BGC0001597 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001597 (Actinomadura fulva subsp. indica)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	71,515	cremimycin	Polyketide	27%

BGC0001597 - Region 1 - T1PKS

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Location: 1 - 71,515 nt. (total: 71,515 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 71,516
Label		BGC0001597

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001597.1	0.78	Polyketide	fluvirucin B2	Actinomadura fulva subsp. indica
BGC0000041.1	0.41	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000236.1	0.29	Polyketide	kinamycin	Streptomyces murayamaensis
BGC0001596.1	0.28	Polyketide	fluostatins M-Q	Streptomyces albus
BGC0001504.1	0.28	Polyketide	amycomicin	Amycolatopsis sp. AA4
BGC0002019.1	0.26	Terpene	tiancilactone	Streptomyces sp. CB03234
BGC0000261.1	0.26	Polyketide	R1128	Streptomyces sp. R1128
BGC0001913.1	0.26	Polyketide	phomoidride	fungal sp. ATCC 74256
BGC0001538.1	0.26	NRP, Polyketide	caboxamycin	Streptomyces sp. NTK 937
BGC0001283.1	0.26	Polyketide	arsono-polyketide	Streptomyces lividans 1326

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001597.1	0.93	Polyketide	fluvirucin B2	Actinomadura fulva subsp. indica
BGC0000041.1	0.73	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000236.1	0.62	Polyketide	kinamycin	Streptomyces murayamaensis
BGC0001596.1	0.61	Polyketide	fluostatins M-Q	Streptomyces albus
BGC0001504.1	0.61	Polyketide	amycomicin	Amycolatopsis sp. AA4
BGC0002019.1	0.60	Terpene	tiancilactone	Streptomyces sp. CB03234
BGC0000261.1	0.60	Polyketide	R1128	Streptomyces sp. R1128
BGC0001913.1	0.59	Polyketide	phomoidride	fungal sp. ATCC 74256
BGC0001538.1	0.59	NRP, Polyketide	caboxamycin	Streptomyces sp. NTK 937
BGC0001283.1	0.59	Polyketide	arsono-polyketide	Streptomyces lividans 1326

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 2508 - 71515:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal - ohmal) + (Me-redmal - redmal) + (Me-redmal)
Non-colinear ordering:: flvP1 + flvP2 + flvP3

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

flvK

PKS_AT (20..292): pk

ATSignature: (unknown)

No matches above 50%

minowa: CHC-CoA

Prediction, score:

CHC-CoA: 13.6
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

flvM

Search NORINE for peptide: strict or relaxed

AMP-binding (16..408): X

NRPSPredictor2: val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DcffFaLItK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)

flvP1

PKS_AT (758..1055): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 83.3%; Methylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 158.8
Ethylmalonyl-CoA: 145.5
Methoxymalonyl-CoA: 133.8
2-Methylbutyryl-CoA: 91.9
Propionyl-CoA: 91.3
trans-1,2-CPDA: 86.3
Isobutyryl-CoA: 77.1
Malonyl-CoA: 76.5
CHC-CoA: 67.7
Benzoyl-CoA: 58.0
inactive: 36.6
Acetyl-CoA: 35.8
fatty_acid: 35.6
3-Methylbutyryl-CoA: 19.9

PKS_AT (2856..3149): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 168.2
inactive: 79.0
Methoxymalonyl-CoA: 64.0
Methylmalonyl-CoA: 38.9
Propionyl-CoA: 27.1
Acetyl-CoA: 23.1
Ethylmalonyl-CoA: 16.3
Isobutyryl-CoA: 15.3
trans-1,2-CPDA: 15.2
fatty_acid: 13.3
Benzoyl-CoA: 12.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

flvP2

PKS_AT (583..880): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 83.3%; Methylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 158.8
Ethylmalonyl-CoA: 145.5
Methoxymalonyl-CoA: 133.8
2-Methylbutyryl-CoA: 91.9
Propionyl-CoA: 91.3
trans-1,2-CPDA: 86.3
Isobutyryl-CoA: 77.1
Malonyl-CoA: 76.5
CHC-CoA: 67.7
Benzoyl-CoA: 58.0
inactive: 36.6
Acetyl-CoA: 35.8
fatty_acid: 35.6
3-Methylbutyryl-CoA: 19.9

PKS_AT (2688..2981): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 169.4
inactive: 80.1
Methoxymalonyl-CoA: 64.6
Methylmalonyl-CoA: 39.7
Propionyl-CoA: 26.5
Acetyl-CoA: 23.1
Ethylmalonyl-CoA: 16.0
Isobutyryl-CoA: 15.3
trans-1,2-CPDA: 15.0
fatty_acid: 12.8
Benzoyl-CoA: 12.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

flvP3

PKS_AT (651..948): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 83.3%; Methylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 158.8
Ethylmalonyl-CoA: 145.5
Methoxymalonyl-CoA: 133.8
2-Methylbutyryl-CoA: 91.9
Propionyl-CoA: 91.3
trans-1,2-CPDA: 86.3
Isobutyryl-CoA: 77.1
Malonyl-CoA: 76.5
CHC-CoA: 67.7
Benzoyl-CoA: 58.0
inactive: 36.6
Acetyl-CoA: 35.8
fatty_acid: 35.6
3-Methylbutyryl-CoA: 19.9

flvN

Search NORINE for peptide: strict or relaxed

AMP-binding (39..433): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DyypTtmrVK
Nearest Stachelhaus code:: tyr
Stachelhaus code match:: 40% (weak)