BGC0001212 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001212 (Nannocystis sp. MB1016)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	55,222	chondramide A	NRP:Cyclic depsipeptide + Polyketide:Modular type I	30%

BGC0001212 - Region 1 - NRPS,T1PKS

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Location: 1 - 55,222 nt. (total: 55,222 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 55,223
Label		BGC0001212

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001212.1	1.32	NRP, Polyketide	nannocystin a	Nannocystis sp. MB1016
BGC0000426.1	0.75	NRP	sevadicin	Paenibacillus larvae
BGC0001615.1	0.67	NRP	hexose-palythine-serine, hexose-shinorine	Heteroscytonema crispum UCFS10
BGC0000389.1	0.66	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0002071.1	0.66	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0002075.1	0.64	NRP, Alkaloid	Pyreudione A, Pyreudione B, Pyreudione C, Pyreudione D, Pyreudione E	Pseudomonas fluorescens
BGC0001023.1	0.60	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001068.1	0.58	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0001858.1	0.57	Polyketide	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0000041.1	0.56	Polyketide	oronofacic acid	Pseudomonas syringae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001212.1	0.93	NRP, Polyketide	nannocystin a	Nannocystis sp. MB1016
BGC0000426.1	0.70	NRP	sevadicin	Paenibacillus larvae
BGC0001615.1	0.67	NRP	hexose-palythine-serine, hexose-shinorine	Heteroscytonema crispum UCFS10
BGC0000389.1	0.66	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0002071.1	0.66	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0002075.1	0.65	NRP, Alkaloid	Pyreudione A, Pyreudione B, Pyreudione C, Pyreudione D, Pyreudione E	Pseudomonas fluorescens
BGC0001023.1	0.64	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001275.1	0.63	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.1	0.63	Polyketide	asperlactone	Aspergillus ochraceus
BGC0000067.1	0.63	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 55222:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - Me-ccmal) + (OMe-ohmal) + (Me-ccmal) + (NMe-X) + (D-X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ncyA

PKS_AT (529..824): pk

ATSignature: Benzoyl-CoA

Top 3 matches:: Benzoyl-CoA: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Benzoyl-CoA

Prediction, score:

Benzoyl-CoA: 157.3
Isobutyryl-CoA: 120.6
Methylmalonyl-CoA: 114.9
Methoxymalonyl-CoA: 97.0
Ethylmalonyl-CoA: 88.9
Acetyl-CoA: 71.4
3-Methylbutyryl-CoA: 69.5
Propionyl-CoA: 65.0
Malonyl-CoA: 59.9
2-Methylbutyryl-CoA: 57.3
CHC-CoA: 56.2
trans-1,2-CPDA: 51.8
fatty_acid: 35.4
inactive: 22.8

PKS_AT (947..1243): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 70.8%; Methoxymalonyl-CoA: 66.7%; Malonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 117.9
Methoxymalonyl-CoA: 107.3
Ethylmalonyl-CoA: 86.7
Isobutyryl-CoA: 81.7
Malonyl-CoA: 66.7
Propionyl-CoA: 64.0
2-Methylbutyryl-CoA: 56.3
Benzoyl-CoA: 46.1
trans-1,2-CPDA: 44.8
Acetyl-CoA: 43.1
CHC-CoA: 36.4
fatty_acid: 26.9
3-Methylbutyryl-CoA: 23.9
inactive: 21.3

ncyB

PKS_AT (702..999): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 190.1
inactive: 105.8
Methoxymalonyl-CoA: 75.7
Methylmalonyl-CoA: 72.3
Propionyl-CoA: 47.9
Ethylmalonyl-CoA: 46.8
Isobutyryl-CoA: 40.4
Benzoyl-CoA: 35.6
Acetyl-CoA: 33.0
CHC-CoA: 27.0
2-Methylbutyryl-CoA: 26.5
trans-1,2-CPDA: 21.1
fatty_acid: 14.9
3-Methylbutyryl-CoA: 12.2

PKS_AT (2598..2894): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 182.6
Ethylmalonyl-CoA: 149.2
Methoxymalonyl-CoA: 139.7
2-Methylbutyryl-CoA: 113.4
Malonyl-CoA: 112.6
Isobutyryl-CoA: 102.5
Propionyl-CoA: 95.6
trans-1,2-CPDA: 93.0
Benzoyl-CoA: 88.5
CHC-CoA: 71.6
Acetyl-CoA: 60.2
3-Methylbutyryl-CoA: 45.0
inactive: 38.0
fatty_acid: 36.7

ncyC

PKS_AT (616..911): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 79.2%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 191.8
inactive: 128.7
Methoxymalonyl-CoA: 67.4
Methylmalonyl-CoA: 51.0
Propionyl-CoA: 48.2
Isobutyryl-CoA: 36.8
Acetyl-CoA: 30.4
2-Methylbutyryl-CoA: 24.8
CHC-CoA: 24.4
Ethylmalonyl-CoA: 18.3
Benzoyl-CoA: 17.4
3-Methylbutyryl-CoA: 15.0
fatty_acid: 13.5
trans-1,2-CPDA: 10.4

ncyD

PKS_AT (626..921): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 168.8
Ethylmalonyl-CoA: 153.6
Methoxymalonyl-CoA: 134.9
Malonyl-CoA: 105.5
Isobutyryl-CoA: 103.7
Benzoyl-CoA: 92.4
2-Methylbutyryl-CoA: 91.4
Propionyl-CoA: 82.1
trans-1,2-CPDA: 76.4
CHC-CoA: 68.8
inactive: 53.2
Acetyl-CoA: 52.5
3-Methylbutyryl-CoA: 47.7
fatty_acid: 31.0

ncyE

Search NORINE for peptide: strict or relaxed

AMP-binding (530..928): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGaFLGiilK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 60% (weak)

ncyF

Search NORINE for peptide: strict or relaxed

AMP-binding (536..929): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DgacVAlIlK
Nearest Stachelhaus code:: bht
Stachelhaus code match:: 50% (weak)

AMP-binding (2050..2440): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DylSnAitYK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 50% (weak)