BGC0000982 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000982 (Cystobacter fuscus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	63,876	melithiazol A	NRP + Polyketide	47%

BGC0000982 - Region 1 - NRPS,T1PKS

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Location: 1 - 63,876 nt. (total: 63,876 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 63,877
Label		BGC0000982

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000982.1	1.61	NRP, Polyketide	cystothiazole A	Cystobacter fuscus
BGC0001024.1	0.82	NRP, Polyketide	myxothiazol	Stigmatella aurantiaca DW4/3-1
BGC0001050.1	0.64	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0001010.1	0.64	NRP, Polyketide	melithiazol A	Melittangium lichenicola
BGC0001009.1	0.64	NRP, Polyketide	melithiazol A	Melittangium lichenicola
BGC0000041.1	0.64	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000373.1	0.59	NRP	holomycin	Streptomyces clavuligerus ATCC 27064
BGC0001023.1	0.58	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0000347.1	0.55	NRP	equibactin	Streptococcus equi subsp. equi
BGC0002071.1	0.54	NRP	Virginiafactin	Pseudomonas sp. QS1027

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000982.1	0.93	NRP, Polyketide	cystothiazole A	Cystobacter fuscus
BGC0001024.1	0.73	NRP, Polyketide	myxothiazol	Stigmatella aurantiaca DW4/3-1
BGC0001050.1	0.65	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0001010.1	0.65	NRP, Polyketide	melithiazol A	Melittangium lichenicola
BGC0001009.1	0.65	NRP, Polyketide	melithiazol A	Melittangium lichenicola
BGC0000041.1	0.65	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000373.1	0.63	NRP	holomycin	Streptomyces clavuligerus ATCC 27064
BGC0001023.1	0.62	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0000347.1	0.61	NRP	equibactin	Streptococcus equi subsp. equi
BGC0002071.1	0.61	NRP	Virginiafactin	Pseudomonas sp. QS1027

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 63876:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (cys) + (cys - ccmal) + (OMe-Me-ohmal) + (OMe-mal) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ctaB

PKS_AT (554..848): pk

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 100.0%; Methylmalonyl-CoA: 79.2%; Malonyl-CoA: 58.3%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 233.0
Methylmalonyl-CoA: 94.0
Methoxymalonyl-CoA: 83.9
Malonyl-CoA: 83.7
Benzoyl-CoA: 40.4
Propionyl-CoA: 37.5
Ethylmalonyl-CoA: 37.4
3-Methylbutyryl-CoA: 31.7
fatty_acid: 30.6
inactive: 30.2
Acetyl-CoA: 24.6
CHC-CoA: 22.1
2-Methylbutyryl-CoA: 18.7
trans-1,2-CPDA: 7.0

ctaC

Search NORINE for peptide: strict or relaxed

AMP-binding (516..913): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSLVWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

ctaD

Search NORINE for peptide: strict or relaxed

AMP-binding (544..943): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

PKS_AT (1983..2275): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 161.8
inactive: 110.6
Methoxymalonyl-CoA: 82.6
Methylmalonyl-CoA: 66.6
Propionyl-CoA: 35.5
Isobutyryl-CoA: 29.4
2-Methylbutyryl-CoA: 24.0
Ethylmalonyl-CoA: 23.2
trans-1,2-CPDA: 20.3
CHC-CoA: 18.3
Benzoyl-CoA: 15.5
Acetyl-CoA: 9.6
fatty_acid: 9.4
3-Methylbutyryl-CoA: 7.1

ctaE

PKS_AT (571..867): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 83.3%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 176.0
Ethylmalonyl-CoA: 145.0
Methoxymalonyl-CoA: 137.7
Isobutyryl-CoA: 133.4
Benzoyl-CoA: 122.2
Malonyl-CoA: 93.8
Acetyl-CoA: 89.1
2-Methylbutyryl-CoA: 88.4
Propionyl-CoA: 75.6
trans-1,2-CPDA: 74.5
CHC-CoA: 66.0
3-Methylbutyryl-CoA: 46.0
fatty_acid: 42.7
inactive: 32.2

ctaF

PKS_AT (568..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 159.6
inactive: 109.5
Methoxymalonyl-CoA: 82.5
Methylmalonyl-CoA: 66.3
Propionyl-CoA: 35.3
Isobutyryl-CoA: 28.9
2-Methylbutyryl-CoA: 24.0
Ethylmalonyl-CoA: 23.1
trans-1,2-CPDA: 19.7
CHC-CoA: 18.3
Benzoyl-CoA: 15.6
Acetyl-CoA: 11.8
fatty_acid: 9.2
3-Methylbutyryl-CoA: 7.1

ctaG

Search NORINE for peptide: strict or relaxed

A-OX (554..1315): X