BGC0002081 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002081 (Streptomyces sp. Tue6314)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS,PKS-like	1	30,441	High	streptoketide A/streptoketide B/streptoketide C/S2502/S2507/UWM5	PKS

BGC0002081 - Region 1 - PKS-like,T2PKS

Download region GenBank file

Download region SVG

Location: 1 - 30,441 nt. (total: 30,441 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)
PKS-like: (t2pks2 or ksIII)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 30,442
Label		BGC0002081

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

QED90618.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

QED90621.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

QED90622.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

QED90623.1: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

QED90625.1: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: acylase transferase

QED90627.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

QED90628.1: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Lyases: cyclase

QED90619.1: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

Ligases: ligase synthase

QED90612.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

QED90620.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

QED90626.1: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

QED90637.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002081.3	1.90	PKS	streptoketide A, streptoketide B, streptoketide C, S2502, S2507, UWM5	Streptomyces sp. Tue6314
BGC0000231.5	0.86	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.62	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002154.2	0.59	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.59	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001273.3	0.58	PKS	asperlactone	Aspergillus ochraceus
BGC0001723.5	0.56	PKS	oviedomycin	Streptomyces antibioticus
BGC0000272.3	0.55	PKS	spore pigment	Streptomyces collinus
BGC0001160.5	0.55	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002276.2	0.55	NRPS	choline	Aspergillus nidulans FGSC A4

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002081.3	0.98	PKS	streptoketide A, streptoketide B, streptoketide C, S2502, S2507, UWM5	Streptomyces sp. Tue6314
BGC0000231.5	0.74	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.64	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.63	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0001723.5	0.61	PKS	oviedomycin	Streptomyces antibioticus
BGC0000272.3	0.61	PKS	spore pigment	Streptomyces collinus
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002276.2	0.61	NRPS	choline	Aspergillus nidulans FGSC A4

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

QED90620.1: X

AMP-binding (16..415): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: PMNYAGGPLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

QED90627.1: mal

PKS_AT (18..296): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 52.1
Methylmalonyl-CoA: 32.2
Propionyl-CoA: 20.0
inactive: 19.9
Methoxymalonyl-CoA: 16.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 30441
Starter unit(s):: propionyl-CoA (Score: 716.0; E-value: 3.7e-133)
malonamyl-CoA (Score: 478.0; E-value: 1.38e-165)
Malonyl elongations:: 8|9 (Score: 575.8; E-value: 9.1e-176)
Product class(es):: anthracycline
aureolic acid
tetracycline
Product molecular weight(s):: malonamyl-CoA_8: 382 Da
malonamyl-CoA_9: 424 Da
propionyl-CoA_8: 353 Da
propionyl-CoA_9: 395 Da

QED90611.1: ACP (Score: 120.5; E-value: 5.2e-38)
QED90612.1: CLF 8|9 (Score: 575.8; E-value: 9.1e-176)
QED90614.1: CYC C1-C18|C2-C19 (Score: 188.2; E-value: 1.4e-58)
QED90618.1: KR C9 (Score: 448.7; E-value: 1.3e-137)
QED90619.1: CYC C7-C12 (Score: 420.2; E-value: 8.8e-129)
QED90620.1: LIG (Score: 537.5; E-value: 6.1e-164); LIG ELQ83297.1_Oxytetracycline_malonamyl-CoA (Score: 478.0; E-value: 1.38e-165)
QED90621.1: KR (Score: 111.2; E-value: 1.5e-34)
QED90622.1: KR (Score: 118.9; E-value: 7.2e-37)
QED90623.1: OXY (Score: 178.2; E-value: 7.1e-55)
QED90624.1: CYC C5-C14/C3-C16 (Score: 409.9; E-value: 9.7e-126)
QED90625.1: KR (Score: 85.1; E-value: 1.5e-26)
QED90626.1: KSIII (Score: 428.1; E-value: 5.3e-131); KSIII ABC00733.1_Cosmomycin_propionyl-CoA (Score: 374.0; E-value: 3.7e-133)
QED90627.1: AT (Score: 412.7; E-value: 2.5e-126); AT BAB72049.1_Aklavinone_propionyl-CoA (Score: 342.0; E-value: 1.66e-120)
QED90637.1: KS (Score: 733.6; E-value: 1.4e-223)