BGC0001029 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001029 (Nostoc sp. 152 (Nostoc sp. PCC 9237/1))

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS-like,NRPS	1	55,508	puwainaphycin F / minutissamide A	NRP	22%

BGC0001029 - Region 1 - NRPS,NRPS-like,T1PKS

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Location: 1 - 55,508 nt. (total: 55,508 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 55,509
Label		BGC0001029

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001029.1	2.10	NRP, Polyketide	nostophycin	Nostoc sp. 152
BGC0000389.1	0.97	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0002071.1	0.95	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0001833.1	0.90	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.1	0.86	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.1	0.85	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.1	0.85	NRP	luminmide	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001844.1	0.84	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001758.1	0.81	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.1	0.81	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001029.1	0.93	NRP, Polyketide	nostophycin	Nostoc sp. 152
BGC0000389.1	0.71	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0002071.1	0.70	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0001833.1	0.66	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.1	0.64	NRP	luminmide	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001023.1	0.64	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001825.1	0.64	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.1	0.64	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001844.1	0.64	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001758.1	0.62	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 55508:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal - ohmal) + (mal - D-gln - gly) + (pro - phe - D-ile - pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

npnA

CAL_domain (353..644): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 12.6
shikimic_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

PKS_AT (1947..2258): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 100.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 196.3
inactive: 173.3
Methoxymalonyl-CoA: 50.0
Methylmalonyl-CoA: 46.9
Propionyl-CoA: 30.1
Isobutyryl-CoA: 21.8
Benzoyl-CoA: 17.6
2-Methylbutyryl-CoA: 16.4
CHC-CoA: 10.6
Acetyl-CoA: 10.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4137..4425): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 190.3
inactive: 169.6
Methoxymalonyl-CoA: 54.2
Methylmalonyl-CoA: 46.8
Isobutyryl-CoA: 26.7
Propionyl-CoA: 25.3
Benzoyl-CoA: 21.3
2-Methylbutyryl-CoA: 17.2
Acetyl-CoA: 15.1
CHC-CoA: 13.7
Ethylmalonyl-CoA: 11.1
trans-1,2-CPDA: 9.8
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

npnB

Search NORINE for peptide: strict or relaxed

PKS_AT (566..862): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 95.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.3
inactive: 139.5
Methoxymalonyl-CoA: 70.8
Methylmalonyl-CoA: 66.0
Propionyl-CoA: 54.1
2-Methylbutyryl-CoA: 46.0
Isobutyryl-CoA: 31.2
CHC-CoA: 26.6
Ethylmalonyl-CoA: 25.6
Acetyl-CoA: 21.7
trans-1,2-CPDA: 18.2
Benzoyl-CoA: 16.0
3-Methylbutyryl-CoA: 13.4
fatty_acid: 8.7

AMP-binding (2643..3043): gln

NRPSPredictor2: gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: gln

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQFGLIDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 100% (strong)

AMP-binding (4176..4570): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

npnC

Search NORINE for peptide: strict or relaxed

AMP-binding (545..948): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)

AMP-binding (1610..1999): phe

NRPSPredictor2: phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: phe, trp
Single AA prediction:: phe

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTIAaVCK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 90% (moderate)

AMP-binding (2662..3062): ile

NRPSPredictor2: ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: ile

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGVTFK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 100% (strong)

AMP-binding (4195..4601): pro

NRPSPredictor2: pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: pro, pip
Small clusters prediction:: pro
Single AA prediction:: pro

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code:: pro
Stachelhaus code match:: 100% (strong)