BGC0001053 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001053 (Archangium disciforme)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	82,868	tubulysin A / tubulysin B / tubulysin C / tubulysin D / tubulysin E / tubulysin F / tubulysin G / tubulysin H / tubulysin I	NRP + Polyketide	64%

BGC0001053 - Region 1 - NRPS,T1PKS

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Location: 1 - 82,868 nt. (total: 82,868 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 82,869
Label		BGC0001053

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001053.1	1.40	NRP, Polyketide	tubulysin A	Archangium disciforme
BGC0002071.1	0.67	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0000465.1	0.67	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000389.1	0.67	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0001344.1	0.65	NRP, Polyketide	tubulysin A, tubulysin B, tubulysin C, tubulysin D, tubulysin E, tubulysin F, tubulysin G, tubulysin H, tubulysin I	Cystobacter sp. SBCb004
BGC0001434.1	0.65	NRP	nematophin	Xenorhabdus budapestensis
BGC0001698.1	0.65	NRP	nevaltophin A, nevaltophin B, nevaltophin C, nevaltophin D	Xenorhabdus budapestensis
BGC0001023.1	0.60	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001629.1	0.60	NRP	Jessenipeptin	Pseudomonas sp. QS1027
BGC0001161.1	0.58	Polyketide	1-heptadecene	Moorea bouillonii PNG

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001053.1	0.93	NRP, Polyketide	tubulysin A	Archangium disciforme
BGC0002071.1	0.67	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0000465.1	0.67	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000389.1	0.67	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0001344.1	0.66	NRP, Polyketide	tubulysin A, tubulysin B, tubulysin C, tubulysin D, tubulysin E, tubulysin F, tubulysin G, tubulysin H, tubulysin I	Cystobacter sp. SBCb004
BGC0001434.1	0.66	NRP	nematophin	Xenorhabdus budapestensis
BGC0001698.1	0.66	NRP	nevaltophin A, nevaltophin B, nevaltophin C, nevaltophin D	Xenorhabdus budapestensis
BGC0001023.1	0.64	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001629.1	0.63	NRP	Jessenipeptin	Pseudomonas sp. QS1027
BGC0000457.1	0.63	NRP	vicibactin	Rhizobium etli CFN 42

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 15521 - 82868:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NMe-pip) + (ile - NMe-val) + (redmal - cys) + (Me-redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

tubB

Search NORINE for peptide: strict or relaxed

AMP-binding (534..939): pip

NRPSPredictor2: pip

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DIQYIAQVVK
Nearest Stachelhaus code:: pip
Stachelhaus code match:: 100% (strong)

tubC

Search NORINE for peptide: strict or relaxed

AMP-binding (540..936): ile

NRPSPredictor2: ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: ile

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGVTFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 100% (strong)

AMP-binding (1592..1993): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWlGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 90% (moderate)

tubD

Search NORINE for peptide: strict or relaxed

PKS_AT (536..825): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 162.2
Methoxymalonyl-CoA: 70.7
inactive: 69.3
Methylmalonyl-CoA: 60.1
Isobutyryl-CoA: 52.5
Propionyl-CoA: 36.1
Ethylmalonyl-CoA: 28.7
Benzoyl-CoA: 26.0
CHC-CoA: 23.9
2-Methylbutyryl-CoA: 21.9
trans-1,2-CPDA: 10.0
Acetyl-CoA: 6.8
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

AMP-binding (2643..3042): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSlIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 90% (moderate)

tubE

CAL_domain (539..973): pk

minowa: AHBA

Prediction, score:

AHBA: 18.1
fatty_acid: 4.9
NH2: 1.1
shikimic_acid: 0.0
Acetyl-CoA: 0.0

tubF

PKS_AT (536..820): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 164.5
inactive: 74.2
Methylmalonyl-CoA: 52.7
Methoxymalonyl-CoA: 52.7
Propionyl-CoA: 23.1
Ethylmalonyl-CoA: 17.5
Isobutyryl-CoA: 17.1
2-Methylbutyryl-CoA: 13.3
fatty_acid: 11.2
CHC-CoA: 10.8
Acetyl-CoA: 9.8
trans-1,2-CPDA: 9.2
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0