BGC0000163 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000163 (Streptomyces longisporoflavus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	169,690	tetronomycin	Polyketide	54%

BGC0000163 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 169,690 nt. (total: 169,690 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 169,691
Label		BGC0000163

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000163.1	0.74	Polyketide	tetronasin	Streptomyces longisporoflavus
BGC0000041.1	0.43	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001164.1	0.34	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000122.1	0.32	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001283.1	0.32	Polyketide	arsono-polyketide	Streptomyces lividans 1326
BGC0002031.1	0.32	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0001062.1	0.32	Polyketide	TLN-05220	Micromonospora echinospora subsp. challisensis
BGC0000160.1	0.32	Polyketide	terreic acid	Aspergillus terreus NIH2624
BGC0000120.1	0.31	Polyketide	patulin	Penicillium expansum
BGC0000170.1	0.31	Polyketide	yanuthone D	Aspergillus niger ATCC 1015

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000163.1	0.93	Polyketide	tetronasin	Streptomyces longisporoflavus
BGC0000041.1	0.74	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001164.1	0.67	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000122.1	0.66	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001283.1	0.66	Polyketide	arsono-polyketide	Streptomyces lividans 1326
BGC0002031.1	0.66	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0001062.1	0.65	Polyketide	TLN-05220	Micromonospora echinospora subsp. challisensis
BGC0000160.1	0.65	Polyketide	terreic acid	Aspergillus terreus NIH2624
BGC0000120.1	0.64	Polyketide	patulin	Penicillium expansum
BGC0000170.1	0.64	Polyketide	yanuthone D	Aspergillus niger ATCC 1015

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 6100 - 156011:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - mal - ohmal) + (mal - ccmal - Me-redmal) + (Me-ccmal - ccmal - ohmal) + (Me-redmal - Me-ccmal - ccmal) + (redmal - Me-redmal - Me-ccmal) + (ohmal) + (redmal)
Non-colinear ordering:: tsnDI + tsnAI + tsnAII + tsnAIII + tsnAIV + tsnAV + tsnDIII

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

tsnAII

PKS_AT (573..871): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.3
Ethylmalonyl-CoA: 190.3
Methoxymalonyl-CoA: 161.6
2-Methylbutyryl-CoA: 135.3
CHC-CoA: 122.5
trans-1,2-CPDA: 106.6
Malonyl-CoA: 98.5
Propionyl-CoA: 96.3
Isobutyryl-CoA: 47.1
Benzoyl-CoA: 44.7
inactive: 28.9
fatty_acid: 21.5
Acetyl-CoA: 19.7
3-Methylbutyryl-CoA: 19.2

PKS_AT (2301..2595): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 145.4
inactive: 73.0
Methoxymalonyl-CoA: 46.1
Methylmalonyl-CoA: 30.7
Propionyl-CoA: 18.8
Isobutyryl-CoA: 15.0
fatty_acid: 13.7
Acetyl-CoA: 12.6
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (4050..4343): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 145.2
inactive: 74.8
Methoxymalonyl-CoA: 48.1
Methylmalonyl-CoA: 32.8
Propionyl-CoA: 22.1
Isobutyryl-CoA: 19.4
Acetyl-CoA: 14.4
fatty_acid: 13.4
trans-1,2-CPDA: 9.2
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.0

tsnAIII

PKS_AT (570..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.3
Ethylmalonyl-CoA: 190.3
Methoxymalonyl-CoA: 161.6
2-Methylbutyryl-CoA: 135.3
CHC-CoA: 122.5
trans-1,2-CPDA: 106.6
Malonyl-CoA: 98.5
Propionyl-CoA: 96.3
Isobutyryl-CoA: 47.1
Benzoyl-CoA: 44.7
inactive: 28.9
fatty_acid: 21.5
Acetyl-CoA: 19.7
3-Methylbutyryl-CoA: 19.2

PKS_AT (2630..2933): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 193.9
Ethylmalonyl-CoA: 188.7
Methoxymalonyl-CoA: 160.2
2-Methylbutyryl-CoA: 133.1
CHC-CoA: 124.6
trans-1,2-CPDA: 105.8
Propionyl-CoA: 98.9
Malonyl-CoA: 98.9
Isobutyryl-CoA: 49.4
Benzoyl-CoA: 44.5
inactive: 28.9
fatty_acid: 23.0
Acetyl-CoA: 22.0
3-Methylbutyryl-CoA: 20.9

