BGC0000972 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000972 (Escherichia coli)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	55,140	microsclerodermin M	NRP + Polyketide:Modular type I	18%

BGC0000972 - Region 1 - NRPS,T1PKS

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Location: 1 - 55,140 nt. (total: 55,140 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 55,141
Label		BGC0000972

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000972.1	1.83	NRP, Polyketide	N-myristoyl-D-asparagine, cis-7-tetradecenoyl-D-asparagine, (R)-N1-((S)-5-oxohexan-2-yl)-2-tetradecanamidosuccinamide	Escherichia coli
BGC0002058.1	0.70	Other	Reutericyclin A, Reutericyclin B, Reutericyclin C, Reutericyclin D	Streptococcus mutans B04Sm5
BGC0000457.1	0.66	NRP	vicibactin	Rhizobium etli CFN 42
BGC0001023.1	0.65	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001876.1	0.63	NRP	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0000389.1	0.61	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0001833.1	0.60	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0002071.1	0.58	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0001050.1	0.58	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0000465.1	0.58	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000972.1	1.00	NRP, Polyketide	N-myristoyl-D-asparagine, cis-7-tetradecenoyl-D-asparagine, (R)-N1-((S)-5-oxohexan-2-yl)-2-tetradecanamidosuccinamide	Escherichia coli
BGC0002058.1	0.68	Other	Reutericyclin A, Reutericyclin B, Reutericyclin C, Reutericyclin D	Streptococcus mutans B04Sm5
BGC0000457.1	0.66	NRP	vicibactin	Rhizobium etli CFN 42
BGC0001876.1	0.66	NRP	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0001023.1	0.66	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001909.1	0.65	Polyketide	strobilurin	Strobilurus tenacellus
BGC0000389.1	0.65	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0001050.1	0.65	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0001833.1	0.64	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0002071.1	0.63	NRP	Virginiafactin	Pseudomonas sp. QS1027

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 55140:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (val - redmal) + (X) + (mal) + (gly - cys) + (cys) + (asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

clbN

Search NORINE for peptide: strict or relaxed

AMP-binding (453..844): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

clbK

Search NORINE for peptide: strict or relaxed

AMP-binding (1269..1664): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

clbJ

Search NORINE for peptide: strict or relaxed

AMP-binding (507..894): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

AMP-binding (1555..1950): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

clbI

PKS_AT (531..813): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 135.2
inactive: 78.0
Methoxymalonyl-CoA: 66.0
Methylmalonyl-CoA: 56.3
Isobutyryl-CoA: 28.5
Propionyl-CoA: 26.3
Ethylmalonyl-CoA: 20.5
2-Methylbutyryl-CoA: 14.2
Benzoyl-CoA: 11.6
trans-1,2-CPDA: 11.4
CHC-CoA: 9.8
3-Methylbutyryl-CoA: 6.9
fatty_acid: 0.0
Acetyl-CoA: 0.0

clbH

Search NORINE for peptide: strict or relaxed

AMP-binding (24..422): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

AMP-binding (992..1384): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: asGsLGGiCK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 60% (weak)

clbG

PKS_AT (7..296): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 57.2
Methoxymalonyl-CoA: 41.1
Methylmalonyl-CoA: 34.3
inactive: 30.1
Ethylmalonyl-CoA: 18.1
Propionyl-CoA: 16.0
Acetyl-CoA: 14.6
Benzoyl-CoA: 12.9
2-Methylbutyryl-CoA: 12.5
CHC-CoA: 9.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

clbB

Search NORINE for peptide: strict or relaxed

AMP-binding (502..890): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DVFWtGGiFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 80% (moderate)

PKS_AT (1604..1895): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 155.9
inactive: 95.9
Methoxymalonyl-CoA: 53.4
Methylmalonyl-CoA: 38.8
Propionyl-CoA: 26.7
Isobutyryl-CoA: 21.1
fatty_acid: 18.9
CHC-CoA: 16.9
2-Methylbutyryl-CoA: 11.0
Benzoyl-CoA: 10.3
Ethylmalonyl-CoA: 9.3
Acetyl-CoA: 8.1
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0