BGC0000212 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000212 (Streptomyces sp. SPB074)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS,PKS-like	1	72,510	High	cinerubin B	PKS:Type II

BGC0000212 - Region 1 - PKS-like,T2PKS

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Location: 1 - 72,510 nt. (total: 72,510 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)
PKS-like: (t2pks2 or ksIII)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 72,511
Label		BGC0000212

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase oxygenase

SSBG_00485: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

SSBG_00496: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

SSBG_00508: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

SSBG_00509: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

SSBG_00512: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: acylase aminotransferase glycosyltransferase methyltransferase transferase

SSBG_00484: methyltransferase

smcogs: SMCOG1248: methyltransferase

SSBG_00489: methyltransferase

smcogs: SMCOG1089: methyltransferase

SSBG_00490: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

SSBG_00498: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

SSBG_00502: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

SSBG_00503: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

SSBG_00504: methyltransferase

smcogs: SMCOG1248: methyltransferase

SSBG_00510: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

SSBG_00513: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

Lyases: cyclase dehydratase

SSBG_00497: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

SSBG_00500: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

SSBG_00511: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

SSBG_00491: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

SSBG_00493: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

SSBG_00494: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000212.5	1.87	PKS	cinerubin B	Streptomyces sp. SPB074
BGC0000191.5	1.27	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000192.3	1.23	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000193.3	0.88	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000217.5	0.77	PKS	daunorubicin	Streptomyces sp.
BGC0000231.5	0.69	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.66	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000218.5	0.65	PKS	doxorubicin	Streptomyces peucetius
BGC0002276.2	0.59	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002154.2	0.59	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000212.5	1.00	PKS	cinerubin B	Streptomyces sp. SPB074
BGC0000191.5	0.86	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000192.3	0.85	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000193.3	0.82	PKS	aclacinomycin	Streptomyces galilaeus
BGC0000217.5	0.71	PKS	daunorubicin	Streptomyces sp.
BGC0000231.5	0.68	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.66	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0000218.5	0.66	PKS	doxorubicin	Streptomyces peucetius
BGC0002276.2	0.63	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

SSBG_00490: mal

PKS_AT (18..302): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 39.4
Methylmalonyl-CoA: 23.7
Methoxymalonyl-CoA: 23.4
Propionyl-CoA: 17.3
inactive: 11.6
Isobutyryl-CoA: 7.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 72510
Starter unit(s):: propionyl-CoA (Score: 992.0; E-value: 0)
Malonyl elongations:: 8|9 (Score: 674.1; E-value: 1.4e-205)
Product class(es):: anthracycline
Product molecular weight(s):: propionyl-CoA_8: 707 Da
propionyl-CoA_9: 749 Da

SSBG_00484: MET carboxy_O (Score: 225.6; E-value: 1.3e-69)
SSBG_00488: CYC C2-C19 (Score: 274.6; E-value: 3.2e-85)
SSBG_00489: MET carboxy_O (Score: 420.5; E-value: 5.1e-129)
SSBG_00490: AT (Score: 455.3; E-value: 2.9e-139); AT AAF70110.1_Aclacinomycin_propionyl-CoA (Score: 478.0; E-value: 1.08e-173)
SSBG_00491: KSIII (Score: 494.9; E-value: 2.7e-151); KSIII BAB72048.1_Aklavinone_propionyl-CoA (Score: 514.0; E-value: 0.0)
SSBG_00492: ACP (Score: 109.5; E-value: 1.4e-34)
SSBG_00493: CLF 8|9 (Score: 674.1; E-value: 1.4e-205)
SSBG_00494: KS (Score: 731.9; E-value: 4.7e-223)
SSBG_00496: KR C9 (Score: 480.1; E-value: 3.6e-147)
SSBG_00497: CYC C7-C12 (Score: 508.2; E-value: 1.5e-155)
SSBG_00498: GT (Score: 510.7; E-value: 8.1e-156)
SSBG_00505: CYC C5-C14/C3-C16 (Score: 441.2; E-value: 2.6e-135)
SSBG_00507: CYC C1-C18|C2-C19 (Score: 189.0; E-value: 8e-59)
SSBG_00508: KR C17 (Score: 467.7; E-value: 2e-143)
SSBG_00510: GT (Score: 458.6; E-value: 5.4e-140)