BGC0001705 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001705 (Nostoc sp. CENA543)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS,NRPS-like	1	48,240	microcystin	NRP + Polyketide	76%

BGC0001705 - Region 1 - NRPS,NRPS-like,T1PKS

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Location: 1 - 48,240 nt. (total: 48,240 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 48,241
Label		BGC0001705

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001705.1	2.26	NRP, Polyketide	nodularin	Nostoc sp. CENA543
BGC0000396.1	1.34	NRP	nodularin	Nostoc sp. 73.1
BGC0001016.1	1.32	NRP, Polyketide	microcystin	Anabaena sp. 90
BGC0000426.1	1.09	NRP	sevadicin	Paenibacillus larvae
BGC0001132.1	1.08	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.1	1.08	NRP	luminmide	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.1	1.07	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001844.1	1.05	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001825.1	1.05	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.1	1.04	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001705.1	0.94	NRP, Polyketide	nodularin	Nostoc sp. CENA543
BGC0001016.1	0.76	NRP, Polyketide	microcystin	Anabaena sp. 90
BGC0000396.1	0.68	NRP	nodularin	Nostoc sp. 73.1
BGC0000465.1	0.67	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000041.1	0.64	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001833.1	0.64	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.1	0.64	NRP	luminmide	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001844.1	0.62	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001135.1	0.62	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001434.1	0.62	NRP	nematophin	Xenorhabdus budapestensis

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 48240:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (NMe-thr - X) + (Me-ohmal) + (Me-ccmal - ccmal) + (mal - glu)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 16498 - 48240:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal - ccmal) + (Me-ohmal) + (NMe-thr - X) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ndaF

Search NORINE for peptide: strict or relaxed

PKS_AT (561..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 170.3
inactive: 121.5
Methoxymalonyl-CoA: 54.3
Methylmalonyl-CoA: 47.6
Propionyl-CoA: 44.8
Isobutyryl-CoA: 38.8
3-Methylbutyryl-CoA: 21.1
Benzoyl-CoA: 17.7
Acetyl-CoA: 13.0
CHC-CoA: 11.7
fatty_acid: 10.6
2-Methylbutyryl-CoA: 9.7
Ethylmalonyl-CoA: 8.7
trans-1,2-CPDA: 0.0

AMP-binding (2444..2839): glu

NRPSPredictor2: glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: asp, asn, glu, gln, aad
Small clusters prediction:: glu, gln
Single AA prediction:: glu

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHSGVVGK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 100% (strong)

ndaD

PKS_AT (574..761): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 42.9
inactive: 34.8
Propionyl-CoA: 16.0
CHC-CoA: 12.3
2-Methylbutyryl-CoA: 11.6
Methylmalonyl-CoA: 10.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2632..2916): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.5
inactive: 140.3
Methylmalonyl-CoA: 68.9
Methoxymalonyl-CoA: 67.9
Propionyl-CoA: 40.5
Isobutyryl-CoA: 34.8
Ethylmalonyl-CoA: 27.8
Benzoyl-CoA: 25.8
2-Methylbutyryl-CoA: 24.0
Acetyl-CoA: 21.3
trans-1,2-CPDA: 19.9
CHC-CoA: 17.8
fatty_acid: 13.6
3-Methylbutyryl-CoA: 8.3

ndaC

CAL_domain (90..495): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 20.1
shikimic_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

PKS_AT (1246..1539): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.7
inactive: 131.2
Methylmalonyl-CoA: 77.1
Methoxymalonyl-CoA: 76.5
Propionyl-CoA: 45.0
Isobutyryl-CoA: 39.2
Ethylmalonyl-CoA: 34.7
Acetyl-CoA: 34.5
2-Methylbutyryl-CoA: 31.6
Benzoyl-CoA: 30.9
CHC-CoA: 26.1
trans-1,2-CPDA: 23.0
fatty_acid: 18.9
3-Methylbutyryl-CoA: 14.7

ndaA

Search NORINE for peptide: strict or relaxed

AMP-binding (260..668): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

AMP-binding (2024..2450): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DArHvGifvK
Nearest Stachelhaus code:: glu
Stachelhaus code match:: 50% (weak)

ndaB

Search NORINE for peptide: strict or relaxed

AMP-binding (477..874): X

NRPSPredictor2: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWsFGLvDK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 80% (moderate)