BGC0000365 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000365 (Mycolicibacterium smegmatis MC2 155)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	67,229	glycopeptidolipid	NRP	94%

BGC0000365 - Region 1 - NRPS,T1PKS

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Location: 1 - 67,229 nt. (total: 67,229 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 67,230
Label		BGC0000365

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000365.1	1.06	NRP	glycopeptidolipid	Mycolicibacterium smegmatis MC2 155
BGC0001627.1	0.64	NRP	isonitrile lipopeptides	Mycobacterium tuberculosis H37Rv
BGC0001637.1	0.59	NRP	kleboxymycin	Klebsiella oxytoca
BGC0001724.1	0.56	NRP, Polyketide	oxaleimide C	Penicillium oxalicum 114-2
BGC0000426.1	0.54	NRP	sevadicin	Paenibacillus larvae
BGC0000389.1	0.52	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0002071.1	0.50	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0001748.1	0.50	NRP, Polyketide	pseudospumigin A, pseudospumigin B, pseudospumigin C, pseudospumigin D, pseudospumigin E, pseudospumigin F	Nostoc sp. CENA543
BGC0000430.1	0.50	NRP, Polyketide	spumigin E	Nodularia spumigena CCY9414
BGC0001574.1	0.50	NRP	diisonitrile antibiotic SF2768	Streptomyces thioluteus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000365.1	0.88	NRP	glycopeptidolipid	Mycolicibacterium smegmatis MC2 155
BGC0000364.1	0.70	NRP	glycopeptidolipid	Mycobacteroides abscessus
BGC0000363.1	0.70	NRP	glycopeptidolipid	Mycobacteroides chelonae
BGC0000426.1	0.66	NRP	sevadicin	Paenibacillus larvae
BGC0001627.1	0.65	NRP	isonitrile lipopeptides	Mycobacterium tuberculosis H37Rv
BGC0000389.1	0.65	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0001345.1	0.65	NRP	myxochelin A, myxochelin B	Stigmatella aurantiaca Sg a15
BGC0001637.1	0.64	NRP	kleboxymycin	Klebsiella oxytoca
BGC0002071.1	0.64	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0001724.1	0.63	NRP, Polyketide	oxaleimide C	Penicillium oxalicum 114-2

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 2553 - 67229:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-phe - D-thr) + (D-ala - alaninol) + (ohmal - redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 2553 - 60525:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-phe - D-thr) + (D-ala - alaninol) + (ohmal - redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 26270 - 67229:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-phe - D-thr) + (D-ala - alaninol) + (ohmal - redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

mps1

Search NORINE for peptide: strict or relaxed

AMP-binding (464..850): phe

NRPSPredictor2: phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic
Large clusters prediction:: phe, trp, phg, tyr, bht
Small clusters prediction:: phe, trp
Single AA prediction:: phe

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTtAAICK
Nearest Stachelhaus code:: phe
Stachelhaus code match:: 90% (moderate)

AMP-binding (1961..2362): thr

NRPSPredictor2: thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: thr
Single AA prediction:: thr

Stachelhaus prediction details:

Stachelhaus sequence:: DYWSVGMVHK
Nearest Stachelhaus code:: thr
Stachelhaus code match:: 100% (strong)

mps2

Search NORINE for peptide: strict or relaxed

AMP-binding (46..434): ala

NRPSPredictor2: ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DVWCIAAIYK
Nearest Stachelhaus code:: ala
Stachelhaus code match:: 100% (strong)

AMP-binding (1540..1928): alaninol

NRPSPredictor2: alaninol

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: ala

Stachelhaus prediction details:

Stachelhaus sequence:: DVWYVAAIYK
Nearest Stachelhaus code:: alaninol
Stachelhaus code match:: 100% (strong)

pks

PKS_AT (545..844): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 181.6
inactive: 116.8
Methylmalonyl-CoA: 79.6
Methoxymalonyl-CoA: 69.1
Propionyl-CoA: 53.0
Isobutyryl-CoA: 43.5
fatty_acid: 38.6
2-Methylbutyryl-CoA: 34.9
Ethylmalonyl-CoA: 28.6
Acetyl-CoA: 27.7
Benzoyl-CoA: 27.6
CHC-CoA: 23.5
trans-1,2-CPDA: 21.5
3-Methylbutyryl-CoA: 9.6

PKS_AT (2100..2397): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 185.4
inactive: 114.2
Methylmalonyl-CoA: 85.1
Methoxymalonyl-CoA: 69.0
Propionyl-CoA: 64.3
2-Methylbutyryl-CoA: 42.5
fatty_acid: 34.8
Isobutyryl-CoA: 32.0
trans-1,2-CPDA: 28.1
CHC-CoA: 28.1
Acetyl-CoA: 28.0
Ethylmalonyl-CoA: 27.7
Benzoyl-CoA: 25.9
3-Methylbutyryl-CoA: 9.6

fadD28

CAL_domain (36..476): pk

minowa: NH2

Prediction, score:

NH2: 98.9
Acetyl-CoA: 47.0
fatty_acid: 10.5
AHBA: 3.1
shikimic_acid: 0.0