BGC0000833 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000833 (Streptomyces rishiriensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	PKS-like,aminocoumarin	1	40,210	High	coumermycin A1	saccharide:hybrid/tailoring+other:aminocoumarin

BGC0000833 - Region 1 - PKS-like,aminocoumarin

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Location: 1 - 40,210 nt. (total: 40,210 nt) Show pHMM detection rules used

Region on contig edge.

PKS-like: (t2pks2 or ksIII)
aminocoumarin: (novK or novJ or novI or novH or SpcDK_cou)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 40,211
Label		BGC0000833

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

couI: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

couJ: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

couK: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

couN3: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: glycosyltransferase methyltransferase transferase

couM: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

couO: methyltransferase

smcogs: SMCOG1089: methyltransferase

couV: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

Hydrolases: hydrolase

couN7: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: dehydratase

couS: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

couT: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

couH: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

couL: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

couN2: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

couN4: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000833.4	1.97	other (aminocoumarin), saccharide	coumermycin A1	Streptomyces rishiriensis
BGC0000832.4	0.99	other (aminocoumarin), saccharide	clorobiocin	Streptomyces roseochromogenus subsp. oscitans DS 12.976
BGC0001249.5	0.78	NRPS	dimethylcoprogen	Alternaria alternata
BGC0000834.5	0.78	other (aminocoumarin), saccharide	cathomycin	Streptomyces niveus
BGC0002164.2	0.65	NRPS	peramine	Epichloe festucae
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.59	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.58	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000833.4	0.99	other (aminocoumarin), saccharide	coumermycin A1	Streptomyces rishiriensis
BGC0000832.4	0.72	other (aminocoumarin), saccharide	clorobiocin	Streptomyces roseochromogenus subsp. oscitans DS 12.976
BGC0001249.5	0.71	NRPS	dimethylcoprogen	Alternaria alternata
BGC0000834.5	0.70	other (aminocoumarin), saccharide	cathomycin	Streptomyces niveus
BGC0002164.2	0.67	NRPS	peramine	Epichloe festucae
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.63	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.63	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 40210:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Tyr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 34891:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Tyr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

couH: Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (26..424): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DATVVAAVCK
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (79% 8Å match)
Stachelhaus code match:: 80% (moderate)

couL: X

AMP-binding (17..421): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TVVFVVSRIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

couN4: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (12..401): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLLYLALVCK
Nearest Stachelhaus code(s):: Pro DLLYLALVCK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor