingmycin D/Meilingmycin C	PKS:Type I

BGC0000093 - Region 1 - T1PKS

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Location: 1 - 185,250 nt. (total: 185,250 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 185,251
Label		BGC0000093

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase oxygenase

ADC45514.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ADC45536.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ADC45546.1: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

ADC45547.1: pyruvate oxidase/decarboxylase

smcogs: SMCOG1055: pyruvate oxidase/decarboxylase

ADC45553.1: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

ADC45556.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ADC45567.1: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

ADC45573.1: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

ADC45574.1: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

ADC45589.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

meiE: Heterocyclization, Hydroxylation

MITE: MITE0000010Heterocyclization, Hydroxylation (identity: 100.00%)
smcogs: SMCOG1034: cytochrome P450

meiF: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase kinase methyltransferase

ADC45521.1: methyltransferase

smcogs: SMCOG1089: methyltransferase

ADC45598.1: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

meiD: Methylation

MITE: MITE0000009Methylation (identity: 100.00%)
smcogs: SMCOG1089: methyltransferase

pks1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: amidase deacetylase hydrolase

ADC45523.1: amidase

smcogs: SMCOG1105: amidase

ADC45541.1: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

ADC45559.1: polysaccharide deacetylase

smcogs: SMCOG1235: polysaccharide deacetylase

ADC45564.1: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: decarboxylase dehydratase

ADC45566.1: L-carnitine dehydratase/bile acid-inducible

smcogs: SMCOG1189: L-carnitine dehydratase/bile acid-inducible

ADC45578.1: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Isomerases: racemase

ADC45548.1: mandelate racemase/muconate lactonizing enzyme

smcogs: SMCOG1268: mandelate racemase/muconate lactonizing enzyme

ADC45557.1: mandelate racemase/muconate lactonizing enzyme

smcogs: SMCOG1268: mandelate racemase/muconate lactonizing enzyme

Ligases: ligase synthase

ADC45517.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

meiA1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

meiA2: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

meiA3: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

meiA4: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

pks2: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000093.5	1.02	PKS	meilingmycin, Meilingmycin A, Meilingmycin B, Meilingmycin E, Meilingmycin D, Meilingmycin C	Streptomyces nanchangensis
BGC0000041.3	0.83	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.78	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001160.5	0.59	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.59	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000143.5	0.55	PKS	salinomycin	Streptomyces albus
BGC0001051.5	0.51	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0001162.3	0.51	PKS	1-heptadecene	Moorea producens JHB

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000093.5	1.00	PKS	meilingmycin, Meilingmycin A, Meilingmycin B, Meilingmycin E, Meilingmycin D, Meilingmycin C	Streptomyces nanchangensis
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 116074:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ohmal) + (Me-ccmal) + (mal - ohmal - ohmal - Me-ccmal) + (ohmal - Me-ohmal - ccmal) + (Me-redmal - ccmal - Me-ccmal)
Non-colinear ordering:: pks1 + pks2 + meiA2 + meiA4 + meiA3

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 37425:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ohmal) + (Me-ccmal)
Non-colinear ordering:: pks1 + pks2

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 19451 - 116074:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - ohmal - ohmal - Me-ccmal) + (Me-redmal - ccmal - Me-ccmal) + (ohmal - Me-ohmal - ccmal)
Non-colinear ordering:: meiA2 + meiA3 + meiA4

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 134051 - 185250:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal - redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

pks2: mmal

PKS_AT (543..834): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 188.1
Ethylmalonyl-CoA: 180.0
Methoxymalonyl-CoA: 162.9
2-Methylbutyryl-CoA: 137.0
trans-1,2-CPDA: 125.3
Propionyl-CoA: 97.1
Malonyl-CoA: 95.6
CHC-CoA: 90.1
Isobutyryl-CoA: 77.9
Benzoyl-CoA: 61.6
fatty_acid: 26.9
Acetyl-CoA: 25.7
inactive: 23.4
3-Methylbutyryl-CoA: 16.2

