BGC0001024 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001024 (Stigmatella aurantiaca DW4/3-1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	42,603	fulvuthiacene A / fulvuthiacene B	NRP + Polyketide	20%

BGC0001024 - Region 1 - NRPS,T1PKS

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Location: 1 - 42,603 nt. (total: 42,603 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 42,604
Label		BGC0001024

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001024.1	1.61	NRP, Polyketide	myxothiazol	Stigmatella aurantiaca DW4/3-1
BGC0001050.1	0.64	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0000041.1	0.63	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001010.1	0.61	NRP, Polyketide	melithiazol A	Melittangium lichenicola
BGC0001009.1	0.61	NRP, Polyketide	melithiazol A	Melittangium lichenicola
BGC0000982.1	0.57	NRP, Polyketide	cystothiazole A	Cystobacter fuscus
BGC0001941.1	0.56	NRP, Polyketide	fulvuthiacene A, fulvuthiacene B	Myxococcus fulvus
BGC0001023.1	0.54	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001876.1	0.52	NRP	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0000416.1	0.52	NRP	rhabdopeptide 1, rhabdopeptide 2, rhabdopeptide 3, rhabdopeptide 4	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001024.1	0.93	NRP, Polyketide	myxothiazol	Stigmatella aurantiaca DW4/3-1
BGC0001050.1	0.65	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0000041.1	0.65	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001010.1	0.64	NRP, Polyketide	melithiazol A	Melittangium lichenicola
BGC0001009.1	0.64	NRP, Polyketide	melithiazol A	Melittangium lichenicola
BGC0000982.1	0.62	NRP, Polyketide	cystothiazole A	Cystobacter fuscus
BGC0001941.1	0.61	NRP, Polyketide	fulvuthiacene A, fulvuthiacene B	Myxococcus fulvus
BGC0001023.1	0.60	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001876.1	0.60	NRP	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0000416.1	0.59	NRP	rhabdopeptide 1, rhabdopeptide 2, rhabdopeptide 3, rhabdopeptide 4	Xenorhabdus nematophila ATCC 19061

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 42603:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (cys) + (cys - ccmal) + (OMe-Me-ohmal) + (OMe-mal) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

mtaB

PKS_AT (558..855): pk

ATSignature: 3-Methylbutyryl-CoA

Top 3 matches:: 3-Methylbutyryl-CoA: 100.0%

minowa: 3-Methylbutyryl-CoA

Prediction, score:

3-Methylbutyryl-CoA: 272.9
Isobutyryl-CoA: 79.5
Methylmalonyl-CoA: 78.6
Methoxymalonyl-CoA: 73.1
Benzoyl-CoA: 58.0
2-Methylbutyryl-CoA: 57.6
Propionyl-CoA: 48.6
Acetyl-CoA: 34.3
trans-1,2-CPDA: 33.5
Malonyl-CoA: 22.2
CHC-CoA: 18.2
inactive: 18.1
fatty_acid: 14.1
Ethylmalonyl-CoA: -2.7

PKS_AT (986..1281): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 124.9
Methylmalonyl-CoA: 108.4
Methoxymalonyl-CoA: 92.3
Ethylmalonyl-CoA: 70.8
Isobutyryl-CoA: 55.9
Propionyl-CoA: 54.2
trans-1,2-CPDA: 49.3
Benzoyl-CoA: 42.5
fatty_acid: 39.5
inactive: 37.1
3-Methylbutyryl-CoA: 32.5
2-Methylbutyryl-CoA: 29.8
CHC-CoA: 24.7
Acetyl-CoA: 23.1

PKS_AT (2758..3053): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Isobutyryl-CoA: 79.2%; Malonyl-CoA: 66.7%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 153.5
Methylmalonyl-CoA: 119.6
Malonyl-CoA: 104.5
Methoxymalonyl-CoA: 95.7
Ethylmalonyl-CoA: 61.4
Benzoyl-CoA: 60.6
inactive: 42.8
Propionyl-CoA: 31.4
Acetyl-CoA: 28.3
3-Methylbutyryl-CoA: 25.6
2-Methylbutyryl-CoA: 25.5
trans-1,2-CPDA: 25.4
fatty_acid: 18.8
CHC-CoA: 16.9

mtaC

Search NORINE for peptide: strict or relaxed

AMP-binding (482..879): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNmSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 90% (moderate)

mtaD

Search NORINE for peptide: strict or relaxed

AMP-binding (538..937): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

PKS_AT (1987..2281): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 172.0
inactive: 134.0
Methoxymalonyl-CoA: 72.8
Methylmalonyl-CoA: 65.3
Propionyl-CoA: 38.9
Isobutyryl-CoA: 29.4
2-Methylbutyryl-CoA: 25.5
Ethylmalonyl-CoA: 24.5
CHC-CoA: 22.4
Benzoyl-CoA: 21.9
Acetyl-CoA: 18.3
trans-1,2-CPDA: 14.0
fatty_acid: 10.0
3-Methylbutyryl-CoA: 6.4

mtaE

PKS_AT (576..873): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 79.2%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 178.4
Ethylmalonyl-CoA: 146.8
Methoxymalonyl-CoA: 139.4
Isobutyryl-CoA: 128.0
Benzoyl-CoA: 111.0
Malonyl-CoA: 89.0
Acetyl-CoA: 88.5
2-Methylbutyryl-CoA: 84.5
trans-1,2-CPDA: 76.1
Propionyl-CoA: 70.1
CHC-CoA: 66.0
3-Methylbutyryl-CoA: 45.7
fatty_acid: 39.0
inactive: 28.0

mtaF

PKS_AT (566..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 172.0
inactive: 134.0
Methoxymalonyl-CoA: 72.8
Methylmalonyl-CoA: 65.3
Propionyl-CoA: 38.9
Isobutyryl-CoA: 29.4
2-Methylbutyryl-CoA: 25.5
Ethylmalonyl-CoA: 24.5
CHC-CoA: 22.4
Benzoyl-CoA: 21.9
Acetyl-CoA: 18.3
trans-1,2-CPDA: 14.0
fatty_acid: 10.0
3-Methylbutyryl-CoA: 6.4

mtaG

Search NORINE for peptide: strict or relaxed

A-OX (547..1308): X