BGC0000973 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000973 (Streptomyces sp. CS40)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	46,672	collismycin A	NRPS:Type I+PKS:Type I	100%

BGC0000973 - Region 1 - NRPS,T1PKS

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Location: 1 - 46,672 nt. (total: 46,672 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 46,673
Label		BGC0000973

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000973	1.86	NRP, Polyketide	collismycin A	Streptomyces sp. CS40
BGC0000016	0.69	Other	amphotericin B	Streptomyces nodosus
BGC0001925	0.68	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0000457	0.67	NRP	vicibactin	Rhizobium etli CFN 42
BGC0000131	0.66	Polyketide	pyrrolomycin	Streptomyces sp. UC 11065
BGC0002631	0.65	RiPP	myxarylin	Pyxidicoccus fallax
BGC0002412	0.65	NRP	holomycin	Photobacterium galatheae
BGC0002091	0.64	NRP	holomycin	Yersinia ruckeri ATCC 29473
BGC0002340	0.62	NRP, Other	pyrroloformamide A, pyrroloformamide B, pyrroloformamide D, pyrroloformamide C	Streptomyces sp. CB02980
BGC0002474	0.60	NRP	agrobactin	Agrobacterium tumefaciens

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000973	1.00	NRP, Polyketide	collismycin A	Streptomyces sp. CS40
BGC0000016	0.68	Other	amphotericin B	Streptomyces nodosus
BGC0001925	0.67	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0000457	0.67	NRP	vicibactin	Rhizobium etli CFN 42
BGC0000131	0.66	Polyketide	pyrrolomycin	Streptomyces sp. UC 11065
BGC0002631	0.66	RiPP	myxarylin	Pyxidicoccus fallax
BGC0002412	0.66	NRP	holomycin	Photobacterium galatheae
BGC0002091	0.65	NRP	holomycin	Yersinia ruckeri ATCC 29473
BGC0002340	0.65	NRP, Other	pyrroloformamide A, pyrroloformamide B, pyrroloformamide D, pyrroloformamide C	Streptomyces sp. CB02980
BGC0002474	0.64	NRP	agrobactin	Agrobacterium tumefaciens

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 6547 - 46672:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - Cys) + (Leu) + (NMe-Val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

clmG2

AMP-binding (73..293): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPAVIGIIFK
Nearest Stachelhaus code(s):: Val DGWFIGIIIK (32% 8Å match); Cap DPQDIGIVNK (29% 8Å match)
Stachelhaus code match:: 60% (weak)

clmAL

Search NORINE for peptide: strict or relaxed

AMP-binding (44..459): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: VLPAQGCLSK
Nearest Stachelhaus code(s):: diOH-Bz PLPAQGVLCK (62% 8Å match); Sal PLPAQGVLNK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

clmN1

Search NORINE for peptide: strict or relaxed

PKS_AT (597..891): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 153.1
inactive: 92.9
Methoxymalonyl-CoA: 86.3
Methylmalonyl-CoA: 69.2
Propionyl-CoA: 61.2
Ethylmalonyl-CoA: 43.2
2-Methylbutyryl-CoA: 38.0
Isobutyryl-CoA: 34.0
Acetyl-CoA: 30.7
fatty_acid: 29.1
CHC-CoA: 28.2
trans-1,2-CPDA: 24.2
Benzoyl-CoA: 20.3
3-Methylbutyryl-CoA: 10.9

AMP-binding (1550..1945): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNLSLVWK
Nearest Stachelhaus code(s):: Cys DLFNLSLVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

clmN2

Search NORINE for peptide: strict or relaxed

AMP-binding (485..901): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DYWSFGLVVK
Nearest Stachelhaus code(s):: Leu DYWSFGLVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

CCC55927.1

Search NORINE for peptide: strict or relaxed

AMP-binding (59..455): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DALWLGGTFK
Nearest Stachelhaus code(s):: Val DALWLGGTFK (76% 8Å match); bOH-Val DALWLGGTFK (74% 8Å match)
Stachelhaus code match:: 100% (strong)