BGC0001332 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001332 (Streptomyces sp. MspMP-M5)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	42,086	BE-43547A1 / BE-43547A2 / BE-43547B1 / BE-43547B2 / BE-43547B3 / BE-43547C1 / BE-43547C2	NRP:Cyclic depsipeptide + Polyketide:Modular type I	33%

BGC0001332 - Region 1 - NRPS,T1PKS

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Location: 1 - 42,086 nt. (total: 42,086 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 42,087
Label		BGC0001332

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001332.1	1.55	NRP, Polyketide	microtermolide A	Streptomyces sp. MspMP-M5
BGC0001050.1	0.77	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0000457.1	0.63	NRP	vicibactin	Rhizobium etli CFN 42
BGC0001876.1	0.62	NRP	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0001187.1	0.60	NRP, Polyketide	xenolozoyenone	Glarea lozoyensis
BGC0001833.1	0.58	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0002057.1	0.57	Polyketide	pateamine	Kiritimatiellaeota bacterium
BGC0001132.1	0.57	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000389.1	0.57	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0001825.1	0.56	NRP	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001332.1	0.95	NRP, Polyketide	microtermolide A	Streptomyces sp. MspMP-M5
BGC0001050.1	0.71	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0000457.1	0.65	NRP	vicibactin	Rhizobium etli CFN 42
BGC0001876.1	0.65	NRP	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0001187.1	0.64	NRP, Polyketide	xenolozoyenone	Glarea lozoyensis
BGC0000057.1	0.63	Polyketide	F9775A, F9775B, orsellinic acid	Aspergillus nidulans FGSC A4
BGC0000107.1	0.63	Polyketide	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001833.1	0.63	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001257.1	0.63	Polyketide	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001906.1	0.63	Polyketide	naphthalene	Daldinia eschscholzii IFB-TL01

Similar known gene clusters

Module view

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NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 42086:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (gly - D-val) + (mal) + (ser) + (ccmal) + (Me-mal - ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

B073_RS40860

PKS_AT (559..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 197.9
Ethylmalonyl-CoA: 194.0
Methoxymalonyl-CoA: 165.7
2-Methylbutyryl-CoA: 126.5
trans-1,2-CPDA: 114.9
Propionyl-CoA: 106.8
Malonyl-CoA: 94.3
CHC-CoA: 81.5
Isobutyryl-CoA: 75.1
Benzoyl-CoA: 55.8
Acetyl-CoA: 32.3
3-Methylbutyryl-CoA: 21.7
inactive: 21.4
fatty_acid: 18.8

PKS_AT (1574..1868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.8
inactive: 99.9
Methoxymalonyl-CoA: 76.0
Methylmalonyl-CoA: 74.8
Propionyl-CoA: 39.4
Isobutyryl-CoA: 33.5
Ethylmalonyl-CoA: 33.0
Benzoyl-CoA: 21.1
fatty_acid: 20.9
2-Methylbutyryl-CoA: 19.7
3-Methylbutyryl-CoA: 18.0
Acetyl-CoA: 17.8
CHC-CoA: 14.7
trans-1,2-CPDA: 12.9

B073_RS0123875

PKS_AT (550..844): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 166.7
inactive: 96.1
Methoxymalonyl-CoA: 77.9
Methylmalonyl-CoA: 76.8
Propionyl-CoA: 65.0
Ethylmalonyl-CoA: 46.2
CHC-CoA: 40.4
2-Methylbutyryl-CoA: 38.3
Isobutyryl-CoA: 37.4
Benzoyl-CoA: 35.6
trans-1,2-CPDA: 28.3
Acetyl-CoA: 28.3
fatty_acid: 21.4
3-Methylbutyryl-CoA: 11.3

B073_RS0123880

Search NORINE for peptide: strict or relaxed

AMP-binding (494..890): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

B073_RS0123885

PKS_AT (556..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 161.3
inactive: 109.6
Methoxymalonyl-CoA: 82.4
Methylmalonyl-CoA: 69.8
Propionyl-CoA: 45.3
Ethylmalonyl-CoA: 39.4
CHC-CoA: 38.3
2-Methylbutyryl-CoA: 35.2
Isobutyryl-CoA: 34.2
fatty_acid: 23.4
Benzoyl-CoA: 23.2
trans-1,2-CPDA: 21.3
Acetyl-CoA: 20.8
3-Methylbutyryl-CoA: 4.8

B073_RS0123900

Search NORINE for peptide: strict or relaxed

AMP-binding (501..893): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQi-MIyK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 70% (weak)

AMP-binding (1559..1959): val

NRPSPredictor2: val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: val

Stachelhaus prediction details:

Stachelhaus sequence:: DAYFlGgTFK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 80% (moderate)