BGC0001017 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001017 (Microcystis aeruginosa PCC 7806)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS,NRPS-like	1	63,626	microcystin-LR / microcystin-FR / microcystin-LA / microcystin-LAba / microcystin-LM / microcystin-LV / microcystin-LL	NRP + Polyketide	100%

BGC0001017 - Region 1 - NRPS,NRPS-like,T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 63,626 nt. (total: 63,626 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 63,627
Label		BGC0001017

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001017.1	2.18	NRP, Polyketide	microcystin LR	Microcystis aeruginosa PCC 7806
BGC0000979.1	1.06	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980.1	1.06	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0000389.1	0.98	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0001132.1	0.96	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002071.1	0.95	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0000426.1	0.95	NRP	sevadicin	Paenibacillus larvae
BGC0001128.1	0.95	NRP	luminmide	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001615.1	0.93	NRP	hexose-palythine-serine, hexose-shinorine	Heteroscytonema crispum UCFS10
BGC0001833.1	0.91	NRP	icosalide A, icosalide B	Burkholderia gladioli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001017.1	0.95	NRP, Polyketide	microcystin LR	Microcystis aeruginosa PCC 7806
BGC0000389.1	0.71	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0000427.1	0.71	NRP	shinorine, 4-deoxygadusol, mycosporine glycine	Trichormus variabilis ATCC 29413
BGC0000426.1	0.71	NRP	sevadicin	Paenibacillus larvae
BGC0002071.1	0.70	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0001615.1	0.69	NRP	hexose-palythine-serine, hexose-shinorine	Heteroscytonema crispum UCFS10
BGC0000979.1	0.68	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980.1	0.68	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0001128.1	0.68	NRP	luminmide	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000375.1	0.68	NRP	indigoidine	Streptomyces chromofuscus

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 63626:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Me-ohmal) + (mal - glu) + (ccmal) + (NMe-ser - D-ala) + (leu - X) + (arg)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

mcyG

Search NORINE for peptide: strict or relaxed

AMP-binding (44..442): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: aILWVaAsGK
Nearest Stachelhaus code:: tcl
Stachelhaus code match:: 70% (weak)

PKS_AT (1214..1508): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 166.6
inactive: 121.1
Methoxymalonyl-CoA: 58.3
Methylmalonyl-CoA: 52.1
Propionyl-CoA: 31.2
Isobutyryl-CoA: 29.1
2-Methylbutyryl-CoA: 23.2
Acetyl-CoA: 22.0
Ethylmalonyl-CoA: 21.9
CHC-CoA: 19.6
Benzoyl-CoA: 18.5
trans-1,2-CPDA: 16.0
fatty_acid: 13.0
3-Methylbutyryl-CoA: 9.8

mcyE

Search NORINE for peptide: strict or relaxed

PKS_AT (561..849): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 156.8
inactive: 115.1
Methoxymalonyl-CoA: 54.6
Methylmalonyl-CoA: 44.4
Isobutyryl-CoA: 42.9
Propionyl-CoA: 36.0
Acetyl-CoA: 29.1
3-Methylbutyryl-CoA: 21.3
Benzoyl-CoA: 15.8
2-Methylbutyryl-CoA: 9.8
CHC-CoA: 8.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0

AMP-binding (2444..2839): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHSGVVGK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

mcyD

PKS_AT (2643..2927): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 180.1
inactive: 134.4
Methylmalonyl-CoA: 62.7
Methoxymalonyl-CoA: 62.1
Propionyl-CoA: 32.7
Isobutyryl-CoA: 27.7
Benzoyl-CoA: 25.4
Acetyl-CoA: 20.8
Ethylmalonyl-CoA: 20.7
trans-1,2-CPDA: 18.2
2-Methylbutyryl-CoA: 16.6
CHC-CoA: 12.5
fatty_acid: 11.8
3-Methylbutyryl-CoA: 0.0

mcyA

Search NORINE for peptide: strict or relaxed

AMP-binding (253..660): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

AMP-binding (1736..2129): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 100% (strong)

mcyB

Search NORINE for peptide: strict or relaxed

AMP-binding (477..871): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

AMP-binding (1522..1947): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DaRHvGifvK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 50% (weak)

mcyC

Search NORINE for peptide: strict or relaxed

AMP-binding (482..877): arg

NRPSPredictor2: arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: orn, lys, arg
Small clusters prediction:: arg
Single AA prediction:: arg

Stachelhaus prediction details:

Stachelhaus sequence:: DVWTIGAVDK
Nearest Stachelhaus code:: arg
Stachelhaus code match:: 100% (strong)