BGC0001022 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001022 (Stigmatella aurantiaca)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	49,736	phenalamide	NRP + Polyketide	70%

BGC0001022 - Region 1 - NRPS,T1PKS

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Location: 1 - 49,736 nt. (total: 49,736 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 49,737
Label		BGC0001022

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001022.1	1.22	NRP, Polyketide	myxalamid	Stigmatella aurantiaca
BGC0001164.1	0.57	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001273.1	0.51	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276.1	0.51	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.1	0.51	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001068.1	0.51	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0001275.1	0.50	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001187.1	0.49	NRP, Polyketide	xenolozoyenone	Glarea lozoyensis
BGC0001874.1	0.49	NRP, Polyketide	cyclopiazonic acid	Aspergillus flavus
BGC0001050.1	0.49	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001022.1	0.96	NRP, Polyketide	myxalamid	Stigmatella aurantiaca
BGC0001164.1	0.69	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001273.1	0.65	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276.1	0.65	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.1	0.65	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001275.1	0.64	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001162.1	0.61	Polyketide	1-heptadecene	Moorea producens JHB
BGC0001037.1	0.60	NRP, Polyketide	pseurotin, azaspirene	Aspergillus fumigatus Af293
BGC0000018.1	0.60	Polyketide	angolamycin	Streptomyces eurythermus
BGC0001627.1	0.59	NRP	isonitrile lipopeptides	Mycobacterium tuberculosis H37Rv

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 49736:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (Me-ccmal) + (pk - ohmal - ccmal) + (Me-redmal) + (ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 27822:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - ohmal - ccmal) + (Me-redmal) + (ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

mxaA

Search NORINE for peptide: strict or relaxed

AMP-binding (542..932): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 100% (strong)

mxaB2

PKS_AT (570..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 79.2%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.0
Ethylmalonyl-CoA: 155.3
Methoxymalonyl-CoA: 137.6
Malonyl-CoA: 112.7
Isobutyryl-CoA: 97.9
Benzoyl-CoA: 95.7
Acetyl-CoA: 89.4
2-Methylbutyryl-CoA: 88.2
CHC-CoA: 79.4
trans-1,2-CPDA: 78.8
Propionyl-CoA: 76.7
3-Methylbutyryl-CoA: 51.1
fatty_acid: 42.1
inactive: 26.1

mxaC

PKS_AT (560..858): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; Methylmalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 142.3
Ethylmalonyl-CoA: 134.9
Malonyl-CoA: 125.2
Methoxymalonyl-CoA: 114.4
2-Methylbutyryl-CoA: 87.3
Isobutyryl-CoA: 84.5
Acetyl-CoA: 78.6
Propionyl-CoA: 65.2
Benzoyl-CoA: 63.9
trans-1,2-CPDA: 63.1
inactive: 40.7
CHC-CoA: 35.9
3-Methylbutyryl-CoA: 34.6
fatty_acid: 29.4

PKS_AT (2373..2669): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; Methylmalonyl-CoA: 75.0%; Propionyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 138.3
Methylmalonyl-CoA: 136.2
Methoxymalonyl-CoA: 126.8
Isobutyryl-CoA: 119.6
Ethylmalonyl-CoA: 101.7
trans-1,2-CPDA: 70.2
Propionyl-CoA: 70.0
2-Methylbutyryl-CoA: 65.8
Acetyl-CoA: 65.2
Benzoyl-CoA: 64.9
3-Methylbutyryl-CoA: 58.1
inactive: 54.1
CHC-CoA: 52.3
fatty_acid: 40.1

PKS_AT (3904..4197): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.9
inactive: 163.9
Methoxymalonyl-CoA: 88.9
Methylmalonyl-CoA: 82.9
Propionyl-CoA: 70.2
Isobutyryl-CoA: 50.0
Ethylmalonyl-CoA: 47.7
2-Methylbutyryl-CoA: 45.0
CHC-CoA: 41.4
Benzoyl-CoA: 32.9
trans-1,2-CPDA: 29.4
Acetyl-CoA: 20.7
3-Methylbutyryl-CoA: 20.0
fatty_acid: 17.2

mxaD

PKS_AT (551..848): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 175.0
Ethylmalonyl-CoA: 151.3
Methoxymalonyl-CoA: 127.0
Benzoyl-CoA: 103.3
Malonyl-CoA: 92.2
trans-1,2-CPDA: 90.5
Isobutyryl-CoA: 87.1
Acetyl-CoA: 73.9
2-Methylbutyryl-CoA: 73.7
Propionyl-CoA: 63.7
CHC-CoA: 48.0
3-Methylbutyryl-CoA: 40.9
inactive: 38.1
fatty_acid: 22.8

mxaE

PKS_AT (563..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 176.7
Ethylmalonyl-CoA: 155.1
Methoxymalonyl-CoA: 140.4
Isobutyryl-CoA: 105.9
Malonyl-CoA: 95.9
Benzoyl-CoA: 92.5
2-Methylbutyryl-CoA: 76.3
trans-1,2-CPDA: 72.5
Acetyl-CoA: 64.6
Propionyl-CoA: 59.2
CHC-CoA: 52.6
3-Methylbutyryl-CoA: 50.2
inactive: 26.4
fatty_acid: 20.2

mxaF

PKS_AT (624..920): pk

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 100.0%; Ethylmalonyl-CoA: 62.5%; Methylmalonyl-CoA: 62.5%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 246.1
Benzoyl-CoA: 126.0
Methylmalonyl-CoA: 101.6
Methoxymalonyl-CoA: 87.5
Ethylmalonyl-CoA: 69.8
Malonyl-CoA: 65.9
Acetyl-CoA: 58.0
3-Methylbutyryl-CoA: 51.4
trans-1,2-CPDA: 39.3
Propionyl-CoA: 38.3
CHC-CoA: 36.6
2-Methylbutyryl-CoA: 33.5
inactive: 19.3
fatty_acid: 13.5

PKS_AT (1060..1356): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 75.0%; Malonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 189.3
Ethylmalonyl-CoA: 171.4
Methoxymalonyl-CoA: 146.2
Isobutyryl-CoA: 112.3
Malonyl-CoA: 111.5
Benzoyl-CoA: 104.9
2-Methylbutyryl-CoA: 97.4
trans-1,2-CPDA: 85.9
Propionyl-CoA: 84.0
Acetyl-CoA: 72.7
CHC-CoA: 66.1
3-Methylbutyryl-CoA: 40.9
fatty_acid: 30.1
inactive: 22.2