BGC0001008 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001008 (Actinomadura madurae)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	62,812	amycolamycin A / amycolamycin B	Polyketide	41%

BGC0001008 - Region 1 - NRPS,T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 62,812 nt. (total: 62,812 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 62,813
Label		BGC0001008

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001008.1	1.49	Polyketide	maduropeptin	Actinomadura madurae
BGC0001068.1	0.83	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0000056.1	0.74	Polyketide	esperamicin	Actinomadura verrucosospora
BGC0001123.1	0.69	NRP	aspirochlorine	Aspergillus oryzae RIB40
BGC0001335.1	0.68	Alkaloid	borregomycin A, borregomycin B, borregomycin C, borregomycin D	uncultured bacterium
BGC0000889.1	0.63	Other	bagremycin A, bagremycin B	Streptomyces sp. WK-5344
BGC0001909.1	0.62	Polyketide	strobilurin	Strobilurus tenacellus
BGC0001671.1	0.62	NRP	monobactam	Agrobacterium tumefaciens
BGC0001892.1	0.61	Polyketide	botcinic acid	Botrytis cinerea B05.10
BGC0002031.1	0.61	Alkaloid	kitasetaline	Kitasatospora setae KM-6054

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001008.1	0.93	Polyketide	maduropeptin	Actinomadura madurae
BGC0000056.1	0.70	Polyketide	esperamicin	Actinomadura verrucosospora
BGC0001399.1	0.65	NRP	fellutamide B	Aspergillus nidulans FGSC A4
BGC0000826.1	0.65	Alkaloid	staurosporine	Streptomyces clavuligerus ATCC 27064
BGC0001661.1	0.64	Polyketide	mayamycin	Streptomyces sp.
BGC0002031.1	0.64	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0000813.1	0.64	Alkaloid	K-252a	Nonomuraea longicatena
BGC0001280.1	0.63	Polyketide	betaenone C, betaenone A	Phoma betae
BGC0001264.1	0.63	Polyketide	betaenone A, betaenone B, betaenone C	Phoma betae
BGC0001444.1	0.63	Other	caboxamycin	Streptomyces sp. NTK 937

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 62812:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 37890:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 17886 - 61234:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 31715 - 62812:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

mdpB

PKS_AT (548..840): mmal

ATSignature: Methylmalonyl-CoA, Malonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 79.2%; Malonyl-CoA: 79.2%; Ethylmalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 131.3
Methoxymalonyl-CoA: 115.0
Malonyl-CoA: 111.1
Ethylmalonyl-CoA: 106.3
Isobutyryl-CoA: 77.4
3-Methylbutyryl-CoA: 61.7
Propionyl-CoA: 59.9
2-Methylbutyryl-CoA: 56.2
Acetyl-CoA: 51.7
Benzoyl-CoA: 48.4
trans-1,2-CPDA: 44.2
inactive: 36.5
CHC-CoA: 35.2
fatty_acid: 19.0

mdpB2

Search NORINE for peptide: strict or relaxed

AMP-binding (33..448): dhb

NRPSPredictor2: dhb

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: dhb, sal
Small clusters prediction:: dhb, sal
Single AA prediction:: dhb

Stachelhaus prediction details:

Stachelhaus sequence:: PLPshGViCK
Nearest Stachelhaus code:: dhb
Stachelhaus code match:: 70% (weak)

mdpC1

Search NORINE for peptide: strict or relaxed

AMP-binding (504..910): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DpcQVmVIaK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 60% (weak)

mdpE

PKS_AT (618..848): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 58.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 70.9
Methoxymalonyl-CoA: 42.8
Methylmalonyl-CoA: 39.0
inactive: 31.6
Isobutyryl-CoA: 19.4
fatty_acid: 13.7
Ethylmalonyl-CoA: 10.6
2-Methylbutyryl-CoA: 9.8
Benzoyl-CoA: 9.0
trans-1,2-CPDA: 8.5
CHC-CoA: 3.5
Acetyl-CoA: 1.9
3-Methylbutyryl-CoA: 0.0
Propionyl-CoA: -1.0