pks1: mal - mal

PKS_AT (366..661): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 113.4
Methylmalonyl-CoA: 37.4
inactive: 37.2
Methoxymalonyl-CoA: 34.3
Propionyl-CoA: 16.8
Ethylmalonyl-CoA: 14.7
Acetyl-CoA: 13.5
Isobutyryl-CoA: 13.3
Benzoyl-CoA: 11.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (1339..1625): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.9
inactive: 105.9
Methoxymalonyl-CoA: 67.6
Methylmalonyl-CoA: 47.2
Propionyl-CoA: 30.7
fatty_acid: 20.5
Isobutyryl-CoA: 16.6
2-Methylbutyryl-CoA: 16.5
Ethylmalonyl-CoA: 16.4
Acetyl-CoA: 16.3
CHC-CoA: 11.5
Benzoyl-CoA: 11.2
3-Methylbutyryl-CoA: 10.1
trans-1,2-CPDA: 0.0

ADC45517.1: NH2

CAL_domain (151..473): NH2

minowa: NH2

Prediction, score:

NH2: 67.4
fatty_acid: 19.9
Acetyl-CoA: 14.7
shikimic_acid: 0.0
AHBA: 0.0

meiA2: mal - mal - mal - mmal

PKS_AT (564..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 164.3
Methoxymalonyl-CoA: 66.6
inactive: 62.0
Methylmalonyl-CoA: 50.8
Ethylmalonyl-CoA: 23.8
Acetyl-CoA: 22.3
Isobutyryl-CoA: 19.1
Propionyl-CoA: 18.4
Benzoyl-CoA: 15.1
fatty_acid: 12.7
CHC-CoA: 11.6
2-Methylbutyryl-CoA: 11.5
trans-1,2-CPDA: 6.9
3-Methylbutyryl-CoA: 0.0

PKS_AT (1576..1862): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 165.2
Methoxymalonyl-CoA: 67.1
inactive: 60.8
Methylmalonyl-CoA: 51.1
Ethylmalonyl-CoA: 25.0
Acetyl-CoA: 23.2
Propionyl-CoA: 19.5
Isobutyryl-CoA: 19.4
fatty_acid: 14.7
Benzoyl-CoA: 14.6
2-Methylbutyryl-CoA: 11.9
CHC-CoA: 11.4
trans-1,2-CPDA: 9.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (3111..3397): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 62.5%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 160.4
Methoxymalonyl-CoA: 65.8
inactive: 59.4
Methylmalonyl-CoA: 49.4
Acetyl-CoA: 26.4
Ethylmalonyl-CoA: 26.2
Propionyl-CoA: 23.5
Isobutyryl-CoA: 22.0
fatty_acid: 19.9
CHC-CoA: 13.2
2-Methylbutyryl-CoA: 13.0
Benzoyl-CoA: 11.3
trans-1,2-CPDA: 8.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (4626..4924): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 194.0
Ethylmalonyl-CoA: 180.2
Methoxymalonyl-CoA: 167.5
2-Methylbutyryl-CoA: 138.9
trans-1,2-CPDA: 115.5
Propionyl-CoA: 111.5
Malonyl-CoA: 100.5
CHC-CoA: 94.6
Isobutyryl-CoA: 71.0
Benzoyl-CoA: 52.0
inactive: 38.4
Acetyl-CoA: 33.3
fatty_acid: 22.6
3-Methylbutyryl-CoA: 13.5

meiA4: mal - mmal - mal

PKS_AT (571..872): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 224.7
inactive: 112.6
Methoxymalonyl-CoA: 64.3
Methylmalonyl-CoA: 52.8
Isobutyryl-CoA: 31.6
Propionyl-CoA: 30.6
Benzoyl-CoA: 30.5
Ethylmalonyl-CoA: 24.9
Acetyl-CoA: 18.0
2-Methylbutyryl-CoA: 13.6
fatty_acid: 13.0
trans-1,2-CPDA: 10.2
CHC-CoA: 6.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (2121..2419): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.3
Ethylmalonyl-CoA: 189.3
Methoxymalonyl-CoA: 165.1
2-Methylbutyryl-CoA: 134.1
CHC-CoA: 105.9
trans-1,2-CPDA: 105.5
Malonyl-CoA: 99.1
Isobutyryl-CoA: 95.0
Propionyl-CoA: 89.9
Benzoyl-CoA: 53.5
3-Methylbutyryl-CoA: 48.7
Acetyl-CoA: 43.2
fatty_acid: 42.6
inactive: 25.0

