BGC0000166 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000166 (Streptomyces fradiae)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	43,280	High	tylactone	PKS

BGC0000166 - Region 1 - T1PKS

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Location: 1 - 43,280 nt. (total: 43,280 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 43,281
Label		BGC0000166

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

tylG: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

tylG_rename5: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Ligases: synthase

tylG_rename1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

tylG_rename2: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

tylG_rename3: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

tylG_rename4: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000166.5	1.00	PKS	tylactone	Streptomyces fradiae
BGC0000018.5	0.78	PKS	angolamycin	Streptomyces eurythermus
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.73	PKS	oronofacic acid	Pseudomonas syringae
BGC0002086.3	0.67	PKS	rosamicin	Micromonospora carbonacea
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001812.3	0.67	PKS	tylactone	Micromonospora chalcea subsp. izumensis
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000166.5	1.00	PKS	tylactone	Streptomyces fradiae
BGC0000018.5	0.78	PKS	angolamycin	Streptomyces eurythermus
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.73	PKS	oronofacic acid	Pseudomonas syringae
BGC0002086.3	0.67	PKS	rosamicin	Micromonospora carbonacea
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001812.3	0.67	PKS	tylactone	Micromonospora chalcea subsp. izumensis
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 43280:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ccmal - Me-ccmal) + (ccmal) + (Me-ohmal - redemal) + (Me-ohmal) + (ohmal)
Non-colinear ordering:: tylG + tylG_rename1 + tylG_rename2 + tylG_rename3 + tylG_rename4

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

tylG: mmal - mmal - mmal

PKS_AT (588..881): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 196.3
Ethylmalonyl-CoA: 174.6
Methoxymalonyl-CoA: 156.2
2-Methylbutyryl-CoA: 107.2
trans-1,2-CPDA: 96.4
Propionyl-CoA: 91.9
CHC-CoA: 91.7
Malonyl-CoA: 88.1
Isobutyryl-CoA: 70.6
3-Methylbutyryl-CoA: 48.3
Benzoyl-CoA: 41.9
Acetyl-CoA: 41.5
inactive: 26.9
fatty_acid: 25.0

PKS_AT (1608..1901): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 196.3
Ethylmalonyl-CoA: 174.6
Methoxymalonyl-CoA: 156.2
2-Methylbutyryl-CoA: 107.2
trans-1,2-CPDA: 96.4
Propionyl-CoA: 91.9
CHC-CoA: 91.7
Malonyl-CoA: 88.1
Isobutyryl-CoA: 70.6
3-Methylbutyryl-CoA: 48.3
Benzoyl-CoA: 41.9
Acetyl-CoA: 41.5
inactive: 26.9
fatty_acid: 25.0

PKS_AT (3151..3444): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 196.3
Ethylmalonyl-CoA: 174.6
Methoxymalonyl-CoA: 156.2
2-Methylbutyryl-CoA: 107.2
trans-1,2-CPDA: 96.4
Propionyl-CoA: 91.9
CHC-CoA: 91.7
Malonyl-CoA: 88.1
Isobutyryl-CoA: 70.6
3-Methylbutyryl-CoA: 48.3
Benzoyl-CoA: 41.9
Acetyl-CoA: 41.5
inactive: 26.9
fatty_acid: 25.0

tylG_rename1: mal

PKS_AT (569..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.5
inactive: 96.2
Methoxymalonyl-CoA: 90.6
Methylmalonyl-CoA: 85.4
Propionyl-CoA: 54.1
Isobutyryl-CoA: 53.6
Ethylmalonyl-CoA: 47.3
2-Methylbutyryl-CoA: 37.2
trans-1,2-CPDA: 26.9
fatty_acid: 24.5
Acetyl-CoA: 22.8
CHC-CoA: 22.7
Benzoyl-CoA: 22.2
3-Methylbutyryl-CoA: 14.3

tylG_rename2: mmal - emal

PKS_AT (590..883): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 196.3
Ethylmalonyl-CoA: 174.6
Methoxymalonyl-CoA: 156.2
2-Methylbutyryl-CoA: 107.2
trans-1,2-CPDA: 96.4
Propionyl-CoA: 91.9
CHC-CoA: 91.7
Malonyl-CoA: 88.1
Isobutyryl-CoA: 70.6
3-Methylbutyryl-CoA: 48.3
Benzoyl-CoA: 41.9
Acetyl-CoA: 41.5
inactive: 26.9
fatty_acid: 25.0

PKS_AT (2097..2395): emal

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Methylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 245.5
Methylmalonyl-CoA: 200.5
Methoxymalonyl-CoA: 179.9
trans-1,2-CPDA: 152.7
2-Methylbutyryl-CoA: 146.5
Propionyl-CoA: 120.9
CHC-CoA: 95.5
Malonyl-CoA: 84.0
Isobutyryl-CoA: 77.9
Benzoyl-CoA: 75.3
Acetyl-CoA: 60.4
fatty_acid: 41.5
3-Methylbutyryl-CoA: 40.4
inactive: 21.9

tylG_rename3: mmal

PKS_AT (584..877): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 196.3
Ethylmalonyl-CoA: 174.6
Methoxymalonyl-CoA: 156.2
2-Methylbutyryl-CoA: 107.2
trans-1,2-CPDA: 96.4
Propionyl-CoA: 91.9
CHC-CoA: 91.7
Malonyl-CoA: 88.1
Isobutyryl-CoA: 70.6
3-Methylbutyryl-CoA: 48.3
Benzoyl-CoA: 41.9
Acetyl-CoA: 41.5
inactive: 26.9
fatty_acid: 25.0

tylG_rename4: mal

PKS_AT (578..868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.0
inactive: 92.7
Methoxymalonyl-CoA: 71.2
Methylmalonyl-CoA: 64.4
Isobutyryl-CoA: 41.7
Propionyl-CoA: 35.2
Ethylmalonyl-CoA: 24.4
Acetyl-CoA: 21.3
2-Methylbutyryl-CoA: 15.0
Benzoyl-CoA: 13.2
CHC-CoA: 12.6
3-Methylbutyryl-CoA: 11.7
fatty_acid: 6.5
trans-1,2-CPDA: 0.1

Link to PARAS substrate predictor