PKS_AT (4369..4662): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 150.7
inactive: 76.2
Methoxymalonyl-CoA: 50.2
Methylmalonyl-CoA: 33.5
Propionyl-CoA: 20.4
Isobutyryl-CoA: 19.1
fatty_acid: 13.8
Acetyl-CoA: 12.6
trans-1,2-CPDA: 8.2
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.0

tsnAIV

PKS_AT (569..863): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 150.5
inactive: 77.0
Methoxymalonyl-CoA: 49.2
Methylmalonyl-CoA: 33.9
Propionyl-CoA: 19.8
Isobutyryl-CoA: 19.1
fatty_acid: 13.8
Acetyl-CoA: 12.6
trans-1,2-CPDA: 8.2
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.0

PKS_AT (2676..2979): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.3
Ethylmalonyl-CoA: 184.3
Methoxymalonyl-CoA: 156.8
2-Methylbutyryl-CoA: 126.8
CHC-CoA: 121.4
trans-1,2-CPDA: 109.3
Propionyl-CoA: 92.8
Malonyl-CoA: 92.7
Isobutyryl-CoA: 45.9
Benzoyl-CoA: 44.0
inactive: 28.7
Acetyl-CoA: 21.3
fatty_acid: 19.1
3-Methylbutyryl-CoA: 18.5

PKS_AT (4755..5058): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 192.7
Ethylmalonyl-CoA: 187.3
Methoxymalonyl-CoA: 159.9
2-Methylbutyryl-CoA: 129.5
CHC-CoA: 121.7
trans-1,2-CPDA: 105.4
Propionyl-CoA: 95.7
Malonyl-CoA: 93.3
Isobutyryl-CoA: 46.2
Benzoyl-CoA: 44.2
inactive: 28.7
fatty_acid: 20.7
Acetyl-CoA: 20.0
3-Methylbutyryl-CoA: 18.1

tsnAV

PKS_AT (563..864): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 191.8
inactive: 95.1
Methoxymalonyl-CoA: 68.6
Methylmalonyl-CoA: 47.9
Acetyl-CoA: 25.6
Isobutyryl-CoA: 24.8
Propionyl-CoA: 19.3
Ethylmalonyl-CoA: 17.3
CHC-CoA: 13.8
Benzoyl-CoA: 10.1
2-Methylbutyryl-CoA: 7.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

tsnAI

PKS_AT (581..881): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 223.0
inactive: 117.2
Methoxymalonyl-CoA: 70.9
Methylmalonyl-CoA: 55.9
Propionyl-CoA: 40.4
Isobutyryl-CoA: 31.8
Ethylmalonyl-CoA: 24.6
2-Methylbutyryl-CoA: 19.6
Benzoyl-CoA: 18.5
Acetyl-CoA: 18.4
CHC-CoA: 10.2
fatty_acid: 9.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1623..1916): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 148.5
inactive: 73.5
Methoxymalonyl-CoA: 48.3
Methylmalonyl-CoA: 35.2
Propionyl-CoA: 21.7
Isobutyryl-CoA: 19.6
fatty_acid: 14.0
Acetyl-CoA: 13.5
trans-1,2-CPDA: 8.5
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.0

PKS_AT (3401..3704): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 193.9
Ethylmalonyl-CoA: 187.6
Methoxymalonyl-CoA: 158.7
2-Methylbutyryl-CoA: 133.2
CHC-CoA: 122.4
trans-1,2-CPDA: 111.2
Malonyl-CoA: 98.4
Propionyl-CoA: 93.6
Isobutyryl-CoA: 46.9
Benzoyl-CoA: 44.6
inactive: 29.1
Acetyl-CoA: 20.8
3-Methylbutyryl-CoA: 19.6
fatty_acid: 19.5

tsnDIII

PKS_AT (534..827): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 66.7%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 142.6
inactive: 70.4
Methylmalonyl-CoA: 55.5
Methoxymalonyl-CoA: 54.1
Propionyl-CoA: 48.5
Isobutyryl-CoA: 44.1
Ethylmalonyl-CoA: 31.0
CHC-CoA: 28.5
2-Methylbutyryl-CoA: 25.5
Benzoyl-CoA: 19.1
Acetyl-CoA: 11.0
trans-1,2-CPDA: 9.7
fatty_acid: 9.0
3-Methylbutyryl-CoA: 0.0

tsn19

Search NORINE for peptide: strict or relaxed

AMP-binding (21..426): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: iGWlvgIaSK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 50% (weak)

tsnDI

PKS_AT (15..269): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 109.7
inactive: 34.9
Methylmalonyl-CoA: 30.4
Methoxymalonyl-CoA: 27.5
fatty_acid: 27.3
Isobutyryl-CoA: 17.0
trans-1,2-CPDA: 0.0
Propionyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

tsn21

CAL_domain (181..498): pk

minowa: NH2

Prediction, score:

NH2: 66.8
Acetyl-CoA: 20.3
fatty_acid: 19.4
shikimic_acid: 0.0
AHBA: 0.0