PKS_AT (3649..3936): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.5
inactive: 89.2
Methoxymalonyl-CoA: 61.3
Methylmalonyl-CoA: 48.9
Isobutyryl-CoA: 35.8
Ethylmalonyl-CoA: 25.5
Propionyl-CoA: 23.8
Acetyl-CoA: 19.2
Benzoyl-CoA: 17.7
CHC-CoA: 13.6
2-Methylbutyryl-CoA: 13.1
fatty_acid: 12.8
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

meiA3: mmal - mal - mmal

PKS_AT (589..887): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 207.8
Ethylmalonyl-CoA: 201.4
Methoxymalonyl-CoA: 173.3
trans-1,2-CPDA: 152.4
2-Methylbutyryl-CoA: 138.1
Malonyl-CoA: 116.5
Propionyl-CoA: 111.6
CHC-CoA: 91.4
Isobutyryl-CoA: 80.1
Benzoyl-CoA: 67.5
Acetyl-CoA: 42.9
3-Methylbutyryl-CoA: 36.0
inactive: 35.8
fatty_acid: 21.7

PKS_AT (2698..2984): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 66.7%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 166.8
Methoxymalonyl-CoA: 67.3
inactive: 62.3
Methylmalonyl-CoA: 51.8
Ethylmalonyl-CoA: 24.9
Acetyl-CoA: 24.1
Isobutyryl-CoA: 20.8
Propionyl-CoA: 20.4
fatty_acid: 15.1
Benzoyl-CoA: 15.0
CHC-CoA: 11.5
2-Methylbutyryl-CoA: 11.4
trans-1,2-CPDA: 6.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (4491..4789): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 194.0
Ethylmalonyl-CoA: 180.2
Methoxymalonyl-CoA: 167.5
2-Methylbutyryl-CoA: 138.9
trans-1,2-CPDA: 115.5
Propionyl-CoA: 111.5
Malonyl-CoA: 100.5
CHC-CoA: 94.6
Isobutyryl-CoA: 71.0
Benzoyl-CoA: 52.0
inactive: 38.4
Acetyl-CoA: 33.3
fatty_acid: 22.6
3-Methylbutyryl-CoA: 13.5

meiA1: mmal - mmal - mal

PKS_AT (93..391): mmal

ATSignature: 2-Methylbutyryl-CoA

Top 3 matches:: 2-Methylbutyryl-CoA: 70.8%; Methylmalonyl-CoA: 62.5%; Isobutyryl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 155.4
Ethylmalonyl-CoA: 153.8
2-Methylbutyryl-CoA: 140.2
Methoxymalonyl-CoA: 130.8
trans-1,2-CPDA: 115.2
Propionyl-CoA: 98.5
CHC-CoA: 93.8
Malonyl-CoA: 63.7
Acetyl-CoA: 57.7
Benzoyl-CoA: 53.7
Isobutyryl-CoA: 53.0
3-Methylbutyryl-CoA: 23.1
fatty_acid: 15.8
inactive: 10.0

PKS_AT (1114..1412): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.9
Ethylmalonyl-CoA: 191.7
Methoxymalonyl-CoA: 168.9
2-Methylbutyryl-CoA: 147.7
Propionyl-CoA: 120.3
trans-1,2-CPDA: 116.4
Malonyl-CoA: 99.1
CHC-CoA: 96.4
Isobutyryl-CoA: 79.8
Benzoyl-CoA: 63.1
Acetyl-CoA: 49.9
3-Methylbutyryl-CoA: 34.4
fatty_acid: 33.9
inactive: 24.9

PKS_AT (2675..2977): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 136.3
inactive: 54.0
Methoxymalonyl-CoA: 44.9
Methylmalonyl-CoA: 33.7
Propionyl-CoA: 22.8
Acetyl-CoA: 17.2
Ethylmalonyl-CoA: 16.0
2-Methylbutyryl-CoA: 10.0
Isobutyryl-CoA: 5.2
CHC-CoA: 2.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor