BGC0001432 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001432 (Stigmatella erecta)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	42,417	myxochromide D	NRP:Cyclic depsipeptide + Polyketide:Iterative type I	20%

BGC0001432 - Region 1 - NRPS,T1PKS

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Location: 1 - 42,417 nt. (total: 42,417 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 42,418
Label		BGC0001432

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001432.1	1.17	NRP, Polyketide	myxochromide D	Stigmatella erecta
BGC0001023.1	1.05	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001418.1	0.86	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0001833.1	0.86	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001419.1	0.86	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0001128.1	0.83	NRP	luminmide	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.1	0.83	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001844.1	0.82	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002071.1	0.81	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0001417.1	0.80	NRP, Polyketide	myxochromide D	Cystobacterineae bacterium

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001023.1	0.81	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001432.1	0.81	NRP, Polyketide	myxochromide D	Stigmatella erecta
BGC0002071.1	0.71	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0000389.1	0.70	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0001418.1	0.68	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0000056.1	0.67	Polyketide	esperamicin	Actinomadura verrucosospora
BGC0001419.1	0.67	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0001833.1	0.66	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001417.1	0.66	NRP, Polyketide	myxochromide D	Cystobacterineae bacterium
BGC0002075.1	0.65	NRP, Alkaloid	Pyreudione A, Pyreudione B, Pyreudione C, Pyreudione D, Pyreudione E	Pseudomonas fluorescens

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 42417:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (NMe-thr - D-ala) + (leu - X - X - gln)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 15316 - 42417:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NMe-thr - D-ala) + (leu - X - X - gln)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

APZ78852.1

PKS_AT (1064..1355): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 59.1
inactive: 34.1
Methoxymalonyl-CoA: 32.1
Methylmalonyl-CoA: 26.9
Isobutyryl-CoA: 14.6
Propionyl-CoA: 11.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

APZ78853.1

PKS_AT (0..273): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 99.1
inactive: 44.6
Methylmalonyl-CoA: 33.5
Methoxymalonyl-CoA: 33.5
Isobutyryl-CoA: 23.7
fatty_acid: 23.5
Benzoyl-CoA: 9.5
Propionyl-CoA: 9.4
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

mchA

PKS_AT (558..854): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; CE-Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 113.7
Malonyl-CoA: 103.8
Isobutyryl-CoA: 101.0
Methoxymalonyl-CoA: 92.4
Ethylmalonyl-CoA: 82.7
2-Methylbutyryl-CoA: 65.7
Propionyl-CoA: 59.3
trans-1,2-CPDA: 51.4
Benzoyl-CoA: 46.8
CHC-CoA: 41.5
Acetyl-CoA: 38.7
3-Methylbutyryl-CoA: 29.8
fatty_acid: 28.0
inactive: 24.0

mchB

Search NORINE for peptide: strict or relaxed

AMP-binding (510..914): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (2017..2407): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DVWCtAAIiK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 80% (moderate)

mchC

Search NORINE for peptide: strict or relaxed

AMP-binding (468..859): leu

NRPSPredictor2: leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: leu

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 100% (strong)

AMP-binding (1508..1903): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: gaQFIAqVaK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 60% (weak)

AMP-binding (2556..2946): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVWvlAAIiK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 70% (weak)

AMP-binding (3599..3990): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQcGLIDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 90% (moderate)

APZ78858.1

PKS_AT (624..920): pk

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 91.7%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 66.7%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 199.8
Benzoyl-CoA: 126.9
Methylmalonyl-CoA: 113.8
Methoxymalonyl-CoA: 96.8
Ethylmalonyl-CoA: 81.2
Malonyl-CoA: 80.7
CHC-CoA: 50.1
Acetyl-CoA: 48.5
trans-1,2-CPDA: 47.3
3-Methylbutyryl-CoA: 46.4
2-Methylbutyryl-CoA: 38.4
Propionyl-CoA: 35.2
inactive: 32.9
fatty_acid: 17.5

PKS_AT (1058..1354): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 79.2%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 189.7
Ethylmalonyl-CoA: 174.0
Methoxymalonyl-CoA: 144.6
Isobutyryl-CoA: 121.8
Malonyl-CoA: 112.3
Benzoyl-CoA: 109.1
2-Methylbutyryl-CoA: 95.0
trans-1,2-CPDA: 81.0
Propionyl-CoA: 79.5
Acetyl-CoA: 66.9
CHC-CoA: 55.8
3-Methylbutyryl-CoA: 45.6
inactive: 31.9
fatty_acid: 30.8