BGC0000975 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000975 (Nostoc sp. ATCC 53789)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS,NRPS-like	1	57,306	jamaicamide A / jamaicamide B / jamaicamide C	NRP + Polyketide	15%

BGC0000975 - Region 1 - NRPS,NRPS-like,T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 57,306 nt. (total: 57,306 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 57,307
Label		BGC0000975

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000975.1	1.81	NRP, Polyketide	cryptophycin-327	Nostoc sp. ATCC 53789
BGC0000041.1	0.89	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000389.1	0.87	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0000426.1	0.86	NRP	sevadicin	Paenibacillus larvae
BGC0002071.1	0.85	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0000396.1	0.77	NRP	nodularin	Nostoc sp. 73.1
BGC0001023.1	0.76	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001128.1	0.73	NRP	luminmide	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.1	0.73	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.1	0.73	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000975.1	0.90	NRP, Polyketide	cryptophycin-327	Nostoc sp. ATCC 53789
BGC0000389.1	0.67	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0000426.1	0.66	NRP	sevadicin	Paenibacillus larvae
BGC0002071.1	0.66	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0000041.1	0.63	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001023.1	0.63	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0000396.1	0.61	NRP	nodularin	Nostoc sp. 73.1
BGC0001858.1	0.61	Polyketide	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0001068.1	0.60	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0000313.1	0.60	NRP	beauvericin	Beauveria bassiana

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 57306:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (ohmal - ccmal) + (D-NMe-bht) + (beta-ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 49223:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (ohmal - ccmal) + (D-NMe-bht) + (beta-ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

crpA

CAL_domain (60..476): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 44.8
Acetyl-CoA: 20.5
NH2: 15.7
shikimic_acid: 0.0
AHBA: -1.9

PKS_AT (1232..1530): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.4
inactive: 194.9
Methoxymalonyl-CoA: 72.6
Methylmalonyl-CoA: 60.6
Benzoyl-CoA: 34.5
Propionyl-CoA: 34.3
Isobutyryl-CoA: 33.2
Ethylmalonyl-CoA: 25.1
Acetyl-CoA: 21.5
2-Methylbutyryl-CoA: 16.7
3-Methylbutyryl-CoA: 13.3
CHC-CoA: 12.8
trans-1,2-CPDA: 10.6
fatty_acid: 0.0

crpB

PKS_AT (580..878): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 100.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 221.9
inactive: 200.9
Methoxymalonyl-CoA: 71.2
Methylmalonyl-CoA: 61.5
Isobutyryl-CoA: 39.8
Propionyl-CoA: 36.8
Benzoyl-CoA: 33.6
Ethylmalonyl-CoA: 25.2
Acetyl-CoA: 23.0
3-Methylbutyryl-CoA: 20.3
2-Methylbutyryl-CoA: 19.3
CHC-CoA: 13.5
trans-1,2-CPDA: 13.3
fatty_acid: 0.0

PKS_AT (2153..2447): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 212.3
inactive: 193.2
Methoxymalonyl-CoA: 68.7
Methylmalonyl-CoA: 58.7
Propionyl-CoA: 33.7
Benzoyl-CoA: 33.1
Isobutyryl-CoA: 32.4
Ethylmalonyl-CoA: 22.8
Acetyl-CoA: 19.3
3-Methylbutyryl-CoA: 16.2
2-Methylbutyryl-CoA: 16.0
trans-1,2-CPDA: 11.3
CHC-CoA: 11.0
fatty_acid: 0.0

crpC

Search NORINE for peptide: strict or relaxed

AMP-binding (533..923): bht

NRPSPredictor2: bht

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: tyr, bht
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAGigK
Nearest Stachelhaus code:: bht
Stachelhaus code match:: 80% (moderate)

crpD

Search NORINE for peptide: strict or relaxed

AMP-binding (479..944): beta-ala

NRPSPredictor2: beta-ala

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: gDAVFSLADK
Nearest Stachelhaus code:: beta-ala
Stachelhaus code match:: 90% (moderate)

CAL_domain (1898..2314): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 50.9
NH2: 26.2
Acetyl-CoA: 9.8
shikimic_acid: 0.0
AHBA: 0.0