BGC0002886 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002886 (Caldora penicillata FK14-30)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	37,338	Dolastatin 10	NRPS:Type I+PKS:Type I	100%

BGC0002886 - Region 1 - NRPS,T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 37,338 nt. (total: 37,338 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 37,339
Label		BGC0002886

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000465	0.73	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0002135	0.70	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002518	0.70	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0000313	0.67	NRP	beauvericin	Beauveria bassiana
BGC0000342	0.67	NRP	enniatin	Fusarium equiseti
BGC0001023	0.66	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0000343	0.65	NRP	enterobactin	Pseudomonas sp. J465
BGC0001833	0.65	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001133	0.64	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002437	0.63	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000465	0.69	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0002135	0.68	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002518	0.68	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0000313	0.67	NRP	beauvericin	Beauveria bassiana
BGC0000342	0.67	NRP	enniatin	Fusarium equiseti
BGC0001023	0.66	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0000343	0.66	NRP	enterobactin	Pseudomonas sp. J465
BGC0001833	0.66	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001133	0.65	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002437	0.65	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 37338:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NMe-Val) + (Val - NMe-Ile) + (ccmal) + (Pro) + (ohmal) + (Phe - Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

dolB

Search NORINE for peptide: strict or relaxed

AMP-binding (25..420): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWLGGTFK
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

dolC

Search NORINE for peptide: strict or relaxed

AMP-binding (568..967): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code(s):: Val DAFWIGGTFK (71% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1623..2020): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGVTFK
Nearest Stachelhaus code(s):: Ile DAFFLGVTFK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

dolF

PKS_AT (534..827): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 133.7
inactive: 88.8
Methoxymalonyl-CoA: 57.2
Methylmalonyl-CoA: 43.1
Isobutyryl-CoA: 31.6
Propionyl-CoA: 28.4
Benzoyl-CoA: 18.3
fatty_acid: 13.9
2-Methylbutyryl-CoA: 11.5
Ethylmalonyl-CoA: 11.2
CHC-CoA: 11.2
Acetyl-CoA: 7.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

dolG

Search NORINE for peptide: strict or relaxed

AMP-binding (505..908): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAQVVK
Nearest Stachelhaus code(s):: Pro DVQFIAQVVK (71% 8Å match)
Stachelhaus code match:: 100% (strong)

dolH

PKS_AT (536..832): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 107.0
inactive: 70.7
Methoxymalonyl-CoA: 42.2
Methylmalonyl-CoA: 31.2
Benzoyl-CoA: 16.3
Propionyl-CoA: 15.8
Ethylmalonyl-CoA: 14.5
2-Methylbutyryl-CoA: 12.0
CHC-CoA: 11.9
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

dolI

Search NORINE for peptide: strict or relaxed

AMP-binding (512..901): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTVAAVCK
Nearest Stachelhaus code(s):: Phe DAWTVAAVCK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1610..2010): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)