BGC0001951 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001951 (Cylindrospermum alatosporum CCALA 994)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	58,884	puwainaphycin F / minutissamide A	NRP	100%

BGC0001951 - Region 1 - NRPS,T1PKS

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Location: 1 - 58,884 nt. (total: 58,884 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 58,885
Label		BGC0001951

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001951.1	1.55	NRP	puwainaphycin F, minutissamide A	Cylindrospermum alatosporum CCALA 994
BGC0001950.1	1.26	NRP	puwainaphycin F, minutissamide A	Cylindrospermum moravicum CCALA 993
BGC0001125.1	0.88	NRP, Polyketide	puwainaphycin A, puwainaphycin B, puwainaphycin C, puwainaphycin D	Cylindrospermum alatosporum CCALA 988
BGC0000389.1	0.80	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0002071.1	0.77	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0001844.1	0.72	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001615.1	0.71	NRP	hexose-palythine-serine, hexose-shinorine	Heteroscytonema crispum UCFS10
BGC0001131.1	0.70	NRP	ambactin	Xenorhabdus miraniensis
BGC0001758.1	0.70	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.1	0.68	NRP	icosalide A, icosalide B	Burkholderia gladioli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001951.1	0.94	NRP	puwainaphycin F, minutissamide A	Cylindrospermum alatosporum CCALA 994
BGC0001950.1	0.88	NRP	puwainaphycin F, minutissamide A	Cylindrospermum moravicum CCALA 993
BGC0001125.1	0.76	NRP, Polyketide	puwainaphycin A, puwainaphycin B, puwainaphycin C, puwainaphycin D	Cylindrospermum alatosporum CCALA 988
BGC0000389.1	0.69	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0001758.1	0.69	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001844.1	0.69	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001615.1	0.69	NRP	hexose-palythine-serine, hexose-shinorine	Heteroscytonema crispum UCFS10
BGC0001131.1	0.68	NRP	ambactin	Xenorhabdus miraniensis
BGC0002071.1	0.68	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0001833.1	0.67	NRP	icosalide A, icosalide B	Burkholderia gladioli

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 58884:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (thr) + (thr - asn - thr - asn - D-ala) + (mal - val) + (Me-redmal) + (NMe-asn - pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

puwA

Search NORINE for peptide: strict or relaxed

AMP-binding (513..915): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

AMP-binding (1996..2401): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFmAqVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 80% (moderate)

puwB

PKS_AT (596..894): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.0
inactive: 160.9
Methoxymalonyl-CoA: 68.2
Methylmalonyl-CoA: 59.6
2-Methylbutyryl-CoA: 34.6
Propionyl-CoA: 31.7
Benzoyl-CoA: 30.8
Isobutyryl-CoA: 30.6
Ethylmalonyl-CoA: 30.3
Acetyl-CoA: 28.1
CHC-CoA: 14.1
trans-1,2-CPDA: 12.9
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

puwc

CAL_domain (53..497): pk

minowa: NH2

Prediction, score:

NH2: 198.4
AHBA: 10.2
fatty_acid: 9.2
Acetyl-CoA: 2.7
shikimic_acid: 0.0

puwE

Search NORINE for peptide: strict or relaxed

PKS_AT (610..906): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.1
inactive: 173.7
Methylmalonyl-CoA: 79.9
Methoxymalonyl-CoA: 79.0
Propionyl-CoA: 63.0
2-Methylbutyryl-CoA: 56.3
Isobutyryl-CoA: 44.1
Acetyl-CoA: 38.6
Ethylmalonyl-CoA: 37.8
Benzoyl-CoA: 21.9
fatty_acid: 21.6
CHC-CoA: 20.0
trans-1,2-CPDA: 17.0
3-Methylbutyryl-CoA: 14.2

AMP-binding (2523..2921): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAfWLGGTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 90% (moderate)

puwF-G

Search NORINE for peptide: strict or relaxed

AMP-binding (540..948): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNVGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (1610..2011): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

AMP-binding (2668..3076): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNVGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (3739..4140): asn

NRPSPredictor2: asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: asp, asn
Single AA prediction:: asn

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 100% (strong)

AMP-binding (4801..5194): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 100% (strong)

puwH

Search NORINE for peptide: strict or relaxed

AMP-binding (478..886): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)