BGC0000608 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000608 (Actinokineospora fastidiosa)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	thiopeptide	1	4,495	nocathiacin	RiPP:Thiopeptide	9%

BGC0000608 - Region 1 - thiopeptide

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Location: 1 - 4,495 nt. (total: 4,495 nt) Show pHMM detection rules used

Region on contig edge.

thiopeptide: ((YcaO or TIGR03604) and ((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or PF07366 or PF06968 or PF04055) or thiostrepton)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

MIBiG comparison

KnownClusterBlast

Thiopeptides

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 4,496
Label		BGC0000608

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000608.1	1.00	RiPP	nocathiacin	Actinokineospora fastidiosa
BGC0001707.1	0.25	RiPP	nosiheptide	Streptomyces sp.
BGC0000610.1	0.21	RiPP	nosiheptide	Streptomyces actuosus
BGC0000609.1	0.20	RiPP	nocathiacin	Nocardia sp. ATCC 202099
BGC0001607.1	0.13	Other	gilvusmycin	Streptomyces sp. NRRL B-1347
BGC0000604.1	0.11	RiPP	GE2270A	Nonomuraea sp. WU8817
BGC0000613.1	0.11	RiPP	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0001155.1	0.11	RiPP	GE2270	Planobispora rosea
BGC0001860.1	0.11	RiPP	thiomuracin	Thermobispora bispora DSM 43833
BGC0001020.1	0.04	NRP, Polyketide	muraymycin C1	Streptomyces sp. NRRL 30471

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000608.1	1.00	RiPP	nocathiacin	Actinokineospora fastidiosa
BGC0001707.1	0.58	RiPP	nosiheptide	Streptomyces sp.
BGC0000610.1	0.54	RiPP	nosiheptide	Streptomyces actuosus
BGC0000609.1	0.52	RiPP	nocathiacin	Nocardia sp. ATCC 202099
BGC0001607.1	0.42	Other	gilvusmycin	Streptomyces sp. NRRL B-1347
BGC0000604.1	0.38	RiPP	GE2270A	Nonomuraea sp. WU8817
BGC0000613.1	0.37	RiPP	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0001155.1	0.37	RiPP	GE2270	Planobispora rosea
BGC0001860.1	0.37	RiPP	thiomuracin	Thermobispora bispora DSM 43833
BGC0001020.1	0.18	NRP, Polyketide	muraymycin C1	Streptomyces sp. NRRL 30471

Similar known gene clusters

Thiopeptide predictions

Thiopeptide(s)

ADH93699.1

Leader:

MSADLSALNIDSLEISEFLDDSRLEDSEVVAKVMSA

Core:

SCTTCECSCSCSS

Core with dehydrations:

DhaCysDhbDhbCysECysDhaCysDhaCysDhaDha

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Types:

Type I: Featuring genes encoding indolic acid side ring (series e)
Type II: Featuring the gene coding for quinaldic acid moiety formation (series a, b, and c)
Type III: No genes for synthesizing L-Trp derivatives (series d)

Thiopeptides

Core peptide predictions

ADH93699.1

Putative Type I

Cleavage pHMM score: 11.30

RODEO score: 24

Monoisotopic mass: 1299.4 Da

Molecular weight: 1300.4 Da

Predicted peptide features:
Central ring: pyridine tetrasubstituted (hydroxyl group present); second macrocycle

Macrocycle: 26-member

Considering maturation reactions

Monoisotopic mass: 1277.2 Da

Molecular weight: 1278.3 Da

Alternative weights: (assuming N unmodified Ser/Thr residues); 1318.5 Da N = 1; 1336.5 Da N = 2; 1354.5 Da N = 3; 1372.5 Da N = 4; 1390.5 Da N = 5; 1408.6 Da N = 6; 1426.6 Da N = 7