BGC0001409 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001409 (Streptomyces minoensis)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T2PKS,T1PKS,PKS-like	1	59,215	polyketomycin	Polyketide:Iterative type I + Polyketide:Type II + Saccharide:Hybrid/tailoring	88%

BGC0001409 - Region 1 - PKS-like,T1PKS,T2PKS

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Location: 1 - 59,215 nt. (total: 59,215 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
PKS-like: (t2pks2 or ksIII)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 59,216
Label		BGC0001409

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001409.1	1.58	Polyketide	dutomycin	Streptomyces minoensis
BGC0000248.1	1.28	Polyketide	naphtocyclinone	Streptomyces arenae
BGC0000231.1	1.07	Polyketide	griseusin	Streptomyces griseus
BGC0000228.1	0.94	Polyketide	granaticin	Streptomyces violaceoruber
BGC0000272.1	0.83	Polyketide	spore pigment	Streptomyces collinus
BGC0000046.1	0.78	Polyketide	depudecin	Alternaria brassicicola
BGC0000217.1	0.77	Polyketide	daunorubicin	Streptomyces sp.
BGC0000218.1	0.76	Polyketide	doxorubicin	Streptomyces peucetius
BGC0000215.1	0.76	Polyketide	curamycin	Streptomyces cyaneus
BGC0002000.1	0.76	Polyketide	setomimycin	Streptomyces aurantiacus JA 4570

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001409.1	0.87	Polyketide	dutomycin	Streptomyces minoensis
BGC0000248.1	0.74	Polyketide	naphtocyclinone	Streptomyces arenae
BGC0000231.1	0.66	Polyketide	griseusin	Streptomyces griseus
BGC0001061.1	0.65	Polyketide, Saccharide	polyketomycin	Streptomyces diastatochromogenes
BGC0000228.1	0.65	Polyketide	granaticin	Streptomyces violaceoruber
BGC0000272.1	0.61	Polyketide	spore pigment	Streptomyces collinus
BGC0001983.1	0.61	Other	triacsins	Kitasatospora aureofaciens
BGC0001458.1	0.60	Other, , (, A, m, i, n, o, c, o, u, m, a, r, i, n, )	salinipostin G	Salinispora tropica CNB-440
BGC0001797.1	0.60	RiPP	telomestatin	Streptomyces anulatus
BGC0000873.1	0.60	Other	A-201A	Saccharothrix mutabilis subsp. capreolus

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Type II PKS

Predicted core structure(s)

For candidate cluster 1, location 0 - 59215:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 16245 - 59215:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 22843 - 59215:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

dutL

Search NORINE for peptide: strict or relaxed

AMP-binding (13..392): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: --mYFGGpmK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 50% (weak)

dutG

PKS_AT (552..847): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 165.7
Ethylmalonyl-CoA: 157.7
Methoxymalonyl-CoA: 138.3
trans-1,2-CPDA: 102.4
Malonyl-CoA: 94.3
2-Methylbutyryl-CoA: 86.3
Isobutyryl-CoA: 80.6
Propionyl-CoA: 76.3
Benzoyl-CoA: 69.5
CHC-CoA: 64.8
Acetyl-CoA: 61.8
3-Methylbutyryl-CoA: 44.5
inactive: 34.9
fatty_acid: 27.5

Type II PKS gene cluster prediction details

Cluster section:: 0 to 59215
Starter unit(s):: malonamyl-CoA (Score: 425.0; E-value: 4.55e-148)
propionyl-CoA (Score: 124.0; E-value: 1.6e-33)
Malonyl elongations:: 8|9 (Score: 635.0; E-value: 9.8e-194)
Product class(es):: anthracycline
aureolic acid
tetracycline
Product molecular weight(s):: malonamyl-CoA_8: 800.78 Da
malonamyl-CoA_9: 842.81 Da
propionyl-CoA_8: 771.78 Da
propionyl-CoA_9: 813.81 Da

dutA: KS (Score: 758.6; E-value: 3.6e-231)
dutB: CLF 8|9 (Score: 635.0; E-value: 9.8e-194)
dutC: ACP (Score: 109.9; E-value: 1.1e-34)
dutD: CYC C7-C12 (Score: 321.4; E-value: 1.1e-98)
dutE: CYC C1-C18|C2-C19 (Score: 228.5; E-value: 5.3e-71)
dutF: CYC C5-C14/C3-C16 (Score: 445.7; E-value: 1.1e-136)
dutG: AT (Score: 164.9; E-value: 5.7e-51); AT BAB72049.1_Aklavinone_propionyl-CoA (Score: 124.0; E-value: 1.6e-33)
dutGT1: GT (Score: 356.5; E-value: 5.5e-109)
dutGT2: GT (Score: 345.7; E-value: 1.1e-105)
dutI: KR (Score: 137.5; E-value: 1.5e-42)
dutJ: KR (Score: 196.9; E-value: 1.1e-60)
dutL: LIG (Score: 502.7; E-value: 2.2e-153); LIG ADE34493.1_SF2575_malonamyl-CoA (Score: 425.0; E-value: 4.55e-148)
dutMT1: MET C6|C8 (Score: 546.9; E-value: 3.4e-167)
dutMT2: MET C11O (Score: 472.9; E-value: 1.2e-144)
dutO1: OXY (Score: 186.7; E-value: 1.8e-57)
dutO2: OXY (Score: 411.4; E-value: 1.3e-125)
dutO4: OXY (Score: 473.7; E-value: 1.6e-144)