BGC0000981 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000981 (Aphanizomenon sp. 10E6)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS-like	1	57,112	cylindrospermopsin	NRP + Alkaloid + Polyketide:Modular type I	85%

BGC0000981 - Region 1 - NRPS-like,T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 57,112 nt. (total: 57,112 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 57,113
Label		BGC0000981

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000981.1	1.35	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E6
BGC0000979.1	1.27	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980.1	1.27	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0000978.1	0.53	NRP, Polyketide, Alkaloid	cylindrospermopsin	Cylindrospermopsis raciborskii AWT205
BGC0000041.1	0.51	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000056.1	0.46	Polyketide	esperamicin	Actinomadura verrucosospora
BGC0000046.1	0.45	Polyketide	depudecin	Alternaria brassicicola
BGC0002057.1	0.45	Polyketide	pateamine	Kiritimatiellaeota bacterium
BGC0001164.1	0.45	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.1	0.44	Polyketide	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000981.1	0.90	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E6
BGC0000979.1	0.88	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980.1	0.88	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0000978.1	0.61	NRP, Polyketide, Alkaloid	cylindrospermopsin	Cylindrospermopsis raciborskii AWT205
BGC0000046.1	0.60	Polyketide	depudecin	Alternaria brassicicola
BGC0000041.1	0.59	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001794.1	0.58	Polyketide	swainsonine	Alternaria oxytropis
BGC0001068.1	0.58	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0000056.1	0.56	Polyketide	esperamicin	Actinomadura verrucosospora
BGC0002057.1	0.56	Polyketide	pateamine	Kiritimatiellaeota bacterium

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 57112:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (X - Me-ccmal) + (ohmal) + (ccmal) + (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

cyrF

PKS_AT (553..855): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 100.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 190.5
inactive: 178.5
Methoxymalonyl-CoA: 56.4
Methylmalonyl-CoA: 42.4
Isobutyryl-CoA: 33.4
Propionyl-CoA: 31.6
Acetyl-CoA: 19.3
Benzoyl-CoA: 15.2
3-Methylbutyryl-CoA: 12.5
2-Methylbutyryl-CoA: 12.2
trans-1,2-CPDA: 11.9
Ethylmalonyl-CoA: 9.6
fatty_acid: 0.0
CHC-CoA: 0.0

cyrD

PKS_AT (565..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 197.9
inactive: 186.7
Methoxymalonyl-CoA: 54.7
Methylmalonyl-CoA: 50.1
Propionyl-CoA: 42.2
Isobutyryl-CoA: 29.3
2-Methylbutyryl-CoA: 17.5
Acetyl-CoA: 13.9
Benzoyl-CoA: 13.3
Ethylmalonyl-CoA: 9.0
3-Methylbutyryl-CoA: 7.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0

cyrC

PKS_AT (568..879): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 205.3
inactive: 169.4
Methoxymalonyl-CoA: 67.6
Methylmalonyl-CoA: 67.5
Propionyl-CoA: 42.8
Isobutyryl-CoA: 41.2
Benzoyl-CoA: 31.4
trans-1,2-CPDA: 24.4
Acetyl-CoA: 22.7
2-Methylbutyryl-CoA: 21.2
Ethylmalonyl-CoA: 21.1
CHC-CoA: 19.5
fatty_acid: 10.0
3-Methylbutyryl-CoA: 8.7

cyrB

Search NORINE for peptide: strict or relaxed

AMP-binding (28..469): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DfhFItHdAK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 60% (weak)

PKS_AT (1210..1512): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 95.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.5
inactive: 184.5
Methoxymalonyl-CoA: 74.8
Methylmalonyl-CoA: 62.4
Propionyl-CoA: 40.9
Isobutyryl-CoA: 28.9
Benzoyl-CoA: 26.2
Ethylmalonyl-CoA: 25.8
Acetyl-CoA: 19.4
2-Methylbutyryl-CoA: 18.8
CHC-CoA: 16.3
trans-1,2-CPDA: 13.8
fatty_acid: 11.3
3-Methylbutyryl-CoA: 8.3

cyrE

PKS_AT (548..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.9
inactive: 182.6
Methoxymalonyl-CoA: 70.8
Methylmalonyl-CoA: 62.0
Propionyl-CoA: 55.1
Acetyl-CoA: 41.3
Isobutyryl-CoA: 39.0
2-Methylbutyryl-CoA: 28.6
Benzoyl-CoA: 25.3
Ethylmalonyl-CoA: 22.6
trans-1,2-CPDA: 19.1
CHC-CoA: 13.8
fatty_acid: 13.1
3-Methylbutyryl-CoA: 10.6