BGC0001327 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001327 (Micromonospora purpureochromogenes)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS-like	1	38,418	rakicidin A / rakicidin B	NRP:Cyclic depsipeptide + Polyketide:Modular type I	73%

BGC0001327 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 38,418 nt. (total: 38,418 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 38,419
Label		BGC0001327

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001327.1	1.37	NRP, Polyketide	rakicidin A, rakicidin B	Micromonospora purpureochromogenes
BGC0001328.1	0.78	NRP, Polyketide	rakicidin A, rakicidin B	Micromonospora sp. M42
BGC0001023.1	0.63	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0000041.1	0.61	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001331.1	0.59	NRP, Polyketide	BE-43547A1, BE-43547A2, BE-43547B1, BE-43547B2, BE-43547B3, BE-43547C1, BE-43547C2	Salinispora arenicola CNR107
BGC0001330.1	0.58	NRP, Polyketide	BE-43547A1, BE-43547A2, BE-43547B1, BE-43547B2, BE-43547B3, BE-43547C1, BE-43547C2	Micromonospora sp. RV43
BGC0000389.1	0.58	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0002071.1	0.56	NRP	Virginiafactin	Pseudomonas sp. QS1027
BGC0001876.1	0.55	NRP	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0000342.1	0.55	NRP	enniatin	Fusarium equiseti

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001327.1	0.95	NRP, Polyketide	rakicidin A, rakicidin B	Micromonospora purpureochromogenes
BGC0001328.1	0.75	NRP, Polyketide	rakicidin A, rakicidin B	Micromonospora sp. M42
BGC0001023.1	0.66	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0000041.1	0.64	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000389.1	0.63	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0001876.1	0.63	NRP	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0001050.1	0.63	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0001132.1	0.62	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.1	0.62	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0002071.1	0.62	NRP	Virginiafactin	Pseudomonas sp. QS1027

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 38418:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal) + (ohmal) + (ser) + (ccmal) + (NMe-gly) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

IH31_RS0100575

CAL_domain (18..466): pk

minowa: NH2

Prediction, score:

NH2: 41.1
fatty_acid: 25.8
AHBA: 11.2
Acetyl-CoA: 4.3
shikimic_acid: 0.0

PKS_AT (1248..1546): mmal

ATSignature: Malonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 79.2%; Propionyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 152.9
Methoxymalonyl-CoA: 131.8
Malonyl-CoA: 116.7
Ethylmalonyl-CoA: 104.3
Isobutyryl-CoA: 92.7
Benzoyl-CoA: 80.5
2-Methylbutyryl-CoA: 77.0
Propionyl-CoA: 71.9
trans-1,2-CPDA: 63.4
3-Methylbutyryl-CoA: 61.2
inactive: 41.8
fatty_acid: 40.9
Acetyl-CoA: 39.0
CHC-CoA: 38.2

IH31_RS34685

PKS_AT (566..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 173.5
inactive: 124.4
Methoxymalonyl-CoA: 88.2
Methylmalonyl-CoA: 81.3
Propionyl-CoA: 65.0
Isobutyryl-CoA: 57.1
Ethylmalonyl-CoA: 57.0
2-Methylbutyryl-CoA: 56.1
Acetyl-CoA: 46.6
Benzoyl-CoA: 35.5
CHC-CoA: 33.3
trans-1,2-CPDA: 25.5
fatty_acid: 20.5
3-Methylbutyryl-CoA: 10.3

IH31_RS0100585

Search NORINE for peptide: strict or relaxed

AMP-binding (500..914): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLIDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 100% (strong)

IH31_RS0100590

PKS_AT (537..833): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 164.8
inactive: 100.2
Methoxymalonyl-CoA: 79.0
Methylmalonyl-CoA: 67.4
Propionyl-CoA: 45.2
Ethylmalonyl-CoA: 34.7
CHC-CoA: 27.9
Benzoyl-CoA: 25.7
2-Methylbutyryl-CoA: 24.1
Isobutyryl-CoA: 23.1
trans-1,2-CPDA: 20.1
Acetyl-CoA: 19.1
fatty_acid: 18.6
3-Methylbutyryl-CoA: 5.9

IH31_RS0100595

Search NORINE for peptide: strict or relaxed

AMP-binding (494..887): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

IH31_RS0100600

Search NORINE for peptide: strict or relaxed

AMP-binding (505..938): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: pAWcFGvapK
Nearest Stachelhaus code:: gln
Stachelhaus code match:: 50% (weak)

IH31_RS0100615

Search NORINE for peptide: strict or relaxed

AMP-binding (461..863): X

NRPSPredictor2: asp, asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: asp, asn
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLpKIaEiGK
Nearest Stachelhaus code:: asn
Stachelhaus code match:: 70% (weak)