BGC0000962 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000962 (Lyngbya majuscula)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS,NRPS-like	1	40,156	curacin A	NRP + Polyketide:Modular type I	53%

BGC0000962 - Region 1 - NRPS,NRPS-like,T1PKS

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Location: 1 - 40,156 nt. (total: 40,156 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 40,157
Label		BGC0000962

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000962.1	2.35	NRP, Polyketide	barbamide	Lyngbya majuscula
BGC0000426.1	0.77	NRP	sevadicin	Paenibacillus larvae
BGC0000465.1	0.76	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0002057.1	0.75	Polyketide	pateamine	Kiritimatiellaeota bacterium
BGC0001068.1	0.71	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0002071.1	0.70	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0000389.1	0.70	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0000056.1	0.69	Polyketide	esperamicin	Actinomadura verrucosospora
BGC0001615.1	0.68	NRP	hexose-palythine-serine, hexose-shinorine	Heteroscytonema crispum UCFS10
BGC0001629.1	0.67	NRP	Jessenipeptin	Pseudomonas sp. QS1027

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000962.1	0.92	NRP, Polyketide	barbamide	Lyngbya majuscula
BGC0000426.1	0.59	NRP	sevadicin	Paenibacillus larvae
BGC0000465.1	0.59	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0002057.1	0.59	Polyketide	pateamine	Kiritimatiellaeota bacterium
BGC0001068.1	0.57	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0002071.1	0.57	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0000389.1	0.56	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0000056.1	0.56	Polyketide	esperamicin	Actinomadura verrucosospora
BGC0001615.1	0.56	NRP	hexose-palythine-serine, hexose-shinorine	Heteroscytonema crispum UCFS10
BGC0001629.1	0.56	NRP	Jessenipeptin	Pseudomonas sp. QS1027

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 40156:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (OMe-mal) + (NMe-phe - cys)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 35433:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (OMe-mal) + (NMe-phe - cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

barD

Search NORINE for peptide: strict or relaxed

AMP-binding (10..411): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAIALGGCAK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

barE

CAL_domain (64..481): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 23.1
shikimic_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

PKS_AT (1251..1548): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.4
inactive: 185.3
Methoxymalonyl-CoA: 71.0
Methylmalonyl-CoA: 64.3
Propionyl-CoA: 39.7
Isobutyryl-CoA: 34.9
Acetyl-CoA: 33.5
Benzoyl-CoA: 30.5
2-Methylbutyryl-CoA: 26.5
Ethylmalonyl-CoA: 25.6
CHC-CoA: 16.1
3-Methylbutyryl-CoA: 15.2
fatty_acid: 9.1
trans-1,2-CPDA: 0.0

barG

Search NORINE for peptide: strict or relaxed

AMP-binding (531..923): phe

NRPSPredictor2: phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: phe, trp
Single AA prediction:: phe

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTVAAVCK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 100% (strong)

AMP-binding (2014..2412): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)