BGC0001235 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001235 (Polyangium spumosum)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	50,952

BGC0001235 - Region 1 - NRPS,T1PKS

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Location: 1 - 50,952 nt. (total: 50,952 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 50,953
Label		BGC0001235

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001235.1	1.66	NRP, Polyketide	antalid	Polyangium spumosum
BGC0001873.1	0.50	NRP	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0001132.1	0.49	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.1	0.47	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.1	0.47	NRP	AM-toxin	Alternaria alternata
BGC0001128.1	0.47	NRP	luminmide	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.1	0.46	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.1	0.46	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001844.1	0.46	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001135.1	0.46	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001235.1	0.94	NRP, Polyketide	antalid	Polyangium spumosum
BGC0001873.1	0.59	NRP	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0001132.1	0.58	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.1	0.57	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.1	0.57	NRP	AM-toxin	Alternaria alternata
BGC0001128.1	0.56	NRP	luminmide	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.1	0.56	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.1	0.56	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001844.1	0.56	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001135.1	0.56	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Similar known gene clusters

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 50952:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal - Me-ccmal - Me-ccmal) + (gly - cys - D-X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 46663:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal - Me-ccmal - Me-ccmal) + (gly - cys - D-X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 6677 - 50952:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal - Me-ccmal - Me-ccmal) + (gly - cys - D-X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

antA

PKS_AT (672..968): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 66.7%; Methylmalonyl-CoA: 66.7%; Methoxymalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 103.7
Methoxymalonyl-CoA: 92.1
Ethylmalonyl-CoA: 77.0
Malonyl-CoA: 74.6
2-Methylbutyryl-CoA: 69.1
Propionyl-CoA: 64.3
Isobutyryl-CoA: 61.7
trans-1,2-CPDA: 60.4
CHC-CoA: 53.6
Acetyl-CoA: 50.8
fatty_acid: 36.3
Benzoyl-CoA: 35.1
3-Methylbutyryl-CoA: 28.9
inactive: 16.2

PKS_AT (1096..1396): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 205.2
Ethylmalonyl-CoA: 200.9
Methoxymalonyl-CoA: 171.8
2-Methylbutyryl-CoA: 153.8
trans-1,2-CPDA: 136.1
CHC-CoA: 109.2
Malonyl-CoA: 104.6
Propionyl-CoA: 103.8
Isobutyryl-CoA: 95.0
Benzoyl-CoA: 62.5
Acetyl-CoA: 57.6
3-Methylbutyryl-CoA: 33.5
inactive: 33.0
fatty_acid: 30.3

PKS_AT (2124..2429): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 225.6
inactive: 128.4
Methoxymalonyl-CoA: 69.2
Methylmalonyl-CoA: 54.1
Isobutyryl-CoA: 32.0
Propionyl-CoA: 24.5
Acetyl-CoA: 21.6
Benzoyl-CoA: 11.6
2-Methylbutyryl-CoA: 11.1
Ethylmalonyl-CoA: 3.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3608..3905): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 205.5
Ethylmalonyl-CoA: 199.0
Methoxymalonyl-CoA: 176.2
2-Methylbutyryl-CoA: 158.9
trans-1,2-CPDA: 129.5
Propionyl-CoA: 111.8
Malonyl-CoA: 107.8
CHC-CoA: 95.3
Isobutyryl-CoA: 89.0
Acetyl-CoA: 60.5
Benzoyl-CoA: 59.3
fatty_acid: 32.5
3-Methylbutyryl-CoA: 32.1
inactive: 28.5

PKS_AT (5384..5681): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 175.8
Ethylmalonyl-CoA: 142.3
Methoxymalonyl-CoA: 133.3
Isobutyryl-CoA: 114.9
Malonyl-CoA: 95.0
Benzoyl-CoA: 93.2
Propionyl-CoA: 84.8
trans-1,2-CPDA: 82.5
2-Methylbutyryl-CoA: 80.3
Acetyl-CoA: 71.0
CHC-CoA: 63.6
3-Methylbutyryl-CoA: 51.8
inactive: 34.6
fatty_acid: 30.2

antB

Search NORINE for peptide: strict or relaxed

AMP-binding (532..926): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DfLQVGiIfK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 70% (weak)

AMP-binding (1584..1980): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLwNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 90% (moderate)

AMP-binding (3000..3398): X

NRPSPredictor2: phe, trp, phg, tyr, bht

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAataGLImK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 60% (weak)