BGC0000020 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000020 (Actinosynnema pretiosum subsp. auranticum)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS-like	1	82,746	ansamitocin P-3	Polyketide	80%

BGC0000020 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 82,746 nt. (total: 82,746 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 82,747
Label		BGC0000020

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000020.1	1.79	Polyketide	maytansine, ansamitocin P-3	Actinosynnema pretiosum subsp. auranticum
BGC0001511.1	1.06	Polyketide	ansamitocin P-3	Actinosynnema pretiosum subsp. pretiosum
BGC0000041.1	0.95	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001276.1	0.90	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.1	0.88	Polyketide	asperlactone	Aspergillus ochraceus
BGC0000160.1	0.82	Polyketide	terreic acid	Aspergillus terreus NIH2624
BGC0000122.1	0.79	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001504.1	0.78	Polyketide	amycomicin	Amycolatopsis sp. AA4
BGC0001335.1	0.77	Alkaloid	borregomycin A, borregomycin B, borregomycin C, borregomycin D	uncultured bacterium
BGC0001068.1	0.77	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000020.1	0.97	Polyketide	maytansine, ansamitocin P-3	Actinosynnema pretiosum subsp. auranticum
BGC0001511.1	0.77	Polyketide	ansamitocin P-3	Actinosynnema pretiosum subsp. pretiosum
BGC0000041.1	0.74	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001283.1	0.65	Polyketide	arsono-polyketide	Streptomyces lividans 1326
BGC0001504.1	0.64	Polyketide	amycomicin	Amycolatopsis sp. AA4
BGC0000120.1	0.64	Polyketide	patulin	Penicillium expansum
BGC0001273.1	0.64	Polyketide	asperlactone	Aspergillus ochraceus
BGC0000122.1	0.64	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001276.1	0.64	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001854.1	0.64	Polyketide	tricholignan A	Trichoderma harzianum

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location <1 - 82746:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal - ccmal) + (Me-ohmal) + (Me-ccmal - ccmal) + (ccmxmal - mal)
Non-colinear ordering:: asmA + asmC + asmD + asmB

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location <1 - 57041:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal - ccmal) + (ccmxmal - mal)
Non-colinear ordering:: asmA + asmB

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 37225 - 82746:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-ohmal) + (Me-ccmal - ccmal)
Non-colinear ordering:: asmC + asmD

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

asmB

PKS_AT (647..939): mxmal

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Methylmalonyl-CoA: 87.5%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 188.0
Methylmalonyl-CoA: 179.8
Ethylmalonyl-CoA: 169.5
trans-1,2-CPDA: 105.9
Malonyl-CoA: 96.2
2-Methylbutyryl-CoA: 87.4
CHC-CoA: 85.9
Propionyl-CoA: 84.2
Isobutyryl-CoA: 83.0
Acetyl-CoA: 54.1
Benzoyl-CoA: 52.3
3-Methylbutyryl-CoA: 48.7
inactive: 28.1
fatty_acid: 17.8

PKS_AT (2289..2572): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 162.1
Methoxymalonyl-CoA: 64.8
inactive: 64.0
Methylmalonyl-CoA: 54.2
Propionyl-CoA: 32.1
Ethylmalonyl-CoA: 23.9
fatty_acid: 17.2
2-Methylbutyryl-CoA: 15.1
Isobutyryl-CoA: 13.0
CHC-CoA: 11.9
Benzoyl-CoA: 11.6
trans-1,2-CPDA: 8.6
Acetyl-CoA: 5.2
3-Methylbutyryl-CoA: 0.0

asmA

CAL_domain (34..434): pk

minowa: AHBA

Prediction, score:

AHBA: 518.2
shikimic_acid: 248.9
NH2: 10.1
Acetyl-CoA: 10.0
fatty_acid: 0.0

PKS_AT (1536..1819): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 198.8
Ethylmalonyl-CoA: 183.5
Methoxymalonyl-CoA: 161.3
trans-1,2-CPDA: 121.1
2-Methylbutyryl-CoA: 113.7
Malonyl-CoA: 98.8
Propionyl-CoA: 88.3
CHC-CoA: 85.7
Isobutyryl-CoA: 74.5
Benzoyl-CoA: 62.4
inactive: 46.5
3-Methylbutyryl-CoA: 29.5
Acetyl-CoA: 27.9
fatty_acid: 24.1

PKS_AT (3572..3851): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 66.7%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 138.0
Methoxymalonyl-CoA: 51.6
inactive: 48.0
Methylmalonyl-CoA: 46.3
Ethylmalonyl-CoA: 19.5
Isobutyryl-CoA: 17.1
Benzoyl-CoA: 11.0
fatty_acid: 10.6
Propionyl-CoA: 7.2
CHC-CoA: 4.2
Acetyl-CoA: 3.8
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -1.2

asmD

PKS_AT (557..842): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 79.2%; Malonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 181.8
Ethylmalonyl-CoA: 141.0
Methoxymalonyl-CoA: 137.2
2-Methylbutyryl-CoA: 99.2
CHC-CoA: 93.0
Propionyl-CoA: 90.7
trans-1,2-CPDA: 86.0
Malonyl-CoA: 72.9
Isobutyryl-CoA: 54.6
Benzoyl-CoA: 49.7
fatty_acid: 35.8
3-Methylbutyryl-CoA: 31.0
Acetyl-CoA: 29.7
inactive: 14.4

PKS_AT (2251..2525): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 124.9
Methoxymalonyl-CoA: 60.6
Methylmalonyl-CoA: 56.3
inactive: 33.8
Propionyl-CoA: 29.9
Isobutyryl-CoA: 22.3
Ethylmalonyl-CoA: 20.3
fatty_acid: 9.8
Benzoyl-CoA: 4.8
CHC-CoA: 0.8
2-Methylbutyryl-CoA: 0.7
trans-1,2-CPDA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

asmC

PKS_AT (559..841): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.1
Ethylmalonyl-CoA: 164.9
Methoxymalonyl-CoA: 144.4
trans-1,2-CPDA: 104.9
Malonyl-CoA: 100.7
2-Methylbutyryl-CoA: 96.6
Propionyl-CoA: 81.1
CHC-CoA: 76.9
Isobutyryl-CoA: 65.6
Benzoyl-CoA: 56.4
inactive: 42.4
fatty_acid: 29.9
3-Methylbutyryl-CoA: 25.5
Acetyl-CoA: 23.7